|
 Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc: 
|
|
Glad it worked man!
Quote:
Taking transfers was a bit awkward
Absolutely, there's no way around that. 
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
 Re: Josex' Poke: No Mercy for Bacteria.  [Re: Tankie_J]
#27166642 - 01/24/21 05:26 AM (3 years, 2 months ago) |
|
|
Quote:
Tankie_J said:
APEU buried swab strand taking off nicely. Swab was dipped in sterile water before dissecting. No growth in the pan cyan pucks yet. I did get a plate to germ and may try the poke and shoot it into a puck cup to get invitro fruits for clones.
Thanks for sharing 
|
savan73
Stranger
Registered: 05/05/20
Posts: 252
|
Re: Josex' Poke: No Mercy for Bacteria. *DELETED* [Re: Josex]
#27178164 - 01/30/21 03:44 PM (3 years, 2 months ago) |
|
|
Post deleted by savan73
Reason for deletion: .
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: savan73]
#27178191 - 01/30/21 04:14 PM (3 years, 2 months ago) |
|
|
Among other things, this tek is intented for severe or persistent bacterial contamination that is difficult or impossible to get away from by transferring alone.
In most cases you can clean almost anything by taking timely and precise transfers. We'd need a better pic to know for sure but from here that doesn't look like it's contaminated and if it is I don't think it's bad.
If you want to transfer to pucks you can do it but likely you can clean it by transferring normally to new agar plates. Also, you need to let that plate grow out a bit more.
|
Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ
Registered: 06/13/09
Posts: 4,900
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Josex]
#27196109 - 02/09/21 06:38 PM (3 years, 2 months ago) |
|
|
Rip... tried this tek with a ugly plate and it just turned into trich... zzzz.
Poked to the nearest edge where it was mostly white as well 
--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |
  
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Blazer420]
#27196130 - 02/09/21 06:49 PM (3 years, 2 months ago) |
|
|
Shit happens, it's not tek related 
If you poke trich you'll get trich and if you fumbled your technique you will get green too.
|
Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ
Registered: 06/13/09
Posts: 4,900
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Josex]
#27196169 - 02/09/21 07:11 PM (3 years, 2 months ago) |
|
|
Yeah true. Having trouble with a dirty spore print.. Was hoping this would fix it up. Guess its back to the drawing board 
--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Blazer420]
#27196222 - 02/09/21 07:38 PM (3 years, 2 months ago) |
|
|
Mold issues from that print or bacteria?
|
Blazer420
ŦøжїϿ ÐȐȜȧƜƐȓ
Registered: 06/13/09
Posts: 4,900
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Josex]
#27196251 - 02/09/21 07:54 PM (3 years, 2 months ago) |
|
|
Well the print wasn't packaged very well.. Literally only half the edge of the foil was printed, and I have placed it straight onto agar with barely any growth going on. (Albino species btw)
The growth I have seen has been really small, one plate just turned into a small black blob, another plate seemed to be mycelium growth, but a few days down the track it started to turn blue/black near the middle. So I thought maybe I could just give it the ole poke closest to the edge where the majority of it was white, I would be getting somehwere... But like you said it must of just been straight trich..
I ended up making the print into a MSS and also put a few drops on some plates to see if getting the spores a little moist would help it produce some proper mycelium growth. Just a waiting game at the moment with the MSS > agar.
--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |
Edited by Blazer420 (02/09/21 07:55 PM)
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: Josex' Poke: No Mercy for Bacteria. [Re: Blazer420]
#27196263 - 02/09/21 08:01 PM (3 years, 2 months ago) |
|
|
I see. What I'd do in your case is inoculate a big number of agar plates to raise the odds of getting actual cube myc. Use an inoculation loop to take the spores from the print and then draw a zigzag pattern on the agar.
It does sound like you poked mold indeed. 
|
 infestedpasta
Stranger
Registered: 09/20/07
Posts: 128
Last seen: 20 hours, 59 minutes
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
#27210340 - 02/17/21 01:15 AM (3 years, 1 month ago) |
|
|
Got some weird slime that wont go away.. going to try this tomorrow.
|
bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 3,733
Loc: Gamehenge
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: infestedpasta]
#27233765 - 03/02/21 02:11 PM (3 years, 1 month ago) |
|
|
I just found a pretty large in-vitro GT in one of my puck jars I'll snap a pic later when I clone that MF.
These pucks are awesome. Thanks again for sharing, Josex!
--------------------
 I  5318008 NOT a virgin!
|
bongoman
Stranger
Registered: 09/19/18
Posts: 558
Last seen: 18 days, 6 hours
|
|
I’m using this method to clean up some dirty syringes. Thoughts on how many days it might take for spores to germinate on this substrate?
|
bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 3,733
Loc: Gamehenge
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: bongoman]
#27234117 - 03/02/21 06:04 PM (3 years, 1 month ago) |
|
|
It's about the same as agar. a few days to a couple weeks IME. My syringes were super fresh so I think they all kicked off in 4 or 5 days
--------------------
I 5318008 NOT a virgin!
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
|
PE blobbing the fck up on a puck.
|
bigfootscreepyuncl
Stranger
Registered: 11/15/20
Posts: 3,733
Loc: Gamehenge
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex] 1
#27272987 - 03/28/21 11:13 AM (3 years, 19 days ago) |
|
|
Don't feel bad, Josex..I've got PE blobbing the fuck up on 7 shoeboxes
--------------------
I 5318008 NOT a virgin!
|
StonedCatRescue
Person
Registered: 12/01/20
Posts: 67
Last seen: 1 year, 7 months
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: PNat]
#27329313 - 05/30/21 02:40 PM (2 years, 10 months ago) |
|
|
Quote:
PNat said:
Thanks for this, I started agar for about 2 weeks now while waiting on my BRF cakes. I been doing transfer after transfer, away from bacteria...now to the point where I don't even know why I am doing agar for. Wasting money on petri dishes and time making agar.
This is what I'm thinking...
If, as Jo says, this method usually results in clean myc after the first extraction from the Verm mix, wouldn't this be a viable alternative to doing agar work?
|
Ajeje Brazo
Stranger
Registered: 05/24/21
Posts: 18
Loc: italy 
Last seen: 1 year, 10 months
|
|
Thank you Josex for sharing your experience! 
I am new to the forum and still in the “study” phase, my main concern is about contamination, since I live in a moldy house.
So I am trying to fit my strategy to minimize this risk.
I think I am gonna buy a spore syringe when ready, and I am thinking about using your Biopsy Tek to “filter” contaminants, basically avoiding the “spore syringe to agar” step, and hopefully also the following agar “purification” steps, before inoculating agar-to-grain.
(I will try the full PF Tek too, anyway)
My hope is to get at least one flush, being able to close the circle with mycelium and spore storage.
I read everybody’s posts carefully, got a lot of info and now I am trying to put together in a coherent plan my scattered ideas about how to fit this tek in..
I would like to share my work hypothesis, to get any suggestions, or to correct me if I got something wrong..
1 - squirt spore syringe to brf puck “A”.
2 - when brf puck “A” is more than half colonized (to allow possible hidden contams to show) poke its healthy leading edge and transfer to brf puck “B”
3 - when brf puck “B” pins, take one pin and transfer on agar.
(Steps 1 to 3 I’d do in series, maybe 10 pucks..)
4 - agar should be clean at this point.. (if necessary transfer and purify.. this I hope to avoid or minimize with this Tek)
5 - expand on agar only if necessary (depending on how many clean petris/pasties I’ve got)
6 - put big pieces of agar wedges on grain masters, to have a head start on any possible remaining molds/bacteria.
I hope to have in this way no step where molds and bacteria are favoured to propagate.. I don’t know..
I’m counting on the fast colonizing speed of pins, but I’m not sure especially of step 3: do you think it’s better to put on agar a poke of brf puck “B” the way it was done for “A”.. instead of using one of its pins?
If I didn’t fuck up somewhere, the pins should be rather clean, since grown in vitro..
In case you agree with step 3, I was wondering if the adding of casing verm can be avoided without hindering pinning too much, in this particular case, since the risk of contamination increases, having to reopen the jar.
thank you for any suggestion!! 
--------------------
no real goals.. just intense playing sometimes
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: Ajeje Brazo]
#27329394 - 05/30/21 04:00 PM (2 years, 10 months ago) |
|
|
Your plan sounds good. Pucks are very good for germinating spore solution.
You can even do LC by poking puck B without issues.
No need to let them pin either. You can transfer directly to agar before it pins and then keep the puck around until it fruits so you can take an invitro pin from it.
Never add a verm casing if you don't want to compromise sterility, ain't needed either.
These days I don't take invitro pins. If I want to clone something I do it from fruits from a tub so I can see clearly what I'm cloning and can also make sure I can replicate those same conditions when I grow out the clone in subsequent grows.
|
Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
|
Re: The Biopsy Method: No Mercy for Bacteria. [Re: StonedCatRescue]
#27329400 - 05/30/21 04:07 PM (2 years, 10 months ago) |
|
|
Quote:
StonedCatRescue said:
Quote:
PNat said:
Thanks for this, I started agar for about 2 weeks now while waiting on my BRF cakes. I been doing transfer after transfer, away from bacteria...now to the point where I don't even know why I am doing agar for. Wasting money on petri dishes and time making agar.
This is what I'm thinking...
If, as Jo says, this method usually results in clean myc after the first extraction from the Verm mix, wouldn't this be a viable alternative to doing agar work?
Yes it is viable, it's all I do lol
I'd prefer agar myself because it's much easier to make, less messy.. but I do pucks because they grow much more slowly than agar and that reduces my workload tremendously. Keep one puck per culture at a time, noc one day via biopsy, forget about it for 3-4 weeks until they grow out enough that they need a tranfer.
My Agar game gets pretty crazy everytime, would rather do this.
|
|
Threaded
Previous
Index
Next
Maeng Da Thai Kratom Leaf Powder 
Red Vein Kratom 
Bulk Substrate 
APE Liquid Culture For Sale 
Buy Kratom Extract 
Certified Organic All-In-One Grow Bags 
Liquid Cultures 
Bulk Cannabis Seeds
Edit 
Reply 
Dvoid
1 2 3 all )
MisterKite
upupup
Search59