pacmanbreed said:

I have a pinning coir puck innoc 21 days ago thats fast.
Is there a possibility bacteria can be enmeshed within the pin body?
Im plannin to clone this guy.


1 out of 10 did pin, some stalled, some are fully colonized but with something piggy backing bothering the mycleium enough. Man my samples are bacterial AF.

---

Try pf-tek sub next time, I think I told you coir is not nearly as effective to deal with the bitch? can't remember if it was you. And yea clone that pin.

Jhova, glad to hear that man. Now to an awesome grain prep and lotta shrooms.

pablokabute said:

can this work on grain pucks?



brf is a pita to get where I'm based...

---

Quote:

As I was reading it though, it seemed as if the method was meant to take microscopic pieces of tissue from an agar plate that though has contams, still has mycelium growing, and to then take that and inject it onto a brf puck. Was I understanding it correctly, or can I take the contaminated syringe and use on the brf to help save the 5 cc's of spores I have left?

---

Quote:

I snagged a few pieces of the colonized grain and stuck them on some agar so if the syringe needs to be tossed, all is not lost.

---

That's actually a very good idea.

Josex said:

I happened to read the thread you made where you were explaining the problems you were having and saw that you wiped the needle after flaming, never do that.
Everything on the needle gets killed when you flame, wiping after flaming is a good way to make the needle unsterile again.

That could explain why you're having issues with bacteria, although it could also be that the syringe is dirty, but you'll never know for sure till you quit doing that bad practice.

Quote:

As I was reading it though, it seemed as if the method was meant to take microscopic pieces of tissue from an agar plate that though has contams, still has mycelium growing, and to then take that and inject it onto a brf puck. Was I understanding it correctly, or can I take the contaminated syringe and use on the brf to help save the 5 cc's of spores I have left?

---

Yes you understood correctly, but since you didn't get any growth on the plates you can also use the syringe on some pucks. Spore solution germinates easy on pf-tek substrate without the mess you can get on agar. Just flame the needle, let it cool for a few seconds and squirt a couple drops in each puck, you don't need to flame between inoculations, you could do 2 or 3 pucks before flaming again. When you get growth drop by with any questions you might have on what to do next.

Quote:

I snagged a few pieces of the colonized grain and stuck them on some agar so if the syringe needs to be tossed, all is not lost.

---

That's actually a very good idea.

---

more4u2c said:

Here is one that when I was grabbing wedges a drop of soapy bleach water from the top of the SAB dripped into the damn plate so I closed it up and put it to the side to toss and noticed that it turned the rizo part blue but the fluffy parts not hurt so I'm assuming the fluffy stuff is my contam I'm fighting

---

Hard to tell from that pic but I think I see like a strange ring of growth on the edges of the culture. It could be meshed-in mold.
Looks something like this?

In the pic myc stopped growing and meshed-in mold got ahead and started colonizing the edge of the plate, eating the food the color in the process.

Does it usually show up when the plate is more than 50% colonized?
Have you noticed any weird shapes of non-colonized agar, like perfect little triangles?
Like this:

Mateah said:

Check out this weird growth on this Mazatapec culture

---