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Invisibleperuvian spark
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Registered: 02/03/03
Posts: 673
Really quick cloning question
    #2397617 - 03/03/04 10:26 AM (13 years, 3 months ago)

Quick question--
Can you put a cap in the fridge overnight if you can't take a tissue sample until the next day? I just picked a mushroom that I'd like to clone, but I can't get the PDA petri dishes until tomorrow afternoon. So I put the cap in a ziploc and ive got it in the fridge, should it be OK? ( I looked through the FAQ's and I can't find anything on storing tissue ) Thanks all.  :mushroom2:


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Offlineatomic1
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Registered: 09/18/03
Posts: 1,105
Loc: Appalachia
Last seen: 3 months, 2 days
Re: Really quick cloning question [Re: peruvian spark]
    #2397894 - 03/03/04 12:22 PM (13 years, 3 months ago)

If its just overnight i'm sure it'll be ok. Don't worry about it


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OfflineAmazonMatt
Mushie muncher

Registered: 07/27/03
Posts: 58
Last seen: 5 years, 3 months
Re: Really quick cloning question [Re: peruvian spark]
    #2399633 - 03/03/04 07:08 PM (13 years, 3 months ago)

Do to my work hours for 4 days out of the week I have no time to do anything. Well I had a Amazon casing mature on me at the beginning of my four days and I wanted to clone one of the bigguns. So I pulled it up put it in some Gladware, shoved it in the fridge and 4 days later I pulled it out and cloned it. Worked like a charm!


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InvisibleCoolBlue
n00b
Registered: 09/16/03
Posts: 619
Re: Really quick cloning question [Re: peruvian spark]
    #2400079 - 03/03/04 09:18 PM (13 years, 3 months ago)

yeah as long as the cap hasnt started rotting then you should be fine to start a clone :laugh:


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,000
Loc: USA Mountain Northwest
Last seen: 2 months, 23 days
Trusted Cultivator
Re: Really quick cloning question [Re: peruvian spark]
    #2400107 - 03/03/04 09:24 PM (13 years, 3 months ago)

You'd probably have better luck getting your tissue sample from the stipe than the cap in this instance. Hold the mushroom upside down and tear the stem in half from the base to the cap. Don't use a knife, just tear it. This will expose some 'virgin' territory in the center of the stem. Take your clone from there.

amazon,
we must have similar shifts. I work four days on, four days off. 12 hour days. The four days on kinda sux. I like getting four days off at a time though.


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