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Invisibleagar
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TURBINADO LC * 1
    #6378527 - 12/17/06 04:24 AM (14 years, 11 months ago)


PC'ed 12 minutes.
Very clear & just slightly gold tinted.
(no sediment)
Will inoculate after it's cooled.
And, see how it works.


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Offlineetam
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Re: TURBINADO LC [Re: agar]
    #6378598 - 12/17/06 05:26 AM (14 years, 11 months ago)

This will be interesting.


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OfflineJonnyDeformed

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Re: TURBINADO LC [Re: etam]
    #6378612 - 12/17/06 05:41 AM (14 years, 11 months ago)

If this works then there would be no reason why my experiment idea won't. I've been thinking about spawning to sugar cane mulch since i have a pile. What do you think?


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dubiousness
Dubious compound

it is dangerous to be right in matters on which the established authorities are wrong.
A penalty for possession of a drug/plant should not be more damaging than the drug/plant itself.


Edited by JonnyDeformed (12/17/06 05:42 AM)


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Invisibleohmatic
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Re: TURBINADO LC [Re: JonnyDeformed]
    #6378628 - 12/17/06 06:14 AM (14 years, 11 months ago)

hey agar, just cause i spot this lc post.

about the longterm storage of liquid myc, iirc you mentioned (once the lc is ready to use)
to use sterile needles to get out as much LIQUID (not myc) as possible.

then squirt in sterile water (and maybe wait some days till the myc digested
any still remaning nutes)
draw a syringe with myc and squirt into lil jar filled with sterile water -> refrig.

it was about that, wasit ?


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

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Invisibleagar
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Re: TURBINADO LC [Re: ohmatic]
    #6378639 - 12/17/06 06:30 AM (14 years, 11 months ago)

For long term storage of mycelium from an LC.

Best bet is to inject into a test tube, w/port or rubber stopper.
Or, a vacutainer.
Then stand the tube upright.
So the mycelium will settle to the bottom.
Then, aspirate out as much liquid as possible.
Then, inject sterile distilled water.

Object is to remove as much nutrient as possible.
Then, refrigerate the tubes.

Lacking air, nutrients & refrigerated.
Mycelium in liquid suspension will store for a considerable time.

Another method RR brought to light.
Is to colonize a pint or quart jar of wbs/grains.
But, have jar only 1/4 to 1/3rd full of spawn.

Then, inject sterile distilled water.
Shake the jar violently.
(to dislodge mycelium from wbs/grains).
Then, aspirate out mycelium into syringes.
For long term refrigerated storage.
That way, there is no nutrients in the syringe.


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InvisibleBlutjager
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Re: TURBINATE LC [Re: agar]
    #6378753 - 12/17/06 09:46 AM (14 years, 11 months ago)

I always see those little packets of "Raw" sugar,I look forward to see how this pans out


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InvisibleBlutjager
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Re: TURBINATE LC [Re: Blutjager]
    #6378795 - 12/17/06 10:33 AM (14 years, 11 months ago)

How many packets did you put into how much water ??


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OfflineTurdFerguson
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Re: TURBINADO LC [Re: agar]
    #6378869 - 12/17/06 11:28 AM (14 years, 11 months ago)

Quote:

agar said:


Lacking air, nutrients & refrigerated.
Mycelium in liquid suspension will store for a considerable time.





What would a considerable amount of time be, relatively? Is there a ballpark window of time you have discovered before they would expire?

Thanks,

DD


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Offlinetotal
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Re: TURBINADO LC [Re: TurdFerguson]
    #6378885 - 12/17/06 11:38 AM (14 years, 11 months ago)

well dickdiver...id say at least a yr...iv heard of longer when stored on slants though...


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OfflineVibrate
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Re: TURBINATE LC [Re: Blutjager]
    #6378930 - 12/17/06 12:03 PM (14 years, 11 months ago)

Convenient premeasured sugar packets. If it works out, I always like convenience. Good luck.


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Invisibleagar
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Re: TURBINATE LC [Re: Vibrate]
    #6380056 - 12/17/06 07:28 PM (14 years, 11 months ago)

I used a 5 gram packet, into about 220 ml of distilled water.
Heated the jar of water in the microwave.
Dumped in the packet content & stirred it.
To dissolve the little crystals.
Then, added a lid & PC'ed.
Cooled overnight, inoculated with about 2 ml spore solution.
No stir bar.
Incubated around 75F a few days.


Small myc cloud is developing.
(hard to photograph)

Object was to just see if it would germinate/colonize.
Will test more & adjust for optimal solution.

$0.69 cent box contains 25 packets @ 5 grams each.


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Offlinetotal
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Re: TURBINATE LC [Re: agar]
    #6380096 - 12/17/06 07:38 PM (14 years, 11 months ago)

good job agar...sugar in the raw...would of never thought of that...reminds me of the time i was a n00b and tried to use table sugar as an additive in my agar!! :rolleyes:


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OfflineHypercube
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Re: TURBINATE LC [Re: total]
    #6380993 - 12/17/06 10:24 PM (14 years, 11 months ago)

Good to see this works!!
I'll try this instead of honey next time, have loads of raw sugar hanging around.


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Offlinekjb1891
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Re: TURBINADO LC [Re: agar]
    #6383926 - 12/18/06 07:13 PM (14 years, 11 months ago)

What about adding something like gelatin in with the sugar for some amino acids?


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Invisibleagar
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Re: TURBINADO LC [Re: kjb1891]
    #6383959 - 12/18/06 07:20 PM (14 years, 11 months ago)

Quote:

kjb1891 said:
What about adding something like gelatin in with the sugar for some amino acids?



Nope, not a good idea.
Think about it.


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Invisibleagar
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Re: TURBINADO LC [Re: kjb1891]
    #6385309 - 12/19/06 05:23 AM (14 years, 11 months ago)

:grin: :thumbup:


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OfflinePrimate
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Re: TURBINADO LC [Re: agar]
    #6385394 - 12/19/06 07:10 AM (14 years, 11 months ago)

Quote:

agar said:
:grin: :thumbup:




VERY NICE!  and bookmarked.

you are truly an innovator Agar :thumbup:

Much love.

-Prim


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"Power to the peaceful"

-Micheal Franti and Spearhead


Edited by Primate (12/19/06 07:12 AM)


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Invisibleagar
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Re: TURBINADO LC [Re: Primate]
    #6385401 - 12/19/06 07:13 AM (14 years, 11 months ago)

LOL, this was the first go.
The one I started with.
5 gram packet to about 220 ml distilled water.


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OfflineVampireSlayer
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Re: TURBINADO LC [Re: agar]
    #6385414 - 12/19/06 07:22 AM (14 years, 11 months ago)

nice! turbinado sugar ftw! now we don't have to mess with sticky syrup anymore!


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Re: TURBINADO LC [Re: VampireSlayer]
    #6387723 - 12/19/06 09:03 PM (14 years, 11 months ago)

awesome work agar, ths will be my new lc additive once this bag of tan malt turns into a rock


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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