Essential Solutions 101 - Advanced Mycology

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Maeng Da Thai Kratom Leaf Powder

Substrate Bags

Buy Kratom Extract

Isolated Cubensis Liquid Culture For Sale

Unfolding Nature: Being in the Implicate Order

All-In-One Bags That Don't Suck

Jump to first unread post

Pages: 1 | 2 | 3 | Next > [ show all ]

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

Joshua
Holoman

Registered: 10/27/98
Posts: 5,398
Loc: The Matrix

Essential Solutions 101

#2299400 - 02/04/04 12:59 AM (20 years, 2 months ago)

I will provide what I think are 3 essential solutions to the cultivation practice.

1) Hydrogen peroxide:

I use a 1.5 % solution of hydrogen peroxide (H2O2) to prevent the germination of contaminant spores. I spray casings after spawning, anytime I open them to inspect, before and after applying a casing layer, and after picking each flush. H202 oxidizes (damages) any organic tissue. Many organisms produce enzymes that help break down H2O2 into water and oxygen, bubbles are an indication of this activity.These enzymes are known as peroxidases. Spores, because they are dormant and not in a metabolic state, do not produce these enzymes. This means that H2O2 will damage their tissue and prevent them from germinating. H202 should be used as a preventative and maintainance solution. Preventative in the respect that it wil prevent germination of spores, maintainance in that regular and proper use will help to maintain a healthy growth environment.

Be careful and understand that some tissue damage will occur with this solution. Do not apply to primordia or developing pins, as a high rate of aborts will likely be noticed.

2) Alcohol:

I use a 35% solution of isopropyl alcohol (rubbing alcohol) as a topical antimicrobial. I regularly clean my fruiting chambers with this solution to kill spores and bacteria. I spray the surface to be cleaned with a liberal spraying of the solution, I then wait at least five minutes and dry with a clean towel.

I also use this solution as a microbial aerosol. I prefer to use this solution over Lysol and other common microbials. Alcohol will not leave a perfume residue on surfaces. Alcohol readily evaporates. Alcohol has far fewer carcinogens present in its makeup. I spray the immediate area I am to work with including the air in order to reduce the ammount of any contaminants present. The droplets of solution will bind many airborn particles and render them (in most cases) sterile.

When I use a foil print or another well sealed print. I will spray the outside of the print surface and let sit for at least 5 minutes for best results. This will disinfect the outside surface of the print and help to insure success with any spore transfers.

Be cautious with this solution. A solvent grade air filter should be worn in the case of repeated exposure. Avoid inhaling the mist or fumes from this solution

3) Bleach

I use a 5% solution of houshold bleach (.3% actual sodium hypochlorite) as a surface disinfectant. I spray the surface, wait 5 minutes and wipe clean. in my fruiting containers I usualy use a bleach solution as a primary disinfectant, wipe clean, and then use alcohol as my secondary disinfectant.

Again, do not inhale any vapors from this spray...it hurts!

__________________________________________________

For the above dilutions.

1) H2O2 Household 3%

Mix 1:1

2) Alcohol Household (Isopropyl) 70%

Mix 1:1

3) Bleach Houshold 6%

Mix 1:20

I wish you the best of luck with the right of knowledge.

Joshua

Extras:

Similar Threads	Poster	Views	Replies	Last post
How do I extract psilocybin with alcohol?	Rudiger420	18,645	16	06/18/03 05:48 PM by Quick Blaze
recipe for peroxide? ( 1 2 all )	sleepingbigguy	3,512	22	04/19/05 09:18 PM by Abermelin
Cheap Anhydrous Alcohol for Extraction ( 1 2 all )	brainwreck	5,438	27	10/20/02 03:08 PM by blahblahblah
Alcohol Extraction Revisited ( 1 2 all )	netrunner	18,429	26	04/05/20 08:49 AM by trustintheblock
dH20 vs. an isotonic solution for long term storage	Aeolus1369	2,250	3	04/08/05 05:49 PM by Suntzu
Liquid Nutrient Solution ( 1 2 all )	YidakiMan	5,141	35	07/18/03 09:37 AM by mycofile
Honey water vs. malt extract solution?	cactus	5,142	17	03/01/05 08:42 PM by kingkc
Extra light malt extract myc solution questions	Pugslee_Atoms420	1,247	2	03/31/03 10:42 PM by Pugslee_Atoms420