Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Scales, pH Test Strips

Jump to first unread post. Pages: 1
OfflineAeolus1369
Dr. Seahorse
Male

Registered: 05/20/02
Posts: 367
Last seen: 7 years, 9 months
dH20 vs. an isotonic solution for long term storage
    #4021427 - 04/06/05 01:48 PM (11 years, 8 months ago)

I've read many posts that recommend keeping mycelial cultures in distilled water for long term storage. But have there been any tests done to see if an isotonic solution (one where the concentration of solutes in the solution is equivalent to that inside the mycelium) might be optimal?

Many bacteria and eukaryotic cells will lyse in dH20 because the water diffuses through the cell membrane and generates too much pressure for the membrane to withstand. I know fungi circumvent this because their thick cell-wall can withstand high internal pressure, but perhaps on longer time scales it becomes an issue?

Admittedly this isn't such a huge concern since the "several years" of viability I've seen claimed with just dH20 is probably long enough for practical purposes. Still though, optimization is a recurring theme here so I was just curious if anyone knew anything about the significance of water activity in the culture solution.

--Aeolus


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 1 month, 2 days
Re: dH20 vs. an isotonic solution for long term storage [Re: Aeolus1369]
    #4025311 - 04/07/05 10:29 AM (11 years, 8 months ago)

I've thought about this a little bit too. The 'experiments' take years to perform, unfortunately.
I think the cell walls should be pretty resistant to osmotic lysing, but I'm not so sure about the wide pH swings that dH2O can go through. Because it's so easy to come by in labs, I've always wanted to try TE buffer; in addition to being somewhat osmotically balanced, it's pH balanced and contains EDTA which curbs rogue enzymatic and bacterial activity. You can use 0.1X TE buffer for many applications as well.
dH2O has worked well for most species I've played with. It seems like the slower growing/finicky species don't survive as well, though. It has never worked for pans, e.g., [IME]


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAeolus1369
Dr. Seahorse
Male

Registered: 05/20/02
Posts: 367
Last seen: 7 years, 9 months
Re: dH20 vs. an isotonic solution for long term storage [Re: Suntzu]
    #4025820 - 04/07/05 01:09 PM (11 years, 8 months ago)

Hmm, interesting. TE uses Tris as a buffer right? I was thinking the other day how a strong buffer like tris might be used in a casing to ensure long term pH balance but I have no idea if it has any negative impact on mycelium. I would imagine EDTA is to be avoided because it would render many of the mycelium's secreted enzymes inactive but I think it would be beneficial in a long term storage solution as you said.

How does dH20 in a sealed container undergo wide pH swings?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 1 month, 2 days
Re: dH20 vs. an isotonic solution for long term storage [Re: Aeolus1369]
    #4031936 - 04/08/05 07:49 PM (11 years, 7 months ago)

dH2O straight from the source doesn't necessarily have a pH of 7. It depends on a lot of factors. If left to equilibrate it usually settles around pH 5.0-6. I'd imagine even a little biological activity could drop it furthur; maybe a buffering agent could keep it constant and closer to optimal.
I can't find the source, but somewhere I heard that ultra-pure water ['ddH2O'] can have an initial pH of something like 3-9; Most likely any water will settle to 5-6 over a short period of time, though.

So I wonder what 'optimal' really would be?


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Scales, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* practical method for long term storage of mycellium in dH2O
( 1 2 all )
Quincunx 4,707 23 02/15/04 03:23 PM
by Anno
* Experience with long-term storage in distilled water?
( 1 2 all )
ragadinks 3,661 26 10/25/03 11:16 PM
by Cow Shit Collector
* Preparing jars for long term storage before use. Baby_Hitler 1,485 9 02/23/04 08:04 PM
by Baby_Hitler
* storage of liquid mycellial cultures gdr 900 2 01/02/03 12:06 PM
by Anno
* Anyone thought of or tried this for storage.. Cubies4Me 675 4 07/27/02 02:08 PM
by SixTango
* Culture storage Cyber 3,309 18 05/22/08 12:38 PM
by 420inmyapt
* Mycelium's very long storage: my dilemma.
( 1 2 all )
FreeSporePrints 3,281 23 12/24/08 10:21 AM
by denger
* Storage Bruiser 766 3 03/27/03 04:24 AM
by Bruiser

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,697 topic views. 0 members, 4 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Kratom Eye
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.004 seconds on 14 queries.