cronicr said:

cubes don't need rattle snake venom, the spores have no clue that they are b positive or cambodian, for cubes it can be as simple as printing one on top of another and cloning the desired fruit or putting two isolates together on a plate.
rr has not been here for a long time either...what you planning here?

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drake89 said:

i've been researching this too.  all of the literature I can find that actually has detailed procedure uses PEG (wayyy cheaper than venom) to make protoplasts.  About the only paper available for free talks about P. florida and C. indica hybridizing which sounds like a pretty big gap.

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Psilocybimater said:

You could try chitinase or buy a rattlesnake<$400 at a reptile show, although depending on species it could have hepatoxic, neourotoxic, cytotoxic, myotoxic venom. There are like 15+ enzymes in rattlesnake venom, it would probably be cheaper to buy the enzymes and experiment with them.

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myceliups said:

PEG is cheap and can be made easily in the lab. Chitinase may be a option as well. As for venom. I have a friend with venomous creatures. I can get free venom from him. A rattlesnake I forget the species and a gaboon viper. So I have my venom soure but what would my next step be. Make my agar dish and put my two fungi samples down and when they get close to reaching then put the venom down. I think that may be the easiest method. Maybe?

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