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First mold on colonizing BRF cakes?
    #22611760 -

I've got five 1/2-pint jars colonizing. Everyone looks happy except this one:



Am I right that it's contaminated?

If so, am I right that I ought to move it from the others?

If so, am I right that I ought to trash it carefully rather than try fruiting somewhere else? (Outside is not an option, as winter is setting in.)

Thanks!

Edited by Groovy Hobbit (12/04/15 08:44 AM)

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22612281 -

looks like bacteria:thumbup: you can try n fruit it but i wouldn't be hopefull


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I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: First mold on colonizing BRF cakes? [Re: cronicr]
    #22614491 -

Is the bacteria the yellowish areas, the slimy looking areas, or both?

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Re: First mold on colonizing BRF cakes? [Re: browny]
    #22614500 -

More or less just everything, gives the myc the melted ice cream look.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: First mold on colonizing BRF cakes? [Re: cronicr]
    #22614521 -

Yeah. I'll give it a shot in an out of the way place. And I won't expect anything.

Thanks for the feedback!

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22614540 -

I wpuld fruit it right out of the jar.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: First mold on colonizing BRF cakes? [Re: cronicr]
    #22614553 -

I'll give that a try.

Is that a better strategy for times like these?

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22614606 -

Would you fruit it straight out of the jar, now?

And without an overnight dunk?

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22614623 -

I would still dunk and yep fruit it now.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: First mold on colonizing BRF cakes? [Re: cronicr]
    #22614779 -

There. It's dunking in the fridge.

I flaked at least some of the bacterial infection(?) off, down to what might be clean, colonized substrate. Fingers crossed.

And thanks, Shhhhh

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Re: First mold on colonizing BRF cakes? [Re: cronicr]
    #22615885 -

What I had looks like the picture of bacterial infection in a BRF cake jar from http://www.shroomery.org/forums/showflat.php/Number/17231150.

Is what I'm looking at in my mycelium bacterial?

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22627703 -

I have ANOTHER jar from the same group that's just showing trouble, perhaps of the same kind. So I'd like to say something about my setup in case it helps diagnose.

I've got a dome over this 10" x 20" garden tray, with airstones in under the hydroton. I've got it on a heating mat. So the temp is 67 - 75F and the humidity is 60% - 70%.


The jars generally look good:


But I think it was 3 days ago that I removed the first infected jar. I birthed and scraped the grey gunk off this cake (the one that started this thread). I moved it downstairs, giving it its own humidity dome. Is the white fuzz  in these marco-photos cobweb mold? Different facets are visible in each enlarged image:



The rest of the mycelium isn't fuzzy:


-----------------
Now here's another wayward cake:


Is it bacteria? Or mold?

Should I remove this jar? And try birthing it somewhere else? Should I spray it down with H2O2?

Any ideas what's going on? This discoloration wasn't visible 2 days ago! What might cause that in a mostly-colonized cake?

Edited by Groovy Hobbit (12/08/15 07:54 AM)

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22628843 -

everything your doing for colonization is wrong. Just let your jars sit on a shelf. They will do fine :thumbup: don't worry about temps or humidity (or anything really) for colonization. That comes later.


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Re: First mold on colonizing BRF cakes? [Re: PsilocyBen17]
    #22632992 -

So is there no need to be concerned with humidity outside the jars during colonization - even jars like mine, with a polyfil hole in the top for gas exchange? and even if the ambient humidity is around 30 - 40%? If these are non-issues, thanks; my concern was misplaced.

As to temp., though, I've read on these forums that "80-83F is optimal growth incubation temperature" (e.g., here, attributed to RogerRabbit). In winter, without a heating mat, my colonizing temps go down to the 60s, perhaps on cold nights into the low 60s (or even upper 50s?). Is that okay? (I suspect it will significantly slow incubation.)

I appreciate this feedback very much.

-------

I've got practical and theoretical questions remaining.

Practical. It looks like yet another of my jars might have some darker material in it (possible mold) in the bottom. They look ready to birth and dunk.
  • should I dunk these all together - the good + the questionable - or separately?
  • are these growths the result of too high a humidity or heat during colonization?
  • can I fruit them all together? Or keep the moldy ones together in a second location, and the bacterial one in a third location?
  • should they be treated with H2O2?
  • should I cut or scrape contams off of cakes?


Theoretical. I wonder
  • whether the first jar infection above is bacterial,
  • whether the second jar infection above is mold.

in case there's any feedback about these questions out there.

Thanks!

Edited by Groovy Hobbit (12/09/15 09:12 AM)

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22633392 -

Hey man, congrats on this grow, you are doing fine despite some minor misdirection. You are correct in saying that RH (relative humidity) is not a factor during colonization.

As for colonizing temps room temperature is great. Myc will flourish in these conditions. Some people like to build fancy incubators but they don't realize that molds like trichoderma as well as bacteria flourish at slightly than higher room temp. Warmth as well as a closed enviroment with little circulation (most people use coolers or totes as incubators) creates an optimal environment for contams. IMO it has never been worth the trouble, expense, and increased risk of contams just to shave a few days off colonizing times. I simply find the warmest spot in my house and keep them there. Just be patient :thumbup: thats the most useful skill to develop in this hobby.

I will stress however that this hobby is yours to explore and everyone likes to do things differently. If you want to use an incubator, all the power to you, just dont use a heating mat. direct heat will dry out your cakes. I imagine thats whats happened to your cake with a dark spot. Most people who build incubators use an aquarium heater submersed in a pail of water so their cakes dont dry out, but I will remind you that moisture and warmth are bacteria's best friend!

To answer your other questions:

Yes, dunk together.

Impossible to tell if the growth is a result of heat or humidity, but based on what I wrote above and your setup with the heat mat and the geolite I would say there is a high chance.

Fruit them together, toss the jar with bacteria or any other cakes that look suspect.

Dont treat with H202, myc hates that stuff.

If you spot contams before or after birthing toss the cake. Contams can be cut out if they are spotted in a monotub or a cake while they are still small, IMO this often isnt worth the trouble as you are simply spreading mold spores around.

Hope this helps you. Good luck and stay motivated! You will have :mushroom2: soon.


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Edited by PsilocyBen17 (12/09/15 11:34 AM)

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Re: First mold on colonizing BRF cakes? [Re: PsilocyBen17]
    #22635618 -

Thanks, PsilocyBen17. I'm afraid there's now only one jar not showing discoloration on the bottom. Here's the look of the cakes still in the jar:



Is it possible it's bruised mycelium? (I know: I'm grasping ;-/) But if that's a bruise, I can fruit these all together.

The earlier one - isolated - is doomed, I think: Is that white mold all over it?





If so, that can't be good to breathe.

Thanks for the help, man!

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22636011 -

the one you have fruiting looks like trich to me, trich starts white before it turns green, but it could just be knotting. Wait and see. Do you have that geolite enclosed in anything?


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Re: First mold on colonizing BRF cakes? [Re: PsilocyBen17]
    #22636282 -

I have the geolite under a dome, which I lift and wave periodically during the day.

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Re: First mold on colonizing BRF cakes? [Re: Groovy Hobbit]
    #22636330 -

Cool. Post harvest pics soon dude.


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Re: First mold on colonizing BRF cakes? [Re: PsilocyBen17]
    #22636351 -

I'm encouraged that you presume there will be a harvest!

I'll dunk the cakes with blue on them with the clean one. Then, if the blue looks like bruising, I'll fruit these all together. If it looks like mold, maybe I'll try fruiting those elsewhere.

Thanks!

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