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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Galandoi on Agar?
#22382112 - 10/15/15 06:33 AM (8 years, 3 months ago) |
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Hi, This is an experiment, I have used a potato dextrose agar recipe with a small amount of fish antibiotic to start off some Galandoi. Colonisation of mycelium appears to have stopped or slowed down. Does the following picture look like the real deal?
Thanks, any feedback appreciated
-------------------- Why reinvent the wheel when someone here has already done the science?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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thats bacteria
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Well I am stumped?
This is the deal--> I got a print that was approx. 10 months old and waited for the temp to get to 18-20DegC, Which is ideal colonization temperature. I made up the first batch of potato dextrose agar with fish antibiotic to establish galandoi mycelium for microscopy purposes. Nothing happened after a month. Zippo.
Step 2. Temperature is now 21-22 Degrees two months later. I make up a second batch of agar and decide that the spores were not hydrated enough. Using some distilled and PC sterilised water, over the flow hood I make up a spore syringe with the remaining print and let it hydrate for 24 hours. I put a few drops on the new agar plates over the hood.
But after a couple of weeks, all I get it a bit of bacteria? This just doesn't make any sense? I think I have followed every rule in the book, yet nothing has come of it? Does anyone have any tips? Thanks
-------------------- Why reinvent the wheel when someone here has already done the science?
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Yerow
Stranger



Registered: 09/22/14
Posts: 1,206
Last seen: 2 years, 7 months
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Must've been your print
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Sivarted
Stranger
Registered: 08/22/15
Posts: 1,084
Last seen: 2 months, 10 days
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Re: Galandoi on Agar? [Re: Yerow]
#22387854 - 10/16/15 10:48 AM (8 years, 3 months ago) |
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Can't see your pic, but here's what my galindoi looks like (ordered syringe from a sponsor site)

Thats a week and a half after a drop of spores.
No antibiotic, it's just mea agar.
Try again, with more than one plate, to give better odds of getting a viable one.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Re: Galandoi on Agar? [Re: Sivarted]
#22387881 - 10/16/15 10:55 AM (8 years, 3 months ago) |
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this is galindoi on agar
-------------------- SPREAD THE SPORES
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Galandoi on Agar? [Re: Aero]
#22391939 - 10/17/15 05:41 AM (8 years, 3 months ago) |
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That's a nice photo of galandoi on agar. I was searching the web before and could not find one. This photo is what remains of my print. Does it look okay? For some reason the spores are all sticking together and I don't know why. Normally I do ten plates at once, as that is what you get in a disposable eBay set. Could anything happen to the print on transit, it say it is shipped from the US to Australia?
-------------------- Why reinvent the wheel when someone here has already done the science?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Shop around for plates. Sleeves of twenty are usually the cheapest.
Ur syringe might have bacteria, but it's not necessarily from transit.
If u dropped on antibiotic media and still got bacteria with no mycelium, u will most likely wanna order a new syringe
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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If the syringe gets cloudy its bacteria.
-------------------- SPREAD THE SPORES
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Quote:
AgarStudent said: For some reason the spores are all sticking together and I don't know why.
I got flakes of spores like that in the last syringe I made. I was wondering about putting a ball bearing in the syringe to help break them up.
Quote:
blindingleaf said: Shop around for plates. Sleeves of twenty are usually the cheapest.
I actually found sleeves of 10 cheapest on ebay. I think it might suit the packaging better and/or the postal service charges on long parcels. It was just under 2euro for 10 small plates including shipping. This came from Hong Kong, for me to post the same parcel to my neighbour at the cheapest possible price is 7 euro!
This is galandoi on agar with food colouring.

Edited by blackout (10/18/15 04:33 AM)
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AgarStudent
Noob


Registered: 01/25/15
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Re: Galandoi on Agar? [Re: blackout]
#22394630 - 10/17/15 06:15 PM (8 years, 3 months ago) |
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Wow that is pretty awesome. So does mycelium on agar survive okay in the post? I would have thought it was sensitive to any variation in temperature and might suddenly drop dead! Is it okay to PM members about trading?
-------------------- Why reinvent the wheel when someone here has already done the science?
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