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InvisiblePsychoslut
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Re: Cloning Pins Directly to Agar [Re: ohmatic]
    #5704131 - 06/02/06 01:25 PM (17 years, 7 months ago)

But you don't agree that this experiment wont prove the hormone theory?


I know that it wont prove anything. If he gets some good performance you can always attribute it to the fact that its a clone with good fruiting capabilities. This experiment in no way will prove that mycelium from pin clones have more pin triggering hormones or that they had an effect on the flush.

IMO the only way to do that is to figure out exactly what hormone it is, make it artificially and test it by adding it to the casing mixture.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineRogerRabbitM
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Re: Cloning Pins Directly to Agar [Re: Psychoslut]
    #5704189 - 06/02/06 01:40 PM (17 years, 7 months ago)

Glad I got you guys thinking. That's what we like to see. Of course I knew the answer before posting the original pic. You don't think I'd post my failures do you? The o'lady doesn't even get to see those! lol

Questions about hormones aside, one thing this experiment does show is the aggressive cloning quality of pins. These pins were not sterile by any means, yet they were layed on the agar and the rapidly expanding mycelium simply overran any bacteria or molds that may have been present. You wouldn't get that same performance from a large fruit that was fully mature. Of course, I would never transfer those sections to grains because of dormant contaminant spores that may be present. Instead, a small piece of mycelium from the leading edge should be transferred to a new dish, which once grown out for a few days can then inoculate up to ten quart jars, or be transferred to LC to grow out the isolated strain for even larger inoculations.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisiblePsychoslut
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704245 - 06/02/06 01:53 PM (17 years, 7 months ago)

So the experiment failed? Was there too much bacteria on the mycelium or what?

Did you ever get to the casing point or did it fail at the grain?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisibleohmatic
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704273 - 06/02/06 02:04 PM (17 years, 7 months ago)

Quote:

RogerRabbit said:
These pins were not sterile by any means, yet they were layed on the agar and the rapidly expanding mycelium simply overran any bacteria or molds that may have been present.  You wouldn't get that same performance from a large fruit that was fully mature.




this was just what i was thinking, its amazing :smile:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkillM
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704313 - 06/02/06 02:16 PM (17 years, 7 months ago)

Quote:

RogerRabbit said:

Of course I knew the answer before posting the original pic.

You don't think I'd post my failures do you?

The o'lady doesn't even get to see those! lol







:smirk:


and he sure as hell doesn't talk about them with me...

when we are out fishin!~


lolzz


tc


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineRogerRabbitM
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Re: Cloning Pins Directly to Agar [Re: ohmatic]
    #5704326 - 06/02/06 02:20 PM (17 years, 7 months ago)

The experiment did not fail. When I said I don't post failures, I was being facetious. It was inconclusive on the hormone thing. This was already an isolated strain and an excellent performer. If anything, it may have pinned a day or two sooner, but there's too many other variables involved to be able to attribute it to just the hormones.

Perhaps the most valuable lesson is to pick pins for cloning, rather than mature fruits.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisiblePsychoslut
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704337 - 06/02/06 02:24 PM (17 years, 7 months ago)

Quote:

RogerRabbit said:
The experiment did not fail. When I said I don't post failures, I was being facetious. It was inconclusive on the hormone thing. This was already an isolated strain and an excellent performer. If anything, it may have pinned a day or two sooner, but there's too many other variables involved to be able to attribute it to just the hormones.

Perhaps the most valuable lesson is to pick pins for cloning, rather than mature fruits.
RR





Does the inner tissue of a more mature specimen have less chance of having bacteria than cloning a pin or is it about the same?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisibleohmatic
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704346 - 06/02/06 02:27 PM (17 years, 7 months ago)

Quote:

RogerRabbit said:
Perhaps the most valuable lesson is to pick pins for cloning, rather than mature fruits.





i had pins that tuned into big bombs i had pins that turned into bleh.
think of the disadvantage of not knowing if itll actually produce proper fruitbodies.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibledemiu5
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Re: Cloning Pins Directly to Agar [Re: ohmatic]
    #5704405 - 06/02/06 02:47 PM (17 years, 7 months ago)

that's what I was curious about as well, ohmatic. I suppose though, when you have the time and resources for experiemtnation, there is no guesswork in the end.


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channel your inner Larry David


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InvisibleFooMan
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5704419 - 06/02/06 02:52 PM (17 years, 7 months ago)

Quote:

RogerRabbit said:
Glad I got you guys thinking.




The wheels are spinning like rims in a rap video! Awesome thread RR!


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Quick WBS Prep


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OfflineRogerRabbitM
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Re: Cloning Pins Directly to Agar [Re: ohmatic]
    #5704888 - 06/02/06 05:37 PM (17 years, 7 months ago)

Quote:

ohmatic said
i had pins that tuned into big bombs i had pins that turned into bleh.





There seems to be some mechanism that I don't fully understand yet that causes certain pins to abort or stay small, while others get all the energy. That trigger, whatever it is seems to occur later than the time I picked the above pins. I believe it originates from within the mycelial network itself rather than in individual fruits, because I've cloned a lot of pins like this and also done g2g from jars that were pinning invitro, and never had a pin fail to break down like these did back into extremely aggressive mycelium.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlineshroomballa
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5705714 - 06/02/06 10:11 PM (17 years, 7 months ago)

RR, so you took a pin from an invitro grow, and put it in a jar of grain and it colonized quickly?


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Anything I say is fictional. Duh.


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OfflineRogerRabbitM
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Re: Cloning Pins Directly to Agar [Re: shroomballa]
    #5705851 - 06/02/06 10:48 PM (17 years, 7 months ago)

Yes. They look just like the pins on the petri dishes above after four or five days. By the time the grains colonize, the pin(s) are/is completely gone.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlineshroomballa
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5706169 - 06/03/06 12:08 AM (17 years, 7 months ago)

that's very interesting. Thanks for the tip!


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Anything I say is fictional. Duh.


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Invisibledog
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Re: Cloning Pins Directly to Agar [Re: RogerRabbit]
    #5706189 - 06/03/06 12:15 AM (17 years, 7 months ago)

Fascinating thread RR. Thanks!


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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OfflineJack_Straw
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Re: Cloning Pins Directly to Agar [Re: dog]
    #5706489 - 06/03/06 01:37 AM (17 years, 7 months ago)

bookmarked for later.


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Offlineshroomballa
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Re: Cloning Pins Directly to Agar [Re: Jack_Straw]
    #5707872 - 06/03/06 03:59 PM (17 years, 7 months ago)

Would it work to place a sterile larger fruit in a grain jar? Do you think there's a limit to how mature the fruits could be? Also, i read you dont like dunking in h202, but a bleech dip sounds like a good, safe, way to go?


--------------------
Anything I say is fictional. Duh.


Edited by shroomballa (06/03/06 04:03 PM)


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OfflineRogerRabbitM
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Re: Cloning Pins Directly to Agar [Re: shroomballa] * 1
    #5708903 - 06/03/06 08:45 PM (17 years, 7 months ago)

Use pins, not mature fruits. The pins grow very aggressively to eat for lunch any contaminant spores that might be on them. a large already slowed down fruit will not.
RR


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