Citric said:

Colonized them in open light I assume?

No idea why people are still doing this.  Since I have been back to the forums I notice all new growers doing this, and also notice tons and tons more invitro pinning.

Just because invitro pin happens does not mean the uncolonized portions are contaminated.  Correct me if you think I am wrong, but colonizing jars on a open shelf introduces them to light from day one(A pinning trigger).  Also around this time of year most of us are using fans in our houses.  So not only light, but there is barely any build up of co2 in the jars due to constant fresh air flying around/over the jars.

Back when I was very active here(Around 06 or so), when everyone incubated their jars or at least colonized them in the dark, you would see invitro pinning happening a few times a month at most.  Now I see a post almost every single day.

Just because big commercial spawn labs do it does not mean it works best for us at home.  Those spawn labs are using bags which increase the heat a lot more then a jar(Increased colonization speed).  Secondly they are usually using some sort of whole grain, in which you can shake/mix the bag up to speed colonization.

If you don't want to incubate your jars in heat(Which I have no idea why you wouldn't), at the very least throw them in a rubbermaid tub.

Edit: While invitro pinning isn't detrimental to the grow, it is annoying as hell to deal with.  Having to lose substrate(Specially from a cake, it is small enough as is) is not fun and a waste of work.

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Quote:

light by itself is NOT a pinning trigger UNLESS you have full colonization and fresh air getting into the jars.  that jar pinned cuz it thought it was done colonizing. 

I and other TCs leave our jars in a light cycle for circadian rythmicity and Ive never had any prob with in-vitro pinning.

its all about timing and knowing when to trigger the myc into its natural phases.

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