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OfflineEquilibriuM
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Registered: 07/17/05
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Re: Simple Invitro Poo Bags [Re: Joops]
    #5338913 - 02/25/06 07:24 PM (17 years, 11 months ago)

awesome tek man. im doing something similar, except i'm injecting the bags with LC and not spawning to them with grain... ill know in a week or so if it will work out as well as your stuff is...


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OfflineJefferson_Lucky
Jefferson Lucky
Registered: 11/11/05
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5339045 - 02/25/06 08:05 PM (17 years, 11 months ago)

Why not resoak those blocks/bags if your not getting good second flushes?


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: Jefferson_Lucky]
    #5456350 - 03/29/06 06:39 PM (17 years, 9 months ago)

I havent even been dunking, just adding a bit of bottled water when there is none evident in the bottom, the shit logs soak it right up. LOVE IT!!!


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #5575301 - 04/30/06 08:45 PM (17 years, 8 months ago)


First Bag-No Problems

It only got better from here. GT's, Aussies, Blue Meanies, and Mexicans did great.


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Edited by Gretchenmeister (04/30/06 09:03 PM)


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OfflineNeoQ
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Registered: 04/26/06
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #5576054 - 05/01/06 12:52 AM (17 years, 8 months ago)

great post bro. We should call this Hydr0420-tek:)

Where did u get that Texas syringe?

I am a newbie but I got to ask: To spawn the jars to use in your tek, we can use the PF-tek to let the mycelium grow in the jars and use that as spawn?


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Offlinehydr0420
Psycopahtic Ryda

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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5576500 - 05/01/06 07:31 AM (17 years, 8 months ago)

Quote:

NeoQ said:
great post bro. We should call this Hydr0420-tek:)

Where did u get that Texas syringe?

I am a newbie but I got to ask:  To spawn the jars to use in your tek, we can use the PF-tek to let the mycelium grow in the jars and use that as spawn?




I got the texas from a trade. Im not sure where the original culture came from. I will try to find out.

I have heard from several people who have used pf cakes broken up to spawn the poo and it works just the same.
Good Luck :smile:


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5577225 - 05/01/06 12:48 PM (17 years, 8 months ago)

Have you tried using Tryptamine HCL to make shrooms 3 times more potent with your tek?


Here is a thread I started, its popular: Make shrooms 3 times more potent with Tryptamine HCL

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5561260/an/0/page/2


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5577234 - 05/01/06 12:51 PM (17 years, 8 months ago)

Also is the "Golden Teacher" a good strain to with this?

I only grew from a few "all ready made" mycelium I had bought. I am going to use the PF-Tek but would also like to try yours.


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5577866 - 05/01/06 04:25 PM (17 years, 8 months ago)

Quote:

NeoQ said:
Also is the "Golden Teacher" a good strain to with this?

I only grew from a few "all ready made" mycelium I had bought. I am going to use the PF-Tek but would also like to try yours.




I personally never ran GTs in them, Gretchen did with pretty good success. You will just have to try a couple different strains and then run with the one that produces best for you. Thats how I did it.


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5580176 - 05/02/06 01:42 AM (17 years, 8 months ago)

So have you added Tryptamine HCL to make shrooms more potent with your tek?


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5580508 - 05/02/06 06:43 AM (17 years, 8 months ago)

very nice.
also see cambos [invitro bags]



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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5580865 - 05/02/06 09:44 AM (17 years, 8 months ago)

Quote:

Hippie3 said:
very nice.
also see cambos [invitro bags]






Looks nice.
Afer reading his writeup over on your boards, it seems like he would do better if he got filterpatch bags, instead of fanning them daily. Especially during the colonization. If i read it right, it takes almost 2weeks for his bags to colonize, mine are usually done in 5-7.

Good grow none the less though.


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Offlinemaoru
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Registered: 02/21/06
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5592639 - 05/05/06 02:12 AM (17 years, 8 months ago)

Sweet tek man!
This was my first time grow, and it went awesomely. Well, not as good as yours - I got just over 2 oz dried from my first flush from the 5lbs tennstud. I am growing tasmanian now and next up is hawaiian.
Thanks so much for this tek! I wouldn't have even tried to grow shrooms if i hadn't seen this, because I am probably about as lazy as you, or maybe more lazy since I used duct tape instead of impulse sealing. I probably won't grow shrooms any other way, because they all take work. THANKS!


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OfflineSyriss162
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Re: Simple Invitro Poo Bags [Re: maoru]
    #5593397 - 05/05/06 09:59 AM (17 years, 8 months ago)

Very interesting tek, I'm planning on giving it a go after my LC is finished and i colonize some wbs. Its not that im lazy I just have plenty of other things to take care of everyday and this way i wont have to constantly be checking rh and whatnot. Planning on making 6-10 2lb bags, half B+ and half GT, and I'll tell you how it turns out.


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5601487 - 05/07/06 03:43 PM (17 years, 8 months ago)

i just re-read the thread
and he wasn't explicit as to
how long his spawn run took,
just that he began fanning
at the start of week 2.
presumbly that means it's finished, like yours,
in about a week.
but i do agree that filter patches would be improvement.
but perhaps even larger patches than what one gets
on commercial pre-made filter bags.
see maximized invitro bag tek





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Offlinehydr0420
Psycopahtic Ryda

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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5601936 - 05/07/06 05:52 PM (17 years, 8 months ago)

Quote:

Hippie3 said:
i just re-read the thread
and he wasn't explicit as to
how long his spawn run took,
just that he began fanning
at the start of week 2.
presumbly that means it's finished, like yours,
in about a week.
but i do agree that filter patches would be improvement.
but perhaps even larger patches than what one gets
on commercial pre-made filter bags.
see maximized invitro bag tek








Interesting. Ive never really visited your site, but his tek seems to be quite long winded, Im sure its written well, I dont have that kind of patience. But it looks like he is doing alot of sterilizing in there, and whatnot.
The reason I did mine the way I did, is because I am too lazy to do all that shit. The only thing I have to sterilize was the WBS I used to spawn. Everything else I purchased ready to go, to minimize my effort put into it. As my title reads "SIMPLE Invitro Poo Bags".
Each bag when said and done cost around 3$ of consumable supplies.
These bags worked awesome for me when I did them, Ive since stopped growing, but each 2lb bag averaged 2oz dry over a couple flushes. First flush alone was usually 3-400g wet.


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5664321 - 05/23/06 12:59 PM (17 years, 8 months ago)

http://gretchensecret.photosite.com/G-bags5232006/

less than five days after spawning with ultra pulverized pf cakes (cambodian long stem)...full colonization and primordia. Love it!


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What's wrong with folks?

Point your IRC client to irc.socialirc.com, port 6667, #cultivation and #shroomery for live chat with like minded hobbyists and connoisseurs.


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5664707 - 05/23/06 02:12 PM (17 years, 8 months ago)

Quote:

hydr0420 said:, but his tek seems to be quite long winded, Im sure its written well, I dont have that kind of patience. But it looks like he is doing alot of sterilizing in there, and whatnot...




not really,
just the spawn jars [wbs or brf],
the rest is non-sterile procedure

Quote:

1. Prepare & pressure cook numerous 500ml wbs jars.
2. Inoculate above jars. Steps 3 & 4 can be done during germination & colonization.
3. Prep & PC legions of wbs jars.
4. Prepare double-layer tyvek breathing patches. Tape onto oven bags & PC oven bags 30 min.
5. Spawn wbs from colonized jars made in step 1 with jars of step 3.
6. Prep & PC jars of wetted vermiculite. Ratio of verm jars to wbs is 6:8.
7. Take 8 colonized jars from step 5 and 6 verm jars from step 6 and crumble/empty into a bag from step 4. Tie shut, mix bag & place on shelf until harvest time.
8. After harvest, crumble mass into large bowl. Add ½ ~ 2/3 of water lost to flush & mix. Load into new bag, tie shit & place on shelf again. Repeat until flushes are no longer worth the shelf space.







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OfflineMeckaDa
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Registered: 12/09/05
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5664830 - 05/23/06 02:47 PM (17 years, 8 months ago)

sorry to interrupt, i just got one or two questions.
so here is the thing. i have a full colonized mono-tub(hpoo). not yet with holes. here is some pics



See the white knots. or is it small primadorias?
now i wonder if i could take the colonized hpoo and put it on a spawn-bag and colonizes for 3-4 days more. then just fruit like the "Simple Invitro Poo Bags" does..
would the knots/pins do any harm when i transfer to the spawn-bag? what can i do...
and no i can't just continue in the mono-tub. because my fantasy grow-space is limited. just can use spawn-bags.
thanks  :mushroom2:


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #5665014 - 05/23/06 03:18 PM (17 years, 8 months ago)

Breaking that up now would fuck it up IME, I'd leave it.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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