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Offlinehydr0420
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Simple Invitro Poo Bags * 1
    #5278987 - 02/08/06 07:38 PM (17 years, 11 months ago)

Simple, Lazy, Invitro Mushroom Bags

Okay, I'm lazy, I'll admit it.
I've been growing mushrooms for about 2 years now, I've done pf cakes in PoorMans Pod Terrariums, then I moved to rye casings in the same terrariums, then I did large cased tubs of rye. I've done Martha closets with casings, and even with spawn bags that I just cut the tops off of.
I'm too lazy for all that shit, have to make sure the humidifiers have water, making sure the air stones for the PMPs are working, fan the tubs, I didn't even like making up casings.
I prefer to set it and forget it.
So, after much testing, I came up with this idea to use pasteurized poo (Pre-Pasteurized from tennstudd, so I don't have to do any work to it, just open the box and spawn the shit), put that in filterpatch bags, which provide air exchange and keep in humidity.
Leave the bags in the dark for a week or so to colonize, then put them in light for another week and come back and pick mushrooms.
No misting, No fanning, and the lights in the closet are on a timer, so I don't even have to mess with that.


Materials needed:
1) Colonized jars of spawn - I use Pint jars of colonized WBS for my spawn jars. You could use colonized rye jars, or even shredded pf jars I think would work just fine.


2) Large empty filterpatch spawn bags - I purchase these from either mycofactory.com or sporeworks.com.


3) Pre-Pasteurized 50/50 (poo/straw) mix from tennstud.com


4) Reynolds Turkey Size Oven bags - These are used to evenly mix the spawn and the poo before putting it into single bags.



DIRECTIONS

Prepare and colonize your spawn jars as you normally would for any growing.
Take your colonized jars of spawn.
Open your bag of tennstudd poo. (The poo usually comes in 5lb bags)
I use 1pint of spawn per lb of poo. (so 5pints of spawn per 5lb bag from tennstud)
Pour the poo into your Reynolds oven bag.
Put in your spawn jars.

Break up all the spawn.

Mix it all around really well.

I pour 2lbs of this mix into each filterpatch bag, then impulse seal it. (I don't think that impulse sealing these bags is necessary since we are not working with sterile substrate, I think you could get away with folding over the top really well then putting clothespins on the top.)


Each 5lb bag of tennstudd poo + spawn mixed will make 4 bags or so.
These bags are then placed in a dark closet at room temperature to colonize (70-75 degrees)


They usually take 7-10 days to colonize, they then are thrown in a closet with light to fruit.


A week or so later, you get pins and mushrooms.





Once the shrooms mature, I take the bags outta the closet, cut off the tops, harvest, spray the inside with water/h2o2 mix, then put a clothespin on and wait for the next flush. Subsequent flushes on these bags are not too great, but the first flush usually gives 300-600g of wet fresh mushrooms. I use PF Classic, Texas, Hawaiian strains in the bags. I have tried others that did not perform as well as these 3 have, your results may vary.








There, simple mushrooms, lets see what comments this gets.
-dr0


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Offlineonetime
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5279005 - 02/08/06 07:41 PM (17 years, 11 months ago)

sweet I suposse. It aways seemed to me that useing bags to fruit is a bit limited in the amount of space you have for the shrooms to grow. I think that are great for spawn fruiting seemed avgerage to me up untill I seen your pics. Nice job man.


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5279026 - 02/08/06 07:44 PM (17 years, 11 months ago)

Excellent. This looks simple and efficient. Thanks for taking the time put this together...not alot of bag-tek info out there.


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OfflinerxKaffee
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #5279038 - 02/08/06 07:48 PM (17 years, 11 months ago)

HELL YES! thnx thnx thnx.

hom0420 : you are the man!


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Offlinestelthvue
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Re: Simple Invitro Poo Bags [Re: rxKaffee]
    #5279058 - 02/08/06 07:52 PM (17 years, 11 months ago)

that is sweet. thanks for clearing up whether or not you have to PC tennstud poo. sweet tek.


--------------------
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OfflineFGL
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Re: Simple Invitro Poo Bags [Re: stelthvue]
    #5279086 - 02/08/06 07:59 PM (17 years, 11 months ago)

:thumbup:


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Offlinedown44
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Re: Simple Invitro Poo Bags [Re: FGL]
    #5279255 - 02/08/06 08:52 PM (17 years, 11 months ago)

awesome tek cant wait to stank on it


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Offlineblackout
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Re: Simple Invitro Poo Bags [Re: down44]
    #5280507 - 02/09/06 02:17 AM (17 years, 11 months ago)

Great work!


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Offline_OttO_
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Re: Simple Invitro Poo Bags [Re: blackout]
    #5280893 - 02/09/06 08:09 AM (17 years, 11 months ago)

Looks good - they end up a bit tangled but who cares especially if you grind them anyway. Nice simple tek.


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Invisibleohmatic
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Re: Simple Invitro Poo Bags [Re: _OttO_]
    #5280901 - 02/09/06 08:17 AM (17 years, 11 months ago)

so our little #cultivation monkey finally did a writeup :wink:
nice work there :smile:
peace ohm :mushroom2:


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OfflinerDr4g0n
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Re: Simple Invitro Poo Bags [Re: ohmatic]
    #5280931 - 02/09/06 08:29 AM (17 years, 11 months ago)

all this time we been makin gloveboxes and flowhoods and marthas when all we need is walmart bags and some poo and a few days. pshaw lol


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Invisibleohmatic
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Re: Simple Invitro Poo Bags [Re: rDr4g0n]
    #5280940 - 02/09/06 08:35 AM (17 years, 11 months ago)

Quote:

rDr4g0n said:
all this time we been makin gloveboxes and flowhoods and marthas when all we need is walmart bags and some poo and a few days. pshaw lol




just think of what you CANT do without gloveboxes, flowhoods, marthas.

the bags are just a quick and dirty solution.
peace ohm :mushroom2:


--------------------
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InvisibleNormlizer420
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Re: Simple Invitro Poo Bags [Re: ohmatic]
    #5282771 - 02/09/06 05:59 PM (17 years, 11 months ago)

wow nice tek :smile: One question around how many flushes are them bags good for?


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InvisibleFooMan
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Re: Simple Invitro Poo Bags [Re: Normlizer420]
    #5282809 - 02/09/06 06:13 PM (17 years, 11 months ago)

Off topic, but do you have any of those Pan Cebu prints or syringes for trade? Shoot me a PM if you do. Thanks


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Normlizer420]
    #5283655 - 02/09/06 09:30 PM (17 years, 11 months ago)

They give out flushes untill they completely die. If you dunk after each flush you get more flushes 5-6. The later flushes usually are just a couple shrooms, but if you have the space to just leave the bags on a shelf, its not hurting anything or any extra work to just pick a couple shrooms now and then. And since they are in their own enviroment, if they end up contaming after a while, its contained to the one bag.


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Offline_OttO_
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Re: Simple Invitro Poo Bags [Re: FooMan]
    #5284709 - 02/10/06 05:59 AM (17 years, 11 months ago)

Quote:

FooManShroom said:
Off topic, but do you have any of those Pan Cebu prints or syringes for trade? Shoot me a PM if you do. Thanks




What the hell is Pan Cebu??


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Offlineblackout
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Re: Simple Invitro Poo Bags [Re: _OttO_]
    #5284732 - 02/10/06 06:08 AM (17 years, 11 months ago)

pan cyans from the phillipines I think


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Offline_OttO_
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Re: Simple Invitro Poo Bags [Re: blackout]
    #5284885 - 02/10/06 08:09 AM (17 years, 11 months ago)

Cool, thanx Blackout. *end of thread hijack*


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Offlineg00n
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5296867 - 02/13/06 08:41 PM (17 years, 11 months ago)

This looks great... can't wait to try it. Thanks hydr0420


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OfflineJoops
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Re: Simple Invitro Poo Bags [Re: g00n]
    #5338736 - 02/25/06 06:26 PM (17 years, 11 months ago)

Got 30lbs of pre-past tennstud on the way, along with 25 large grow bags and a 12" impulse sealer. Total about $130 shipped (tennstudd is $32 shipping alone due to lcoation). According to hydro it should make 24 2lb bags.

Hopefully it works out!


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OfflineEquilibriuM
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Re: Simple Invitro Poo Bags [Re: Joops]
    #5338913 - 02/25/06 07:24 PM (17 years, 11 months ago)

awesome tek man. im doing something similar, except i'm injecting the bags with LC and not spawning to them with grain... ill know in a week or so if it will work out as well as your stuff is...


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OfflineJefferson_Lucky
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5339045 - 02/25/06 08:05 PM (17 years, 11 months ago)

Why not resoak those blocks/bags if your not getting good second flushes?


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: Jefferson_Lucky]
    #5456350 - 03/29/06 06:39 PM (17 years, 9 months ago)

I havent even been dunking, just adding a bit of bottled water when there is none evident in the bottom, the shit logs soak it right up. LOVE IT!!!


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InvisibleGretchenmeister
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #5575301 - 04/30/06 08:45 PM (17 years, 8 months ago)


First Bag-No Problems

It only got better from here. GT's, Aussies, Blue Meanies, and Mexicans did great.


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Edited by Gretchenmeister (04/30/06 09:03 PM)


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #5576054 - 05/01/06 12:52 AM (17 years, 8 months ago)

great post bro. We should call this Hydr0420-tek:)

Where did u get that Texas syringe?

I am a newbie but I got to ask: To spawn the jars to use in your tek, we can use the PF-tek to let the mycelium grow in the jars and use that as spawn?


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5576500 - 05/01/06 07:31 AM (17 years, 8 months ago)

Quote:

NeoQ said:
great post bro. We should call this Hydr0420-tek:)

Where did u get that Texas syringe?

I am a newbie but I got to ask:  To spawn the jars to use in your tek, we can use the PF-tek to let the mycelium grow in the jars and use that as spawn?




I got the texas from a trade. Im not sure where the original culture came from. I will try to find out.

I have heard from several people who have used pf cakes broken up to spawn the poo and it works just the same.
Good Luck :smile:


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5577225 - 05/01/06 12:48 PM (17 years, 8 months ago)

Have you tried using Tryptamine HCL to make shrooms 3 times more potent with your tek?


Here is a thread I started, its popular: Make shrooms 3 times more potent with Tryptamine HCL

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5561260/an/0/page/2


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5577234 - 05/01/06 12:51 PM (17 years, 8 months ago)

Also is the "Golden Teacher" a good strain to with this?

I only grew from a few "all ready made" mycelium I had bought. I am going to use the PF-Tek but would also like to try yours.


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #5577866 - 05/01/06 04:25 PM (17 years, 8 months ago)

Quote:

NeoQ said:
Also is the "Golden Teacher" a good strain to with this?

I only grew from a few "all ready made" mycelium I had bought. I am going to use the PF-Tek but would also like to try yours.




I personally never ran GTs in them, Gretchen did with pretty good success. You will just have to try a couple different strains and then run with the one that produces best for you. Thats how I did it.


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OfflineNeoQ
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5580176 - 05/02/06 01:42 AM (17 years, 8 months ago)

So have you added Tryptamine HCL to make shrooms more potent with your tek?


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5580508 - 05/02/06 06:43 AM (17 years, 8 months ago)

very nice.
also see cambos [invitro bags]



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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5580865 - 05/02/06 09:44 AM (17 years, 8 months ago)

Quote:

Hippie3 said:
very nice.
also see cambos [invitro bags]






Looks nice.
Afer reading his writeup over on your boards, it seems like he would do better if he got filterpatch bags, instead of fanning them daily. Especially during the colonization. If i read it right, it takes almost 2weeks for his bags to colonize, mine are usually done in 5-7.

Good grow none the less though.


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Offlinemaoru
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5592639 - 05/05/06 02:12 AM (17 years, 8 months ago)

Sweet tek man!
This was my first time grow, and it went awesomely. Well, not as good as yours - I got just over 2 oz dried from my first flush from the 5lbs tennstud. I am growing tasmanian now and next up is hawaiian.
Thanks so much for this tek! I wouldn't have even tried to grow shrooms if i hadn't seen this, because I am probably about as lazy as you, or maybe more lazy since I used duct tape instead of impulse sealing. I probably won't grow shrooms any other way, because they all take work. THANKS!


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OfflineSyriss162
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Re: Simple Invitro Poo Bags [Re: maoru]
    #5593397 - 05/05/06 09:59 AM (17 years, 8 months ago)

Very interesting tek, I'm planning on giving it a go after my LC is finished and i colonize some wbs. Its not that im lazy I just have plenty of other things to take care of everyday and this way i wont have to constantly be checking rh and whatnot. Planning on making 6-10 2lb bags, half B+ and half GT, and I'll tell you how it turns out.


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5601487 - 05/07/06 03:43 PM (17 years, 8 months ago)

i just re-read the thread
and he wasn't explicit as to
how long his spawn run took,
just that he began fanning
at the start of week 2.
presumbly that means it's finished, like yours,
in about a week.
but i do agree that filter patches would be improvement.
but perhaps even larger patches than what one gets
on commercial pre-made filter bags.
see maximized invitro bag tek





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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5601936 - 05/07/06 05:52 PM (17 years, 8 months ago)

Quote:

Hippie3 said:
i just re-read the thread
and he wasn't explicit as to
how long his spawn run took,
just that he began fanning
at the start of week 2.
presumbly that means it's finished, like yours,
in about a week.
but i do agree that filter patches would be improvement.
but perhaps even larger patches than what one gets
on commercial pre-made filter bags.
see maximized invitro bag tek








Interesting. Ive never really visited your site, but his tek seems to be quite long winded, Im sure its written well, I dont have that kind of patience. But it looks like he is doing alot of sterilizing in there, and whatnot.
The reason I did mine the way I did, is because I am too lazy to do all that shit. The only thing I have to sterilize was the WBS I used to spawn. Everything else I purchased ready to go, to minimize my effort put into it. As my title reads "SIMPLE Invitro Poo Bags".
Each bag when said and done cost around 3$ of consumable supplies.
These bags worked awesome for me when I did them, Ive since stopped growing, but each 2lb bag averaged 2oz dry over a couple flushes. First flush alone was usually 3-400g wet.


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5664321 - 05/23/06 12:59 PM (17 years, 8 months ago)

http://gretchensecret.photosite.com/G-bags5232006/

less than five days after spawning with ultra pulverized pf cakes (cambodian long stem)...full colonization and primordia. Love it!


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Point your IRC client to irc.socialirc.com, port 6667, #cultivation and #shroomery for live chat with like minded hobbyists and connoisseurs.


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InvisibleHippie3
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #5664707 - 05/23/06 02:12 PM (17 years, 8 months ago)

Quote:

hydr0420 said:, but his tek seems to be quite long winded, Im sure its written well, I dont have that kind of patience. But it looks like he is doing alot of sterilizing in there, and whatnot...




not really,
just the spawn jars [wbs or brf],
the rest is non-sterile procedure

Quote:

1. Prepare & pressure cook numerous 500ml wbs jars.
2. Inoculate above jars. Steps 3 & 4 can be done during germination & colonization.
3. Prep & PC legions of wbs jars.
4. Prepare double-layer tyvek breathing patches. Tape onto oven bags & PC oven bags 30 min.
5. Spawn wbs from colonized jars made in step 1 with jars of step 3.
6. Prep & PC jars of wetted vermiculite. Ratio of verm jars to wbs is 6:8.
7. Take 8 colonized jars from step 5 and 6 verm jars from step 6 and crumble/empty into a bag from step 4. Tie shut, mix bag & place on shelf until harvest time.
8. After harvest, crumble mass into large bowl. Add ½ ~ 2/3 of water lost to flush & mix. Load into new bag, tie shit & place on shelf again. Repeat until flushes are no longer worth the shelf space.







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OfflineMeckaDa
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5664830 - 05/23/06 02:47 PM (17 years, 8 months ago)

sorry to interrupt, i just got one or two questions.
so here is the thing. i have a full colonized mono-tub(hpoo). not yet with holes. here is some pics



See the white knots. or is it small primadorias?
now i wonder if i could take the colonized hpoo and put it on a spawn-bag and colonizes for 3-4 days more. then just fruit like the "Simple Invitro Poo Bags" does..
would the knots/pins do any harm when i transfer to the spawn-bag? what can i do...
and no i can't just continue in the mono-tub. because my fantasy grow-space is limited. just can use spawn-bags.
thanks  :mushroom2:


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Offlinescatmanrav
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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #5665014 - 05/23/06 03:18 PM (17 years, 8 months ago)

Breaking that up now would fuck it up IME, I'd leave it.


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OfflineMeckaDa
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Re: Simple Invitro Poo Bags [Re: scatmanrav]
    #5665047 - 05/23/06 03:24 PM (17 years, 8 months ago)

i have read that the small pins can rotten inside a casing if it's not removed. have i the same problem or could i just pick away the pins before i put it in spawn-bags?


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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #5665350 - 05/23/06 04:15 PM (17 years, 8 months ago)

if you must
then you must.
but i wouldn't crumble it,
i'd just cut it into a section that will fit in the bag
and transfer whole.


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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5665434 - 05/23/06 04:35 PM (17 years, 8 months ago)

as nice as it is, aesthetically casings still win :wink:


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Re: Simple Invitro Poo Bags [Re: ohmatic]
    #5667300 - 05/24/06 12:40 AM (17 years, 8 months ago)

alright, I've change my mind. but do you think it's to late for the a casing layer? this morning i saw knots all over. thanks guys


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Re: Simple Invitro Poo Bags [Re: ohmatic]
    #5667712 - 05/24/06 06:23 AM (17 years, 8 months ago)

Quote:

ohmatic said:
as nice as it is, aesthetically casings still win :wink:




agreed
but one doesn't trip off 'aesthetics' eh ?


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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #5667716 - 05/24/06 06:24 AM (17 years, 8 months ago)

you could apply a thin [1/4 inch] casing even now
and still get some benefit
esp. for 2nd flush


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Offlinezeegos
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5667730 - 05/24/06 06:31 AM (17 years, 8 months ago)

For hpoo substrate and casings, does fresher meen better?
I have stables close by so i might do a night mission to get myself some. Funny if someone found me at night stealing horse dung from a stable  :grin:


Edited by zeegos (05/24/06 06:41 AM)


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Re: Simple Invitro Poo Bags [Re: zeegos]
    #5667804 - 05/24/06 07:36 AM (17 years, 8 months ago)

no, fresh horse manure is 'hot', too much nitrogen.
it must be aged, composted, dried before use.
you'd want the old manure that's been cleaned out of the stable
a couple months ago.


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Offlinezeegos
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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #5667838 - 05/24/06 08:02 AM (17 years, 8 months ago)

There is plenty of hpoo in fields which is just dry clumps. Would this be useless? Im not sure if the rain would have washed out all the nutes.


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Re: Simple Invitro Poo Bags [Re: zeegos]
    #5667892 - 05/24/06 08:46 AM (17 years, 8 months ago)

Quote:

zeegos said:
There is plenty of hpoo in fields which is just dry clumps. Would this be useless? Im not sure if the rain would have washed out all the nutes.




this would be just what your looking for.


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Re: Simple Invitro Poo Bags [Re: ohmatic]
    #5668687 - 05/24/06 12:48 PM (17 years, 8 months ago)

pretty sweet! the only reason i didn't try this is because of the fruits being twisted up inside the bag...

now i don't care! will be doing this soon, just ordered 20lbs. tnn stud. *Wahoo*


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Re: Simple Invitro Poo Bags [Re: Oatman2000]
    #5794922 - 06/27/06 04:43 AM (17 years, 7 months ago)

Any updates all?


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Re: Simple Invitro Poo Bags [Re: Capless]
    #5796849 - 06/27/06 06:59 PM (17 years, 7 months ago)

I'm gonna give this a shot,Just knocked up some wbs the other day and the Tenn studd is already here.I will let ya know how it goes !!


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #5891800 - 07/23/06 06:32 PM (17 years, 6 months ago)

AFOAF tried this tek with some GT. spawn was a 1lb mycobag (brf) mixed with 5lbs of tennstud. he says the bags are exploding with pins. he just wanted everyone to know that he had success with GT and this tek.


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Re: Simple Invitro Poo Bags [Re: ackmess]
    #5941035 - 08/07/06 02:54 PM (17 years, 5 months ago)

great ackmess! now we know, any other strains people have good results with, pics maybe?

:wink:


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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #5984118 - 08/21/06 03:28 PM (17 years, 5 months ago)

anyone???


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Re: Simple Invitro Poo Bags [Re: MeckaDa]
    #6045720 - 09/10/06 01:21 AM (17 years, 4 months ago)

I made some bags but instead of poo i'm using coco coir + coffee grounds + calcium carbonate. It's full colonized and some knots are appearing inside, A LOT of them. Pins are coming.


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Re: Simple Invitro Poo Bags [Re: shroower]
    #6046594 - 09/10/06 12:47 PM (17 years, 4 months ago)

Ive been using this for a bit now since I saw this thread, I love it personally. If a bag contams, its sealed, you toss it. I take 10 pounds wet poo, split into 8 filter patch bags, dump a pint of spawn into each one, mix, seal, and let them go. Easy as hell, 8 pints of spawn, 8 bags and 10 pounds wet poo and you got a nice little batch with little time.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Re: Simple Invitro Poo Bags [Re: scatmanrav]
    #6046803 - 09/10/06 02:22 PM (17 years, 4 months ago)

Would it be alright just to inject LC into the poo/straw right in the grow bag? Would you get the same results because that would make the process so much easier and i would be making 100's.

Also whenever they start to pint or straight to actually fruit would it ok to cut them out and put some in a PMP for more growth?

Man my mind is a working here...i see bright things in my future  :smile:


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Edited by bigshroom (09/10/06 02:31 PM)


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Re: Simple Invitro Poo Bags [Re: bigshroom]
    #6047169 - 09/10/06 04:08 PM (17 years, 4 months ago)

Im thinking this might be the best bet for me also since i need to be a little more on the stealthy side!I was wondering how sterile do you need to be doing this?What i mean is did you use a flowhood(dont see one but jsut asking? Or just a good whipe down with alcohol , some oust, gloves ,face mask etc. when you were mixing the poo and spawn jars!

cheers,
Outer


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Re: Simple Invitro Poo Bags [Re: Outer]
    #6105398 - 09/26/06 10:04 PM (17 years, 4 months ago)

Damn kids. I havent been on the boards much in the past couple months, im suprised to see this thread still kicking around. I may just have to pay more attention to this shit, to help answer questions for people trying this.

Scatmanrav; Im flattered you are trying this method, I was always impressed with your other grows. PM me, I would like to hear your experiences with it.

Im glad bags are catching on.


Quote:

Outer said:
Im thinking this might be the best bet for me also since i need to be a little more on the stealthy side!I was wondering how sterile do you need to be doing this?What i mean is did you use a flowhood(dont see one but jsut asking? Or just a good whipe down with alcohol , some oust, gloves ,face mask etc. when you were mixing the poo and spawn jars!

cheers,
Outer




Honestly the only time you need to be sterile is when you make your spawn jars. Once you are working with pasteurized poo, you dont have to worry as much.
I wiped down the bathroom with bleach, ran a cheapass walmart HEPA filter in there for an hour, then did everything OPEN AIR. Half the time I didnt even wash my hands. You may not have as good of results. But when you are working with pasteurized substrates its not sterile, so you dont have to be, just be clean and careful.


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Offlinebw86
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6275548 - 11/12/06 02:04 PM (17 years, 2 months ago)

I have a couple questions.

After letting them colonize in the dark I need to place them in a closet with light? Should I let the light beat down directly on the bags or should they be out of direct light? Do you leave the closet door open or closed. Also it says that the lights are on timers - what are good settings to use? Would intense blue leds help at growth at all?


Edited by bw86 (11/12/06 02:39 PM)


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: bw86]
    #6275850 - 11/12/06 03:47 PM (17 years, 2 months ago)

I have Q's too, it looks like you have the bags in a martha to fruit.. I briefly glanced at the tek but I thought you said they kept humidity in very well... Could you just fruit them on a shelf in open air somewhere or would they need to be in a humid environment... Also, is the sealer part of a vacuum sealer (foodsaver) an impulse sealer? I obviously wouldn't use the vaccum part of it just the seal feature.... an where can I get those bags??



NICE FUCKING WRITE UP tho! If you can answer those questions for me I'll be trying it for sure! I like your style...


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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6275864 - 11/12/06 03:51 PM (17 years, 2 months ago)

get a 12 inch impulse sealer off of ebay headie...unless you already have a food saver with a sealer...

and you can leave the bags on a shelf....there is no need for external humidity

and you can get the bags from www.sporeworks.com


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Re: Simple Invitro Poo Bags [Re: BIGSWANG]
    #6275914 - 11/12/06 04:01 PM (17 years, 2 months ago)

thanks dawg why doesn't everyone grow this way...!!?!?!

I know why, I'm just a not a perfectionist like most everyone else...

Thanks BIGSWANG AND DRO!


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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6276201 - 11/12/06 05:28 PM (17 years, 2 months ago)

I did this exact same thing a while back,out of 17 bags 4 of them fruited and even that was after waiting so long that I cut the tops off and started fanning,because nothing useful will get through the filter patch,O and it wasn't like the other ons contaminated or anything,they just stalled and took so long to pin that they gave up and didn't even try,I thought this would have been a nice painless way to grow,and it would be if I had better results,It is a good tek but you will have to be very patient and wait forever to see anything,the ones that did fruit were colonized for a month before I saw pins


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Re: Simple Invitro Poo Bags [Re: rxKaffee]
    #6276338 - 11/12/06 06:00 PM (17 years, 2 months ago)

Quote:

rxKaffee said:
HELL YES! thnx thnx thnx.

hom0420 : you are the man!




Hey rxKaffee, its Hydr0420 not hom0420....


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6276875 - 11/12/06 08:37 PM (17 years, 2 months ago)

Quote:

headieherbs said:
I have Q's too, it looks like you have the bags in a martha to fruit.. I briefly glanced at the tek but I thought you said they kept humidity in very well... Could you just fruit them on a shelf in open air somewhere or would they need to be in a humid environment... Also, is the sealer part of a vacuum sealer (foodsaver) an impulse sealer? I obviously wouldn't use the vaccum part of it just the seal feature.... an where can I get those bags??



NICE FUCKING WRITE UP tho! If you can answer those questions for me I'll be trying it for sure! I like your style...




Wow, this thread is still alive.

I know this has already been answered, but.
The shelves the bags are on are not in a martha, they are cheap plastic shelves from target. Its like 25$ for a 4 shelf setup that can fit 48bags.
The sealer is just a normal Impulse Sealer which can be had from eBay or search google for impulse sealer. Theres a company called Cleveland something or other, which has some of the best impulse sealer prices.

Quote:

Blutjager said:
I did this exact same thing a while back,out of 17 bags 4 of them fruited and even that was after waiting so long that I cut the tops off and started fanning,because nothing useful will get through the filter patch,O and it wasn't like the other ons contaminated or anything,they just stalled and took so long to pin that they gave up and didn't even try,I thought this would have been a nice painless way to grow,and it would be if I had better results,It is a good tek but you will have to be very patient and wait forever to see anything,the ones that did fruit were colonized for a month before I saw pins




This isnt always true. Some strains/substrains just do better in the bag conditions. I have tried strains like EQ that did shit, but every other strain did fine for me. Your results may vary, just like with any other method.

I have done sets of 80+ bags in the past with the exact same cultured spawn jars, all made at the same time in the same conditions, and 2 got green, and 4 never fruited. Its fucking random and weird, there is no reason for that, since the bags were made in 8bag batches from the same spawn jars. Shouldnt all 8 made from the same jar/poo mixed at the same time contamd? or stalled?

Mushrooms when done from multispore are gonna be random. Myc is gonna be random sometimes.

This will work for some people, not for all.


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6276985 - 11/12/06 09:09 PM (17 years, 2 months ago)

Yea,I do think it has something to do with strain,The only ones that did fruit at all were Texas(Of coarse,they even fruit in jars if ya leave them alone)Even some of them didn't work however,The treasure coast did miserable and so did the mexicub and Ecuador.O well I guess you just gotta take your chances if you want to set it and forget it


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #6277008 - 11/12/06 09:13 PM (17 years, 2 months ago)

Quote:

Blutjager said:
Yea,I do think it has something to do with strain,The only ones that did fruit at all were Texas(Of coarse,they even fruit in jars if ya leave them alone)Even some of them didn't work however,The treasure coast did miserable and so did the mexicub and Ecuador.O well I guess you just gotta take your chances if you want to set it and forget it




Meh,
You should have got more yeild, everyone else I have talked to who has tried it has had decent results. What were the closet conditions?


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Offlineautographguys
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6277602 - 11/12/06 11:58 PM (17 years, 2 months ago)

I think a tub on tub would produce way more. Bags wont get enough fae


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: autographguys]
    #6277624 - 11/13/06 12:05 AM (17 years, 2 months ago)

dro.... I have several isolation prints of the hawaiian strain... how have they performed for you with this setup... I'm strongly considering trying this method before I get into a more control oriented grow, cause of lack of funds..... I love a lazyman's view, cause I'm a LAZY MAN.... input appreciated!


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Re: Simple Invitro Poo Bags [Re: autographguys]
    #6280623 - 11/13/06 09:52 PM (17 years, 2 months ago)

Quote:

autographguys said:
I think a tub on tub would produce way more. Bags wont get enough fae




Yeah, as you can tell from the pics in the first post this method sucks, and there isnt much FAE. I think you should go post in threads about tubs instead if you dont like my method, its not for you.
You should also probably e-mail mycobag to let them know to stop selling their bags that they say you can fruit directly in the bag. Since it doesnt work.

Quote:

headieherbs said:
dro.... I have several isolation prints of the hawaiian strain... how have they performed for you with this setup... I'm strongly considering trying this method before I get into a more control oriented grow, cause of lack of funds..... I love a lazyman's view, cause I'm a LAZY MAN.... input appreciated!




I have used a HAW syringe before, it worked very well. Yours will be different from mine, but it should work. Like I said before, not every strain works in bags, just try it out and see if you like it or not.


Edited by hydr0420 (11/13/06 09:55 PM)


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6280694 - 11/13/06 10:18 PM (17 years, 2 months ago)

how bad did eq do? cause i was gonna unload my jars soon, would i be better off fruiting outside the bag on those? and i also have b+ and pink buf jars getting ready, which is the best for leaving them in the bags? i think im gonna move this soon. nice tek though hydro.


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OfflineMisterMudd
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Re: Simple Invitro Poo Bags [Re: oso]
    #6280718 - 11/13/06 10:27 PM (17 years, 2 months ago)

Can you remove the bricks from the bags and place them into a FC terrarium and fruit them like cakes? The only reason I think I would do that is to allow more room for the little guys to stretch out.


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Re: Simple Invitro Poo Bags [Re: MisterMudd]
    #6280734 - 11/13/06 10:33 PM (17 years, 2 months ago)

yea, i was thinking about that for the eq after reading it didnt do well in bags


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: MisterMudd]
    #6280756 - 11/13/06 10:38 PM (17 years, 2 months ago)

Quote:

MisterMudd said:
Can you remove the bricks from the bags and place them into a FC terrarium and fruit them like cakes? The only reason I think I would do that is to allow more room for the little guys to stretch out.




Sure, but that kinda defeats the whole purpose of doing bags invitro. You might as well just make normal casings or tubs with the same poo. The whole reason I did the bags is because once you make them, you dont have to do anything but occasionally monitor temps, and flip a lightswitch for a couple weeks, then pick.
Them stretching out doesnt matter in my experience, they keep growing even when they hit the top of the bag, usually curl back down.


Both of those were grown in bags.

Quote:

oso said:
how bad did eq do? cause i was gonna unload my jars soon, would i be better off fruiting outside the bag on those? and i also have b+ and pink buf jars getting ready, which is the best for leaving them in the bags? i think im gonna move this soon. nice tek though hydro.




EQ did total shit for me, but I am not saying it wont always work. Your multispore inoc may do better than mine, I only tried it once in bags with I think 10 bags, but in all honesty, the eq never did well for me in casings in a martha, or in casings in a PMP, my syringe may have just been shitty.
Give it a shot, theres only one way to tell if its going to work for you.


Edited by hydr0420 (11/13/06 10:39 PM)


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6323490 - 12/01/06 07:21 AM (17 years, 2 months ago)

hey hydro... sorry to reawaken this thread but would straight poo work as well as the 50-50 poo/straw mix? I ordered some pre-pasteurized from tennstud but it's straight poo.... someone please let me know quick I just ordered it a few days ago so I could probably change my order..... on another note, Ihaven't heard anythign from mike, how long does it take the shit to come?


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6481499 - 01/21/07 01:04 AM (17 years, 12 days ago)

i was recommended this method by gretchen and i am interested in using it as my first attempt at growing. a few q's though:

1)it is not necessary to add water to the bags for the first flush?
2)in your h2o/h2o2 mix, is it 1:1 and how much to you recommend spraying?
3)how do you recommend breaking the inoculate up?
4)how do you know when the bags are completely colonized? is it better to leave them longer?
5)would it be harmful to open the bags before the fruiting is done?

last, i'd like to know what you think the downsides to this method are. higher risk of contamination? lower yield? thanks for your help.


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: kdub]
    #6484807 - 01/22/07 06:54 AM (17 years, 10 days ago)

COCKS
RISE FROM THE DEAD YOU FUCKING THREAD
<3 gretchen

1) Your water content should be just fine for the first flush if your pasteurized substrate was properly hydrated.
2) Uhm, splash of that, splish of this. Normal ratio I think is like 1:10 of 3% h2o2. But I just use a little bit of h2o2, Ive never measured it out. Its not super important that you use h2o2 water, just an added precaution.
3) If you re-read the tek, you will see the spawn is broken up in the giant Oven Bags, just crush it up through the bags. Dont be an idiot and pole through the bag, just break it up through the bag.
4) The bags are completely colonized..... when they are 100% white. Yeah you could leave it longer, but if you mixed your spawn really well, the inside should be just as colonized as the part you can see.
5) Why would you want to? Its not gonna be harmful, only possibly let in contams, but other than that it would be fine. In one aspect it might be good to open them, to give a big burst of fresh air, but then you are going to let some moisture out. So I have never done it, and never had any problems with not doing it.

Downsides? I dunno, possibly lower yeilds? But in my opinion the ease factor is worth it to me. Higher contam risk? no, and if you do have contams, they should be isolated and contained in individual bags, rather than say, one contamd casing fucking up your whole fruiting chamber.

COCKS


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6486260 - 01/22/07 03:29 PM (17 years, 10 days ago)

Quote:

hydr0420 said:
RISE FROM THE DEAD YOU FUCKING THREAD






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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6554689 - 02/11/07 04:18 PM (16 years, 11 months ago)

:royalrainbow:


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Re: Simple Invitro Poo Bags [Re: LosAngelesGraff]
    #6562644 - 02/13/07 11:58 AM (16 years, 11 months ago)

Which would be better to use on this TEK?
BRF/Verm cakes or Grain Rye Berry substrate?


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Offlineitsdatpurp
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Re: Simple Invitro Poo Bags [Re: Schism420]
    #6562761 - 02/13/07 12:29 PM (16 years, 11 months ago)

wow i remember when i first came to the shroomery i wanted to do this tek. i never got around to it. after reading it all over again i feel like i have too much supplies i never needed to begin with :tongue:

im looking online for spawn bags now :rasta:


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Re: Simple Invitro Poo Bags [Re: itsdatpurp]
    #6563713 - 02/13/07 05:20 PM (16 years, 11 months ago)

Quote:

itsdatpurp said:
wow i remember when i first came to the shroomery i wanted to do this tek. i never got around to it. after reading it all over again i feel like i have too much supplies i never needed to begin with :tongue:

im looking online for spawn bags now :rasta:




Got anything interesting you want to trade for some,I still have a bunch from when I did this tek and I sure as hell am not going to be doing it again


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #6566154 - 02/14/07 10:06 AM (16 years, 11 months ago)

This is the easiest way to grow. i was gonna do some monotubs after i made abunch of bags, but never had time for it. I just checked on the bags every other night and left them in a box in the closet, and picked them. thats it. i used quarts of wbs and hpoo/straw/vermic. but if you order a grain bag or something, you wont ever even need a pc.
i got .5 to 1.5oz-edit:dry- from each of 4 flushes. i didnt add more water or give it enough fae or light. on the 3rd flush they were huge weighing 70g-edit:wet- each, but some hit the top of the bag and became mangled, but it doesnt matter. i did eq and have b+'s coming up now.


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Edited by oso (02/14/07 10:07 AM)


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Re: Simple Invitro Poo Bags [Re: oso]
    #6572295 - 02/15/07 08:12 PM (16 years, 11 months ago)

bump for a great tek.. anyone else use it?


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Re: Simple Invitro Poo Bags [Re: NeoQ]
    #6669481 - 03/14/07 04:24 PM (16 years, 10 months ago)

Quote:

NeoQ said:
great post bro. We should call this Hydr0420-tek:)

Where did u get that Texas syringe?

I am a newbie but I got to ask: To spawn the jars to use in your tek, we can use the PF-tek to let the mycelium grow in the jars and use that as spawn?




It has been dubbed the "Dro Tek"...yes pf cakes work great.


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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #6669516 - 03/14/07 04:33 PM (16 years, 10 months ago)

Looks good . I , myself , think it's a waste of perfectly good spawn bags:grin:

I'd rather have those bags filled with spawn . But , I'm not lazy , lol:thumbup:

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Re: Simple Invitro Poo Bags [Re: HippieChick]
    #6729360 - 03/30/07 07:16 PM (16 years, 9 months ago)

sweet shit:thumbup:


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Re: Simple Invitro Poo Bags [Re: flushme]
    #6729390 - 03/30/07 07:26 PM (16 years, 9 months ago)

anyone used tasmanian or koh samui in bags? also, has anyone kinda stretched the spawn and not used a 1:1 poo/spawn ratio?


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Re: Simple Invitro Poo Bags [Re: kdub]
    #6865607 - 05/02/07 11:36 PM (16 years, 8 months ago)




COCKS!


CAW CAW!!!


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #6865680 - 05/02/07 11:57 PM (16 years, 8 months ago)

LoutfuckingL


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Re: Simple Invitro Poo Bags [Re: Cryogenicz]
    #6895370 - 05/09/07 09:11 PM (16 years, 8 months ago)

hey cry0 u poo is superior with this tek ty ty ty
beh those teks hippie3 posted look like way to much work, i hate that shit.

"Simple Invtiro Poo Bags"

not, invent the wheel again poobag.


Edited by jeetered (05/21/07 11:38 AM)


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #6982372 - 05/29/07 02:41 PM (16 years, 8 months ago)

!!RESURECTED AGAIN!!

Got a question on this tek. This looks friggin
awesome!

I am thinking about doing rye spawned to straw/cpoo 70/30.
Will this work? And is the filter patch enuf for FAE?

And has anyone else had any luck with this maybe using
a similar substrate as I plan on using?


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6982502 - 05/29/07 03:09 PM (16 years, 8 months ago)

I read that Blutjager used Mexicube and didn't get desireable results.

Has anyone else tried this tek with Mexicube? If so, what were your results?

I got a friend who may be doing this tek with some Mexicubes, but if it's most likely a failure I'll stop him.


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Re: Simple Invitro Poo Bags [Re: DragonHero]
    #6991662 - 05/31/07 01:57 PM (16 years, 7 months ago)

Mexicube performs horribly invitro in my experience, some strains don't like it.

here are my results using mycopath's 50/50 pre pasteurized hpoo. This is F+ strain. I had monotubs of mexicube that would not pin at all, and 1 poo bag that would not pin at all. Shitty invitro strain, but preforms awesomely on cakes.

THANKS DR0!!! This is off 3 of your invitro poo bags.







and some lone fruits from one of ohmatics monotubs:






and in the bags:









Edited by jeetered (05/31/07 02:01 PM)


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OfflineCryogenicz
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Re: Simple Invitro Poo Bags [Re: jeetered]
    #7416877 - 09/16/07 03:05 PM (16 years, 4 months ago)

Everyone needs to see this method.. Great Tek

-Graham


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Re: Simple Invitro Poo Bags [Re: Cryogenicz]
    #7417187 - 09/16/07 04:55 PM (16 years, 4 months ago)

works for non psilocybe species also.


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: jeetered]
    #7417522 - 09/16/07 06:50 PM (16 years, 4 months ago)

jeetered, i've been meaning to ask you how much poo and spawn you use for each of those bags? like 2 quarts poo 1 quart spawn or what?

peace


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Re: Simple Invitro Poo Bags [Re: headieherbs] * 1
    #7418219 - 09/16/07 10:23 PM (16 years, 4 months ago)

exactly what hydr0420 does.


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #7421578 - 09/17/07 09:44 PM (16 years, 4 months ago)

These are the shit. I do these in coir/coffee and get
nice flushes. Poo is definitely better though.


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Invisibledumbfounded1600
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Re: Simple Invitro Poo Bags [Re: Mycodood]
    #7421970 - 09/17/07 11:24 PM (16 years, 4 months ago)

yeah but what is that with smaller mushrooms getting higher psilocybin contents then bigger mushrooms


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Re: Simple Invitro Poo Bags [Re: dumbfounded1600]
    #7421987 - 09/17/07 11:32 PM (16 years, 4 months ago)

psilocin as well as psilocybin levels are genetic, and vary greatly if using multispore inoculation. The "harvest before the veil tears" is probably the best bet for best potency, but i still think it's genetic, and doesn't really matter, they r great wether the veil is torn and fully open, or closed capped, either way they rock. Wet better then dry too... that "small shroomers r more magical" is completely opinion, in my opinion.


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #7423098 - 09/18/07 10:51 AM (16 years, 4 months ago)

Jeetered, are you saying that you can
grow pans in these?


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Re: Simple Invitro Poo Bags [Re: Mycodood]
    #7423474 - 09/18/07 12:22 PM (16 years, 4 months ago)



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Offlineigloo
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #7423643 - 09/18/07 12:56 PM (16 years, 4 months ago)

Nice


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Re: Simple Invitro Poo Bags [Re: igloo]
    #7717176 - 12/04/07 08:02 PM (16 years, 1 month ago)



I was thinking about a thread I read recently about the benefits of late casing. Since, as seems to be popular opinion, subsequent flushes on poo cakes are not great, I was wondering if after the initial flush (done invitro in bags of poo) one could dunk then case lightly to achieve a better pinset? It seems like so much good substrate is wasted after the first flush....


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Re: Simple Invitro Poo Bags [Re: SoY]
    #7717196 - 12/04/07 08:06 PM (16 years, 1 month ago)

Awesome tek man ill have to try this on my next grow


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #7750488 - 12/12/07 06:03 PM (16 years, 1 month ago)

I just think some of the majick escapes when the viel breaks...the viel holds the majick in-but if you aren't using them for spiritual purposes I would suggest letting the viel break...a good way to make sure you don't have a breakthru experience, explore the astral universe, or find God.


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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #7750724 - 12/12/07 06:42 PM (16 years, 1 month ago)

Thanks for digging this one up. By digging this thread up, all of the magick escaped.


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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #7750837 - 12/12/07 07:00 PM (16 years, 1 month ago)

Quote:

Gretchenmeister said:
I just think some of the majick escapes when the viel breaks...the viel holds the majick in-but if you aren't using them for spiritual purposes I would suggest letting the viel break...a good way to make sure you don't have a breakthru experience, explore the astral universe, or find God.




WTF!?!?!  :justdontknow:


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Re: Simple Invitro Poo Bags [Re: mad genius]
    #8108726 - 03/05/08 10:02 PM (15 years, 10 months ago)

bump


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Normlizer420]
    #8108737 - 03/05/08 10:05 PM (15 years, 10 months ago)

Why hello there thread, I missed you!

Hope this bump can help some peoples.

<3 norm


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Invisibledurban_poison
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #8108881 - 03/05/08 10:44 PM (15 years, 10 months ago)

koh samui and tazmanians work great,australians and south africans also work great. dont let anybody discourage you from this tek if you make your own bags. i would discourage dung and advise coco/straw/eartwormcastings/brf/flax/amaranth/lime. if you mix it right it will look like dung with lots of persian white seeds(the amaranth). this tek is the most time efficent and reliable method if you have the right equipment= flowhood, bags, sterilizer(your choice 25qt or 41qt). you must also spawn those with grain for quick colonization. LC's work only if you have a very low moisture content which can be very tricky. as far as grain spawn is concerned you still want a low moisture content. it takes a couple practice bags but you will get it right. once you know it will always be perfect. if your moisture content is to high after they have colonized the exterior before the ending shake you can open the bag and drain the mycelium sweat of and seal with only tape instead of the impulse sealer in front of your flowhood. the reason most people complain about this tek or dont use it is because the equipment costs alot. remember proper mushroom cult costs as much as proper herb cult. expensive start up. however if you have 2 25qt all americans you can make 40 bags in a day. dont get me wrong its not a days work. this is how it rolls.....
day 1 make substrate and load 40 bags up.
day 2 sterilize all 40 bags and impulse seal.
day 3 spawn 40 bags.
day 4 move them to your storage area. no need to worry about incubation temps the bags will keep themselves warm enough.
day 5-7 sit back and relax till the new week starts and do it again.
now for you fruiting area. ikea metal shelves are the shit. i would recomend for this style 3 layers of shelving which will stand to the height of 6' 3". with 3 shelvels long which i dont remember the length but its will fill your avaerage room. not a long room your average apt/condo room. each bottom part of the bottom shelves will need lights. 41" flouresnts are great for this 2 lights per layer, you will have 4 layers so 8 lights. i would use the lowest wattage bulbs because they work just the same as higher wattage ones. you can fit 8 bags in each section. considering you have 3 sections long and 4 sections tall that means 1 of these units holds 98 bags. with the scehdule i provided you will need 2 of them to work all month totaling to 196 bags. these shelves will be on cycle. you should yeild anywhere from 1-1 1/2oz per bag first flush. its well worth the second flush for 1/2-1oz. after that its better to move them outside or throw them away. only worry is disposing of this much trash. good luck and this is the first and last time i will post this.


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OfflineFungi_x
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Re: Simple Invitro Poo Bags [Re: durban_poison]
    #8110462 - 03/06/08 10:55 AM (15 years, 10 months ago)

That is the coolest thing I have read here in a long time :thumbup:

I WANT PICTURES OF A CUBE SETUP LIKE THE ONE YOU SPEAK OF :heart:

Thanks, FX


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Re: Simple Invitro Poo Bags [Re: Fungi_x]
    #8111314 - 03/06/08 02:41 PM (15 years, 10 months ago)

FUK U!



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Re: Simple Invitro Poo Bags [Re: jeetered]
    #8112929 - 03/06/08 07:45 PM (15 years, 10 months ago)

JEETERED! woooo


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #8118446 - 03/08/08 12:52 AM (15 years, 10 months ago)

I gotta say my favorite part of these pictures is the NES & SNES hooked up in the background!


Mushies look good too however... I can totally understand your lazyness to bother with casings, even though your yield would be much greater I think.


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Invisibledurban_poison
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Re: Simple Invitro Poo Bags [Re: Fungi_x]
    #8543336 - 06/20/08 03:20 AM (15 years, 7 months ago)

okay i said i wouldnt show this tek but fuck it....





this is from 2 bags. yeilds can vary. 1-3 oz first flush then slowly decreasing by about a 1/4-1/2 ounce each flush till about the 4th which is a waste of time unless you throw them out side and they seem to have the original yields start over.


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Invisibledurban_poison
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Re: Simple Invitro Poo Bags [Re: durban_poison]
    #8553238 - 06/23/08 05:27 AM (15 years, 7 months ago)

tomorrow i will post what 8 harvasted bags look like since im out of the business for personal and health reasons.


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Re: Simple Invitro Poo Bags [Re: durban_poison]
    #8555101 - 06/23/08 04:53 PM (15 years, 7 months ago)

i am having a rash on mine.


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Re: Simple Invitro Poo Bags [Re: jeetered]
    #8555220 - 06/23/08 05:23 PM (15 years, 7 months ago)

you didn't even find the oakridge strain, liar


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Re: Simple Invitro Poo Bags [Re: BIGSWANG]
    #8555745 - 06/23/08 07:30 PM (15 years, 7 months ago)

Does this tek work with straight straw?

If it does it may benifit other strains that were poor preformers on straight poo.


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Re: Simple Invitro Poo Bags [Re: jccc]
    #8556144 - 06/23/08 09:02 PM (15 years, 7 months ago)

Yes it would. Although there is not much nutrients in straw. I'd cut it with a lot more.


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Offlinegdizzle
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #8568382 - 06/26/08 07:29 PM (15 years, 7 months ago)

what type of light is used and how long do you leave it on?


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Re: Simple Invitro Poo Bags [Re: gdizzle]
    #8568449 - 06/26/08 07:43 PM (15 years, 7 months ago)

Indirect light is all they need (either a room light or sun). Light just gives direction...nothing fancy needs to be done.


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Re: Simple Invitro Poo Bags [Re: MarioTrip]
    #8569044 - 06/26/08 10:09 PM (15 years, 7 months ago)

Uh...Wrong...Light gives somewhat of a direction of growth but more so weigh on wind currents, you can do this test yourself. Putting a fan on one side of the terrarium and see where they go while light is above them. Also lighting stimulates primordia.


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Re: Simple Invitro Poo Bags [Re: dumbfounded1600]
    #8569285 - 06/26/08 11:06 PM (15 years, 7 months ago)

Quote:

dumbfounded1600 said:
Uh...Wrong...Light gives somewhat of a direction of growth but more so weigh on wind currents, you can do this test yourself. Putting a fan on one side of the terrarium and see where they go while light is above them. Also lighting stimulates primordia.




I kind o agree, and, spectrum of light is imprtant, incandescent warm spectrum doesn't give optimal lighting whatsoever, i've found cool spectrum CFL's to be ideal.


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Re: Simple Invitro Poo Bags [Re: MarioTrip]
    #8707649 - 07/31/08 01:59 PM (15 years, 5 months ago)

do the lights need to be on a certain schedule? like 12/12 or can they just be on 24/7 for fruiting to occur?


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Re: Simple Invitro Poo Bags [Re: dawghead99]
    #8708299 - 07/31/08 04:22 PM (15 years, 5 months ago)

I have had fruit occur with 24/7 lighting. I have also heard that some people experience bigger fruit with shorter periods of light, but have never tried it myself.


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Re: Simple Invitro Poo Bags [Re: Phraggle]
    #8708310 - 07/31/08 04:25 PM (15 years, 5 months ago)

I use a regular lightbulb, 12/12, generates extra heat at night.


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Re: Simple Invitro Poo Bags [Re: shevanel]
    #8728388 - 08/05/08 10:43 AM (15 years, 5 months ago)

I have another question for anyone that has tried this tek and have had success..


How tall do the bags stand with the 1lb of poo/spawn mixture in it?

im trying to get some shelves for them to be placed on to fruit but dunno how far apart the shelves need to be. I heard the shrooms don't grow as big if they don't have enough area so i think its important for them to have optimal growth area....

I could be completely wrong cuz im a nubby.... answer my question someone please? it would be much appreciated.


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Re: Simple Invitro Poo Bags [Re: Joops]
    #8815479 - 08/23/08 12:19 PM (15 years, 5 months ago)

great guide, im about to try this out.


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Re: Simple Invitro Poo Bags [Re: shroomiin]
    #8941654 - 09/16/08 10:33 PM (15 years, 4 months ago)

this sounds to be an incredible tek, and i will be sure to try it out once my current PF tek is done with.  So whats the final word on what strains like this tek the best?


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Re: Simple Invitro Poo Bags [Re: mynameissupersteve]
    #8941933 - 09/16/08 11:46 PM (15 years, 4 months ago)

Quote:

kohschooley said:
this sounds to be an incredible tek, and i will be sure to try it out once my current PF tek is done with.  So whats the final word on what strains like this tek the best?




I've found these strains/spp to work:

texas
oak ridge
F+
palenque
syzygy
amazonian
tazmanian
pan cope cyan
lizard king
PESH
penis envy
albino penis envy
pe6
uncut
stropharia
and
Kentucky J


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Re: Simple Invitro Poo Bags [Re: wikid187]
    #8942346 - 09/17/08 01:27 AM (15 years, 4 months ago)

Im sure all the cube strains will work on manure invitro.


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Re: Simple Invitro Poo Bags [Re: Krez]
    #8951630 - 09/18/08 08:44 PM (15 years, 4 months ago)

some are very co2 intolerant, but shit try them all i say!


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Re: Simple Invitro Poo Bags [Re: Joops]
    #8953664 - 09/19/08 06:59 AM (15 years, 4 months ago)

seems expensive to me...thats more than ive spent one everything so far  but if it works ull also have alot more sub than me, good for you...  cant w8 to c results


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Re: Simple Invitro Poo Bags [Re: DoidleTheDigger]
    #8974725 - 09/23/08 06:56 PM (15 years, 4 months ago)

wikid thanks for the info on the strains.  I cant wait to get my next grow going once this ones over.  maybe im looking too much ahead but haha oh well.


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Re: Simple Invitro Poo Bags [Re: wikid187]
    #8975085 - 09/23/08 07:58 PM (15 years, 4 months ago)

Quote:

wikid187 said:
Quote:

kohschooley said:
this sounds to be an incredible tek, and i will be sure to try it out once my current PF tek is done with.  So whats the final word on what strains like this tek the best?




I've found these strains/spp to work:

texas
oak ridge
F+
palenque
syzygy
amazonian
tazmanian
pan cope cyan
lizard king
PESH
penis envy
albino penis envy
pe6
uncut
stropharia
and
Kentucky J



pan cope cyan ? you fruit invitro with them?


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Re: Simple Invitro Poo Bags [Re: grod31]
    #9028201 - 10/04/08 12:11 PM (15 years, 3 months ago)

I've been wondering, with what other bulk substrates could you do this with?  I saw someone ask about doing it with straigh straw, but are there anyother substances you could used in place of the 50/50 straw Hpoo and what effects would this have on the whole grow?


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Re: Simple Invitro Poo Bags [Re: Hippie3]
    #9156536 - 10/29/08 09:19 PM (15 years, 3 months ago)

Quote:

Hippie3 said:
i just re-read the thread
and he wasn't explicit as to
how long his spawn run took,
just that he began fanning
at the start of week 2.
presumbly that means it's finished, like yours,
in about a week.
but i do agree that filter patches would be improvement.
but perhaps even larger patches than what one gets
on commercial pre-made filter bags.
see  maximized invitro bag tek








this dudes a megalomaniac


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Re: Simple Invitro Poo Bags [Re: mynameissupersteve]
    #10039330 - 03/25/09 02:40 PM (14 years, 10 months ago)

bump for a great thread. a friend tried this tek a few years ago and it worked great.  he'd like to know the answer about different possible mediums besides 50/50 poo straw.


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Re: Simple Invitro Poo Bags [Re: Joops]
    #10040247 - 03/25/09 04:46 PM (14 years, 10 months ago)

tried a variation of this tek. works well.


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Re: Simple Invitro Poo Bags [Re: scatmanrav]
    #10040297 - 03/25/09 04:52 PM (14 years, 10 months ago)

did you get 300-600 grams from one bag or from all of them?


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Re: Simple Invitro Poo Bags [Re: living legends]
    #10040509 - 03/25/09 05:28 PM (14 years, 10 months ago)

we must be revisiting the past for all these newbies this week. This is like the 4th thread Ive seen brought back from the dead. i remember when this thread started years ago. But none the less this is a good one to bring back, i had forgotten about it in my vacation. And yeah its easily possible to get up to 400 grams fresh from each bag. check out some of the bag grows thats around here, we got some master's at this type of growing.


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Re: Simple Invitro Poo Bags [Re: Doodle]
    #10040792 - 03/25/09 06:26 PM (14 years, 10 months ago)

Make sure you have a strain that is very resistant to having next to no FAE or this will not work well.The 1st way I ever fruited was in bags in a similar manner,I did it with 4 different strains,3 failed (Completely,I mean not even 1 single fruit)and the only one that did fruit was Texas.I have seen PE get good results in bags in other threads on here but have never tried them myself


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #10042038 - 03/25/09 09:34 PM (14 years, 10 months ago)

good point blutjager.  just for anyone's info my friend used golden teachers very successfully with the bag method.  he will be trying to the tried and true texas strain next.


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Re: Simple Invitro Poo Bags [Re: ackmess]
    #10042093 - 03/25/09 09:40 PM (14 years, 10 months ago)

Texas almost never disappointed me,seems to not only not need to be babied but actually tries to get ahead and beat the odds and even grow in inhospitable conditions.. Like this old grow of mine >> http://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=7021766&fpart=1&PHPSESSID= :hehehe:


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #10042103 - 03/25/09 09:41 PM (14 years, 10 months ago)

And that was an accident :whoah:


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #10042143 - 03/25/09 09:47 PM (14 years, 10 months ago)

thats pretty incredible blut!  simply amazing.  even more glad he chose texas now.  :smile:


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Re: Simple Invitro Poo Bags [Re: ackmess]
    #10042186 - 03/25/09 09:51 PM (14 years, 10 months ago)

Thanks,funny how that was fated for the garbage and yet I managed to pull 9 dry oz off of it along with a ton of LC I cloned of of that big one.. I was happy to say the least


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #10042260 - 03/25/09 10:01 PM (14 years, 10 months ago)

well, since I don't want to start a new thread and am feeling slightly lazy my friend wants to know where the best place to score some pasteurized 50/50 poo/straw mix from now that tennstudd is no longer a sponsor?  its been a couple of years since he has paid attention to all this stuff.  he score plenty of horse shit from around locally but he doesn't want to go through pasteurizing.  HMC is still way too expensive, I just can't get over $50 for 10 pounds of dirt...


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Re: Simple Invitro Poo Bags [Re: ackmess]
    #10042678 - 03/25/09 11:05 PM (14 years, 10 months ago)

I don't know if that is directed to me or not but if it is your asking the wrong guy,the only times I ever did use manure was when tennstudd was a vendor here and to tell you the truth I could have a dozen horses in my living room and would still use coir,For me manure did only SLIGHTLY better but not even close to near being worth the extra work involved if I ha to leach and pasteurize it and all that extra stuff myself,when I got it all I had to do was mix in my spawn and even that was messy compared to dropping a block of coir in a bucket and dumping a gallon of water on it


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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #10043057 - 03/26/09 12:00 AM (14 years, 10 months ago)

how would one use coir instead of hpoo?  I am familiar with coir as a growing medium for pot.  could one do the invitro bag method with coir instead of hpoo?  it is much easier to buy coir than hpoo online.  thanks for the help


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Re: Simple Invitro Poo Bags [Re: ackmess]
    #10044581 - 03/26/09 09:11 AM (14 years, 10 months ago)

Quote:

ackmess said:
bump for a great thread. a friend tried this tek a few years ago and it worked great.  he'd like to know the answer about different possible mediums besides 50/50 poo straw.




You can do 100% hpoo, also hpoo and verm, coir and hpoo.  There are several kits on the market that advertise invitro grain bag kits (have not heard  many reports of success with any type of "grow kit")...and I know a few people who have tried doing wbs and rye invitro bag style.  My conclusion is that grains just dont hold the moisture needed to sustain a self supporting environment, poo/straw does...plus the microbial benefits of using poo or a poo mix is an added attraction of this substrate-invitro bag teks do not get as much FAE as most other teks...FAE is a big factor in keeping contams down...properly prepared poo withstands this environment with minimized chances for contams with this tek...as opposed to other spawn mediums.


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Offlinescifipirate
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #10045302 - 03/26/09 11:45 AM (14 years, 10 months ago)

Fuck yeah dude :bow2:


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OfflineMycoMario
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Re: Simple Invitro Poo Bags [Re: ackmess]
    #10045331 - 03/26/09 11:50 AM (14 years, 10 months ago)

Quote:

ackmess said:
well, since I don't want to start a new thread and am feeling slightly lazy my friend wants to know where the best place to score some pasteurized 50/50 poo/straw mix from now that tennstudd is no longer a sponsor?  its been a couple of years since he has paid attention to all this stuff.  he score plenty of horse shit from around locally but he doesn't want to go through pasteurizing.  HMC is still way too expensive, I just can't get over $50 for 10 pounds of dirt...




I have tons of horse poop. Shoot me a PM.


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Offlinescifipirate
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Re: Simple Invitro Poo Bags [Re: scifipirate]
    #10045359 - 03/26/09 11:55 AM (14 years, 10 months ago)

Quote:

scifipirate said:
Fuck yeah dude :bow2:





hah, just realized how old this thread is


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Re: Simple Invitro Poo Bags [Re: Gretchenmeister] * 1
    #10927793 - 08/24/09 04:04 PM (14 years, 5 months ago)

just wanted to bump this thread for any newbies looking to make a jump to poo or compost


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Offlineremykwp
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Re: Simple Invitro Poo Bags [Re: Gretchenmeister]
    #14142486 - 03/18/11 10:29 AM (12 years, 10 months ago)

Could this be done with coir?


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Offlineremykwp
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Re: Simple Invitro Poo Bags [Re: remykwp]
    #14147244 - 03/19/11 06:09 AM (12 years, 10 months ago)

bump


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Offlineremykwp
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Re: Simple Invitro Poo Bags [Re: remykwp]
    #14182768 - 03/25/11 05:23 PM (12 years, 10 months ago)

bump on coir question.


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Offlineremykwp
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Re: Simple Invitro Poo Bags [Re: remykwp]
    #15151625 - 09/29/11 08:23 AM (12 years, 4 months ago)

I am just confirming that this can be done with coir.
I dont see why hydro says 2nd and 3rd flushes are junk.
I have found it only gets better with each flush. Ranging a quarter pound dry for 1 bag, 1st flush = 1oz dry, 2nd flush = 1.5oz dry (monsters on this flush), and 2nd and 3rd together ranged about 1.5.

I use 1 qt jar of spawn and 2 quart jars loosely packed per bag.


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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #16269857 - 05/22/12 08:01 PM (11 years, 8 months ago)

AH-HA!

IT DOES WORK

GOD BLESS THIS PERSON


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Offlinehunterthompson5
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Re: Simple Invitro Poo Bags [Re: remykwp]
    #16435466 - 06/25/12 11:45 AM (11 years, 7 months ago)

Quote:

remykwp said:
I am just confirming that this can be done with coir.
I dont see why hydro says 2nd and 3rd flushes are junk.
I have found it only gets better with each flush. Ranging a quarter pound dry for 1 bag, 1st flush = 1oz dry, 2nd flush = 1.5oz dry (monsters on this flush), and 2nd and 3rd together ranged about 1.5.

I use 1 qt jar of spawn and 2 quart jars loosely packed per bag.




I feel like a shaman reviving this thread :crazy2:

I have some colonizing jars and was thinking of using this tek. Had a couple qs though:
1. So coir can be used instead of hpoo/straw?  Do you just substitue it in the same proportions and everything?
2. Any good links for hpoo/straw? How about this http://www.out-grow.com/index.php?main_page=product_info&cPath=4&products_id=68


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Offlineremykwp
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Re: Simple Invitro Poo Bags [Re: hunterthompson5]
    #16478868 - 07/04/12 04:48 AM (11 years, 6 months ago)

Quote:

1. So coir can be used instead of hpoo/straw?  Do you just substitue it in the same proportions and everything?
2. Any good links for hpoo/straw? How about this http://www.out-grow.com/index.php?main_page=product_info&cPath=4&products_id=68




1. Yeah, your spawn ratio is your choice, I preffer 1 part spawn to 2 or 3 parts bulk (I have an old house and cannot be carefull enough)

2. I have mainly used coir due to RogerRabbit's persistence on the subject. Horse shit can be easily had, just drive around the countryside until you see a few stallions, and go bother their owners.

I might be able to help you out with some poo but you would have to pay shipping. PM me so we don't break rules(This is the wrong forum to be posting this stuff). I'm pretty sure a priority mail box would handle a good amount of shit.


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Re: Simple Invitro Poo Bags [Re: remykwp]
    #16479542 - 07/04/12 09:47 AM (11 years, 6 months ago)

Quote:

remykwp said:
Quote:

1. So coir can be used instead of hpoo/straw?  Do you just substitue it in the same proportions and everything?
2. Any good links for hpoo/straw? How about this http://www.out-grow.com/index.php?main_page=product_info&cPath=4&products_id=68




1. Yeah, your spawn ratio is your choice, I preffer 1 part spawn to 2 or 3 parts bulk (I have an old house and cannot be carefull enough)

2. I have mainly used coir due to RogerRabbit's persistence on the subject. Horse shit can be easily had, just drive around the countryside until you see a few stallions, and go bother their owners.

I might be able to help you out with some poo but you would have to pay shipping. PM me so we don't break rules(This is the wrong forum to be posting this stuff). I'm pretty sure a priority mail box would handle a good amount of shit.




Thanks a bunch! I'm atually all set now but thanks so much for the offer. I may take you up on that on my next grow :smile:


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OfflineDr.Mind_BendEr
Mad Scientist


Registered: 04/11/13
Posts: 39
Loc: The Farm
Last seen: 10 years, 7 months
Re: Simple Invitro Poo Bags [Re: hydr0420]
    #18208380 - 05/03/13 01:46 PM (10 years, 8 months ago)

Bump! :thisisheavyman:


--------------------


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OfflinePhoenix117
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Registered: 11/29/13
Posts: 2
Last seen: 10 years, 1 month
Re: Simple Invitro Poo Bags [Re: Dr.Mind_BendEr]
    #19222211 - 12/03/13 01:17 PM (10 years, 1 month ago)

How would invitro work with maztepec, kph samui, and mestizo? The spores are enroute and I'm not sure whether I should use bulk sub and case or use this. I'm diving in head first with wbs. As this will be my first grow


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OfflinePhoenix117
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Registered: 11/29/13
Posts: 2
Last seen: 10 years, 1 month
Re: Simple Invitro Poo Bags [Re: Phoenix117]
    #19222314 - 12/03/13 01:43 PM (10 years, 1 month ago)

Koh samui** my bad


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Offlinerigadoog
Stranger
Registered: 05/31/13
Posts: 41
Last seen: 9 years, 1 month
Re: Simple Invitro Poo Bags [Re: Phoenix117]
    #19231777 - 12/05/13 03:29 PM (10 years, 1 month ago)

I want to do this tek but im only going to have 4 bags with the equivalent of about a half-pint jar of substrate. This would come out to using 2 lbs of poo using the given ratio, but do you guys think I could stretch my initial mycelia and try to colonize, say, 5 lbs?


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Offlinerigadoog
Stranger
Registered: 05/31/13
Posts: 41
Last seen: 9 years, 1 month
Re: Simple Invitro Poo Bags [Re: rigadoog]
    #19235288 - 12/06/13 06:10 AM (10 years, 1 month ago)

Also, would it be wise to put the poo/straw in the oven or something in order to try and kill any contams it might have? Or do you think they do that before they ship it?


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