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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Outer]
    #6105398 - 09/26/06 10:04 PM (17 years, 4 months ago)

Damn kids. I havent been on the boards much in the past couple months, im suprised to see this thread still kicking around. I may just have to pay more attention to this shit, to help answer questions for people trying this.

Scatmanrav; Im flattered you are trying this method, I was always impressed with your other grows. PM me, I would like to hear your experiences with it.

Im glad bags are catching on.


Quote:

Outer said:
Im thinking this might be the best bet for me also since i need to be a little more on the stealthy side!I was wondering how sterile do you need to be doing this?What i mean is did you use a flowhood(dont see one but jsut asking? Or just a good whipe down with alcohol , some oust, gloves ,face mask etc. when you were mixing the poo and spawn jars!

cheers,
Outer




Honestly the only time you need to be sterile is when you make your spawn jars. Once you are working with pasteurized poo, you dont have to worry as much.
I wiped down the bathroom with bleach, ran a cheapass walmart HEPA filter in there for an hour, then did everything OPEN AIR. Half the time I didnt even wash my hands. You may not have as good of results. But when you are working with pasteurized substrates its not sterile, so you dont have to be, just be clean and careful.


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Offlinebw86
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6275548 - 11/12/06 02:04 PM (17 years, 2 months ago)

I have a couple questions.

After letting them colonize in the dark I need to place them in a closet with light? Should I let the light beat down directly on the bags or should they be out of direct light? Do you leave the closet door open or closed. Also it says that the lights are on timers - what are good settings to use? Would intense blue leds help at growth at all?


Edited by bw86 (11/12/06 02:39 PM)


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: bw86]
    #6275850 - 11/12/06 03:47 PM (17 years, 2 months ago)

I have Q's too, it looks like you have the bags in a martha to fruit.. I briefly glanced at the tek but I thought you said they kept humidity in very well... Could you just fruit them on a shelf in open air somewhere or would they need to be in a humid environment... Also, is the sealer part of a vacuum sealer (foodsaver) an impulse sealer? I obviously wouldn't use the vaccum part of it just the seal feature.... an where can I get those bags??



NICE FUCKING WRITE UP tho! If you can answer those questions for me I'll be trying it for sure! I like your style...


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InvisibleBIGSWANG
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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6275864 - 11/12/06 03:51 PM (17 years, 2 months ago)

get a 12 inch impulse sealer off of ebay headie...unless you already have a food saver with a sealer...

and you can leave the bags on a shelf....there is no need for external humidity

and you can get the bags from www.sporeworks.com


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: BIGSWANG]
    #6275914 - 11/12/06 04:01 PM (17 years, 2 months ago)

thanks dawg why doesn't everyone grow this way...!!?!?!

I know why, I'm just a not a perfectionist like most everyone else...

Thanks BIGSWANG AND DRO!


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InvisibleBlutjager
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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6276201 - 11/12/06 05:28 PM (17 years, 2 months ago)

I did this exact same thing a while back,out of 17 bags 4 of them fruited and even that was after waiting so long that I cut the tops off and started fanning,because nothing useful will get through the filter patch,O and it wasn't like the other ons contaminated or anything,they just stalled and took so long to pin that they gave up and didn't even try,I thought this would have been a nice painless way to grow,and it would be if I had better results,It is a good tek but you will have to be very patient and wait forever to see anything,the ones that did fruit were colonized for a month before I saw pins


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Offlinehightimesreader
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Re: Simple Invitro Poo Bags [Re: rxKaffee]
    #6276338 - 11/12/06 06:00 PM (17 years, 2 months ago)

Quote:

rxKaffee said:
HELL YES! thnx thnx thnx.

hom0420 : you are the man!




Hey rxKaffee, its Hydr0420 not hom0420....


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I'm hunting for The Following ethnos.
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A new leaf turned over.. I'm too old for this shit.
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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: headieherbs]
    #6276875 - 11/12/06 08:37 PM (17 years, 2 months ago)

Quote:

headieherbs said:
I have Q's too, it looks like you have the bags in a martha to fruit.. I briefly glanced at the tek but I thought you said they kept humidity in very well... Could you just fruit them on a shelf in open air somewhere or would they need to be in a humid environment... Also, is the sealer part of a vacuum sealer (foodsaver) an impulse sealer? I obviously wouldn't use the vaccum part of it just the seal feature.... an where can I get those bags??



NICE FUCKING WRITE UP tho! If you can answer those questions for me I'll be trying it for sure! I like your style...




Wow, this thread is still alive.

I know this has already been answered, but.
The shelves the bags are on are not in a martha, they are cheap plastic shelves from target. Its like 25$ for a 4 shelf setup that can fit 48bags.
The sealer is just a normal Impulse Sealer which can be had from eBay or search google for impulse sealer. Theres a company called Cleveland something or other, which has some of the best impulse sealer prices.

Quote:

Blutjager said:
I did this exact same thing a while back,out of 17 bags 4 of them fruited and even that was after waiting so long that I cut the tops off and started fanning,because nothing useful will get through the filter patch,O and it wasn't like the other ons contaminated or anything,they just stalled and took so long to pin that they gave up and didn't even try,I thought this would have been a nice painless way to grow,and it would be if I had better results,It is a good tek but you will have to be very patient and wait forever to see anything,the ones that did fruit were colonized for a month before I saw pins




This isnt always true. Some strains/substrains just do better in the bag conditions. I have tried strains like EQ that did shit, but every other strain did fine for me. Your results may vary, just like with any other method.

I have done sets of 80+ bags in the past with the exact same cultured spawn jars, all made at the same time in the same conditions, and 2 got green, and 4 never fruited. Its fucking random and weird, there is no reason for that, since the bags were made in 8bag batches from the same spawn jars. Shouldnt all 8 made from the same jar/poo mixed at the same time contamd? or stalled?

Mushrooms when done from multispore are gonna be random. Myc is gonna be random sometimes.

This will work for some people, not for all.


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InvisibleBlutjager
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6276985 - 11/12/06 09:09 PM (17 years, 2 months ago)

Yea,I do think it has something to do with strain,The only ones that did fruit at all were Texas(Of coarse,they even fruit in jars if ya leave them alone)Even some of them didn't work however,The treasure coast did miserable and so did the mexicub and Ecuador.O well I guess you just gotta take your chances if you want to set it and forget it


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: Blutjager]
    #6277008 - 11/12/06 09:13 PM (17 years, 2 months ago)

Quote:

Blutjager said:
Yea,I do think it has something to do with strain,The only ones that did fruit at all were Texas(Of coarse,they even fruit in jars if ya leave them alone)Even some of them didn't work however,The treasure coast did miserable and so did the mexicub and Ecuador.O well I guess you just gotta take your chances if you want to set it and forget it




Meh,
You should have got more yeild, everyone else I have talked to who has tried it has had decent results. What were the closet conditions?


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Offlineautographguys
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6277602 - 11/12/06 11:58 PM (17 years, 2 months ago)

I think a tub on tub would produce way more. Bags wont get enough fae


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: autographguys]
    #6277624 - 11/13/06 12:05 AM (17 years, 2 months ago)

dro.... I have several isolation prints of the hawaiian strain... how have they performed for you with this setup... I'm strongly considering trying this method before I get into a more control oriented grow, cause of lack of funds..... I love a lazyman's view, cause I'm a LAZY MAN.... input appreciated!


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: autographguys]
    #6280623 - 11/13/06 09:52 PM (17 years, 2 months ago)

Quote:

autographguys said:
I think a tub on tub would produce way more. Bags wont get enough fae




Yeah, as you can tell from the pics in the first post this method sucks, and there isnt much FAE. I think you should go post in threads about tubs instead if you dont like my method, its not for you.
You should also probably e-mail mycobag to let them know to stop selling their bags that they say you can fruit directly in the bag. Since it doesnt work.

Quote:

headieherbs said:
dro.... I have several isolation prints of the hawaiian strain... how have they performed for you with this setup... I'm strongly considering trying this method before I get into a more control oriented grow, cause of lack of funds..... I love a lazyman's view, cause I'm a LAZY MAN.... input appreciated!




I have used a HAW syringe before, it worked very well. Yours will be different from mine, but it should work. Like I said before, not every strain works in bags, just try it out and see if you like it or not.


Edited by hydr0420 (11/13/06 09:55 PM)


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Invisibleoso
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6280694 - 11/13/06 10:18 PM (17 years, 2 months ago)

how bad did eq do? cause i was gonna unload my jars soon, would i be better off fruiting outside the bag on those? and i also have b+ and pink buf jars getting ready, which is the best for leaving them in the bags? i think im gonna move this soon. nice tek though hydro.


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OfflineMisterMudd
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Re: Simple Invitro Poo Bags [Re: oso]
    #6280718 - 11/13/06 10:27 PM (17 years, 2 months ago)

Can you remove the bricks from the bags and place them into a FC terrarium and fruit them like cakes? The only reason I think I would do that is to allow more room for the little guys to stretch out.


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Invisibleoso
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Re: Simple Invitro Poo Bags [Re: MisterMudd]
    #6280734 - 11/13/06 10:33 PM (17 years, 2 months ago)

yea, i was thinking about that for the eq after reading it didnt do well in bags


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"When tyranny and oppression come to this land, it will be in the guise of fighting a foreign foe."
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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: MisterMudd]
    #6280756 - 11/13/06 10:38 PM (17 years, 2 months ago)

Quote:

MisterMudd said:
Can you remove the bricks from the bags and place them into a FC terrarium and fruit them like cakes? The only reason I think I would do that is to allow more room for the little guys to stretch out.




Sure, but that kinda defeats the whole purpose of doing bags invitro. You might as well just make normal casings or tubs with the same poo. The whole reason I did the bags is because once you make them, you dont have to do anything but occasionally monitor temps, and flip a lightswitch for a couple weeks, then pick.
Them stretching out doesnt matter in my experience, they keep growing even when they hit the top of the bag, usually curl back down.


Both of those were grown in bags.

Quote:

oso said:
how bad did eq do? cause i was gonna unload my jars soon, would i be better off fruiting outside the bag on those? and i also have b+ and pink buf jars getting ready, which is the best for leaving them in the bags? i think im gonna move this soon. nice tek though hydro.




EQ did total shit for me, but I am not saying it wont always work. Your multispore inoc may do better than mine, I only tried it once in bags with I think 10 bags, but in all honesty, the eq never did well for me in casings in a martha, or in casings in a PMP, my syringe may have just been shitty.
Give it a shot, theres only one way to tell if its going to work for you.


Edited by hydr0420 (11/13/06 10:39 PM)


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Offlineheadieherbs
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6323490 - 12/01/06 07:21 AM (17 years, 2 months ago)

hey hydro... sorry to reawaken this thread but would straight poo work as well as the 50-50 poo/straw mix? I ordered some pre-pasteurized from tennstud but it's straight poo.... someone please let me know quick I just ordered it a few days ago so I could probably change my order..... on another note, Ihaven't heard anythign from mike, how long does it take the shit to come?


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Offlinekdub
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Re: Simple Invitro Poo Bags [Re: hydr0420]
    #6481499 - 01/21/07 01:04 AM (17 years, 12 days ago)

i was recommended this method by gretchen and i am interested in using it as my first attempt at growing. a few q's though:

1)it is not necessary to add water to the bags for the first flush?
2)in your h2o/h2o2 mix, is it 1:1 and how much to you recommend spraying?
3)how do you recommend breaking the inoculate up?
4)how do you know when the bags are completely colonized? is it better to leave them longer?
5)would it be harmful to open the bags before the fruiting is done?

last, i'd like to know what you think the downsides to this method are. higher risk of contamination? lower yield? thanks for your help.


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Offlinehydr0420
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Re: Simple Invitro Poo Bags [Re: kdub]
    #6484807 - 01/22/07 06:54 AM (17 years, 11 days ago)

COCKS
RISE FROM THE DEAD YOU FUCKING THREAD
<3 gretchen

1) Your water content should be just fine for the first flush if your pasteurized substrate was properly hydrated.
2) Uhm, splash of that, splish of this. Normal ratio I think is like 1:10 of 3% h2o2. But I just use a little bit of h2o2, Ive never measured it out. Its not super important that you use h2o2 water, just an added precaution.
3) If you re-read the tek, you will see the spawn is broken up in the giant Oven Bags, just crush it up through the bags. Dont be an idiot and pole through the bag, just break it up through the bag.
4) The bags are completely colonized..... when they are 100% white. Yeah you could leave it longer, but if you mixed your spawn really well, the inside should be just as colonized as the part you can see.
5) Why would you want to? Its not gonna be harmful, only possibly let in contams, but other than that it would be fine. In one aspect it might be good to open them, to give a big burst of fresh air, but then you are going to let some moisture out. So I have never done it, and never had any problems with not doing it.

Downsides? I dunno, possibly lower yeilds? But in my opinion the ease factor is worth it to me. Higher contam risk? no, and if you do have contams, they should be isolated and contained in individual bags, rather than say, one contamd casing fucking up your whole fruiting chamber.

COCKS


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