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Offlinenambi
journeyman
 User Gallery
Registered: 10/18/02
Posts: 132
Last seen: 14 years, 27 days
Grain Transfers
    #1402032 - 03/23/03 03:15 AM (21 years, 4 months ago)

I have a B+ strain that is cultivating about 6 Jars on rye nicely, though I would like to have a few more jars though my Print is all used up. I was thinking about transferring cultivated grain to a set of new jars, though I am confused about contamination, since my preparation for the jars were very sterile.

If I were to open up the jars after they had been pressure cooked to introduce cultivated grains wouldn't a lot of contaminants get inside the jar during this process? How can I prevent this from happening if I were to do some grain transfers?

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Grain Transfers [Re: nambi]
    #1402045 - 03/23/03 03:26 AM (21 years, 4 months ago)

The key is to be clean and fast. Get everything ready beforehand, spray the entire area with Lysol and do your tranfers QUICKLY.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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OfflineAudi0
have ur cake andbeat it 2
Female User Gallery

Registered: 06/27/02
Posts: 10,334
Loc: Dirty South !
Last seen: 13 years, 18 days
Re: Grain Transfers [Re: dog]
    #1402065 - 03/23/03 03:37 AM (21 years, 4 months ago)

i turn the broiler on in my oven and let it warm up 180F, then open the door and set the jars in. have a spoon presterilized and oven mitts are a good idea too. if you work inside the oven with the doors open and it pretty hot you can have good success, just work fast bc its hot and also the quicker you do it the less chance for contam to get in.


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InvisibleArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
Re: Grain Transfers [Re: nambi]
    #1402076 - 03/23/03 03:42 AM (21 years, 4 months ago)

*Deleted*


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Edited by ArmFromTheAbyss1 (03/23/03 03:45 AM)

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Invisibledeanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
Re: Grain Transfers [Re: nambi]
    #1402184 - 03/23/03 04:38 AM (21 years, 4 months ago)


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InvisibleShroomismM
Space Travellin
Male User Gallery
Folding@home Statistics
Registered: 02/13/00
Posts: 66,015
Loc: 9th Dimension
Trusted Cultivator
Re: Grain Transfers [Re: nambi]
    #1402224 - 03/23/03 04:52 AM (21 years, 4 months ago)

The oven tek is great and works amazingly well for me. I have an extremely low contam rate doing G2G transfers using the oven.


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Invisiblegeorge castanzaM
Lord Of The Idiots!
Male User Gallery
Registered: 10/21/02
Posts: 8,762
Trusted Cultivator
Re: Grain Transfers [Re: Shroomism]
    #1402806 - 03/23/03 08:42 AM (21 years, 4 months ago)

if you can afford one a hepa filter is the only way to go
say kramer anyways


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KRAMER CAKES



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: Grain Transfers [Re: nambi]
    #1403896 - 03/23/03 03:49 PM (21 years, 4 months ago)

add 5ml h2o2per pint of substrate, or more if you're feeling paranoid, to the sterilised jars, and shake the fuck out of them before adding colonised material. The h2o2 kills off any stray spores that might float into the jars while you're transferring, and the colonised grains should resist contams anyway.


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hmmm........

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