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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Rye Grain, Wild Bird Seed

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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
grain to grain transfer?
    #1313618 - 02/17/03 10:08 AM (13 years, 9 months ago)

how much substrate would one use, would a table spoon of colonized grain be enough for a quart?


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Offlinesirreal
devoid
Registered: 01/11/03
Posts: 1,775
Loc: In the borderlands
Last seen: 9 years, 7 months
Re: grain to grain transfer? [Re: StainItBlue]
    #1313626 - 02/17/03 10:13 AM (13 years, 9 months ago)

Someone I know used about one tablespoon to inoculate 6 pint birdseed jars and they were fully colonised in less than a week! Just shake the jars up real good and get the moisture content right. good luck.


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I may not always tell the truth, but atleast I'm honest
-----------

I see what everyone is saying. It is so hard to form an opinion when you see both sides so clearly!


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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
Re: grain to grain transfer? [Re: sirreal]
    #1313639 - 02/17/03 10:28 AM (13 years, 9 months ago)

how soon after you inoculate it do you see growth, my buddy did it 2 days ago and sees no new growth, and its only supposed to take a week?


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Offlinesirreal
devoid
Registered: 01/11/03
Posts: 1,775
Loc: In the borderlands
Last seen: 9 years, 7 months
Re: grain to grain transfer? [Re: StainItBlue]
    #1313691 - 02/17/03 10:48 AM (13 years, 9 months ago)

Well, I do not know how your friend prepared the jars. Nor do I know the conditions they are kept in. There might be a reason that your jars are going more slowly. After 2 days this other persons jars had grown noticably. If you care to share maybe you can be helped.

Temp. could be a reason. How warm does he keep the jars? Maybe it was exposed to a contam during the transfer?


--------------------
I may not always tell the truth, but atleast I'm honest
-----------

I see what everyone is saying. It is so hard to form an opinion when you see both sides so clearly!


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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
Re: grain to grain transfer? [Re: sirreal]
    #1313711 - 02/17/03 10:56 AM (13 years, 9 months ago)

205 ml water ,200 ml rye grain..pc for an hour..incubating 83 degrees...the multi spore jars he does takes about 12 days to 100% colinize but for some reason these aint doin shit..he said hes just gonna leave em colonize hes in no rush thx for the responses


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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
Re: grain to grain transfer? [Re: StainItBlue]
    #1313713 - 02/17/03 10:57 AM (13 years, 9 months ago)

the temp is fine and no contams..just gonna see what happens..


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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
Re: grain to grain transfer? [Re: StainItBlue]
    #1313732 - 02/17/03 11:04 AM (13 years, 9 months ago)

well i just found out this kid inoculated them on the 16th,,so its only been a day..goddamn kid is so annoying..


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Offlinesirreal
devoid
Registered: 01/11/03
Posts: 1,775
Loc: In the borderlands
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Re: grain to grain transfer? [Re: StainItBlue]
    #1313737 - 02/17/03 11:07 AM (13 years, 9 months ago)

My friend used birdseed and found it easy to get the moisture content right. He has never worked with rye,so , good luck.


--------------------
I may not always tell the truth, but atleast I'm honest
-----------

I see what everyone is saying. It is so hard to form an opinion when you see both sides so clearly!


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Offlinesirreal
devoid
Registered: 01/11/03
Posts: 1,775
Loc: In the borderlands
Last seen: 9 years, 7 months
Re: grain to grain transfer? [Re: StainItBlue]
    #1313741 - 02/17/03 11:08 AM (13 years, 9 months ago)

:blush:............. :grin:


--------------------
I may not always tell the truth, but atleast I'm honest
-----------

I see what everyone is saying. It is so hard to form an opinion when you see both sides so clearly!


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: grain to grain transfer? [Re: StainItBlue]
    #1313832 - 02/17/03 11:46 AM (13 years, 9 months ago)

the TMC recommends 1-10 ratio for G2G transfer .but the original or master jar was started with only a couple of small agar wedges, so 1 Tbl. spoon would work, just not as fast .
i've seem in the gro-log forum others using as much as 1-5 ratios .
more = faster = less chance for contamination to get it's big foot in the door . :smile:
hope that helps your friend . :smirk:   


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OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: grain to grain transfer? [Re: deanofmean]
    #1313890 - 02/17/03 12:10 PM (13 years, 9 months ago)

I've done two successful grain to grain transfers...both with different types of mycelium.  I always use a pint of colonised rye to 4 pints uncolonised.  But this IS overkill!!  My jars all finished in four days though....but i could easily do 8 with a pint and they'd be done within a week for sure!  I find grain to grain is the way to go because colonisation is done sooooo much faster. :grin:


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Rye Grain, Wild Bird Seed

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