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toreachnirvana
newbie
Registered: 03/06/03
Posts: 61
Last seen: 18 years, 2 months
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grain to grain transfers ?
#1497558 - 04/27/03 09:31 AM (20 years, 9 months ago) |
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how many " generations " can you go when transfering myc from rye to rye. can one say turn 8 jars rye to 128 or beyond ?
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dog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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In TMC, the author demonstrates turning 1 grain jar into 100 using g2g transfers, so 8 jars should give you hmmm, lets see, about 800.
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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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toreachnirvana
newbie
Registered: 03/06/03
Posts: 61
Last seen: 18 years, 2 months
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Re: grain to grain transfers ? [Re: dog]
#1497637 - 04/27/03 10:13 AM (20 years, 9 months ago) |
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thanks so there is no degeneration to the myc then ? that sounds good to me
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Mike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 2 years, 1 month
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Re: grain to grain transfers ? [Re: dog]
#1497645 - 04/27/03 10:18 AM (20 years, 9 months ago) |
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Dog
I was searching also for a documented recommended limit for generations produced by myc transfer or cloning, until it would be advisable to break that up with a generation of multispore innoc......do you know ?
-------------------- your inside is out, and your outside is in.
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dog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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Quote:
thanks so there is no degeneration to the myc then ? that sounds good to me
There is degradation after the third generation according to TMC. In his demonstration he used a petri to inoculate 10 jars, these 10 jars to inoculate 100 more via g2g, then those 100 to do 1000.
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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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Mike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 2 years, 1 month
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Re: grain to grain transfers ? [Re: dog]
#1499074 - 04/27/03 09:46 PM (20 years, 9 months ago) |
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Do you know how the degradation expresses itself?
I'm certainly willing to believe Stamets regarding the three generations..... but I wonder what characteristics the mycelium.....or fruits....show to demonstrate that there is a negative direction?
-------------------- your inside is out, and your outside is in.
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SixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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My foaf goes out 4 generations, with no discernable ill effect.
From G1, he marks, dates & holds out & refridgerates several jars, to stall growth.
Same for G2 & G3.
At G4, while cropping out, he goes back to the G1 stored jars & G2G's them into more jars. So on & so forth, with each generation, until about 1 year has past & the G trail is about to end.
He then goes back to a G1 print & repeats the process.
In other woirds - IMHO, with thought, you can go out about one year from day 1 of the 1st muli/spore innoc if running crop after crop.
LOL, if you have multiple strains, you need a stand alone -- dedicated fridge (or 2), for jar culture storage. Hint. Seal jars tightly, otherwise they will dehydrate.
6T
-------------------- ~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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Mike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 2 years, 1 month
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Re: grain to grain transfers ? [Re: SixTango]
#1499190 - 04/27/03 10:22 PM (20 years, 9 months ago) |
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6T, please also see my post above.....how does the degradation show itself?
-------------------- your inside is out, and your outside is in.
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SixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: grain to grain transfers ? [Re: Mike Elium]
#1499244 - 04/27/03 10:39 PM (20 years, 9 months ago) |
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Quote:
6T, please also see my post above.....how does the degradation show itself?
What my foaf has seen was that, In a constant controled enviroment, it will demo itself as less rizo, slower substrate colonization, dwarfs, mutants & far less yeild.
6T
-------------------- ~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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NewSpore
Just Starting toSee the Light

Registered: 03/26/03
Posts: 467
Last seen: 14 years, 4 months
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Re: grain to grain transfers ? [Re: SixTango]
#1538787 - 05/11/03 09:43 AM (20 years, 8 months ago) |
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Hey Six, what would you consider slow substrate colonization? And if mustants are present should a friend assume that he has spores from generations past the "Proper Limit" ............... If that were true how does anyone really know what they are getting?
-------------------- Be in truth and watch the magic happen. SBP TEK
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micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: grain to grain transfers ? [Re: NewSpore]
#1538806 - 05/11/03 10:05 AM (20 years, 8 months ago) |
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Spores don't count. They are pretty random, though, so who knows what you're getting? Multispore culture is ok, IMO, because a certain dominant strain will take over.
There can be substrains with some wierd idiosincrasies, though. This is why cloning helps.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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