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Amazon Shop: ½ Pint Jars, Dehydrator, Paul Stamets, Petri Dish, Rye Grain

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Offlinetoreachnirvana
newbie
Registered: 03/06/03
Posts: 61
Last seen: 12 years, 6 months
grain to grain transfers ?
    #1497558 - 04/27/03 11:31 AM (15 years, 30 days ago)

how many " generations " can you go when transfering myc from rye to rye. can one say turn 8 jars rye to 128 or beyond ?


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: grain to grain transfers ? [Re: toreachnirvana]
    #1497624 - 04/27/03 12:10 PM (15 years, 30 days ago)

In TMC, the author demonstrates turning 1 grain jar into 100 using g2g transfers, so 8 jars should give you hmmm, lets see, about 800.  :smile: 


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinetoreachnirvana
newbie
Registered: 03/06/03
Posts: 61
Last seen: 12 years, 6 months
Re: grain to grain transfers ? [Re: dog]
    #1497637 - 04/27/03 12:13 PM (15 years, 30 days ago)

thanks so there is no degeneration to the myc then ? that sounds good to me


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OfflineMike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 5 months, 28 days
Re: grain to grain transfers ? [Re: dog]
    #1497645 - 04/27/03 12:18 PM (15 years, 30 days ago)


Dog

I was searching also for a documented recommended limit for generations produced by myc transfer or cloning, until it would be advisable to break that up with a generation of multispore innoc......do you know ?


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your inside is out, and your outside is in.


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: grain to grain transfers ? [Re: toreachnirvana]
    #1497680 - 04/27/03 12:37 PM (15 years, 30 days ago)

Quote:

thanks so there is no degeneration to the myc then ? that sounds good to me




There is degradation after the third generation according to TMC. In his demonstration he used a petri to inoculate 10 jars, these 10 jars to inoculate 100 more via g2g, then those 100 to do 1000.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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OfflineMike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 5 months, 28 days
Re: grain to grain transfers ? [Re: dog]
    #1499074 - 04/27/03 11:46 PM (15 years, 30 days ago)

Do you know how the degradation expresses itself?

I'm certainly willing to believe Stamets regarding the three generations..... but I wonder what characteristics the mycelium.....or fruits....show to demonstrate that there is a negative direction?


--------------------
your inside is out, and your outside is in.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: grain to grain transfers ? [Re: toreachnirvana]
    #1499108 - 04/28/03 12:01 AM (15 years, 30 days ago)

My foaf goes out 4 generations, with no discernable ill effect.

From G1, he marks, dates & holds out & refridgerates several jars, to stall growth.

Same for G2 & G3.

At G4, while cropping out, he goes back to the G1 stored jars & G2G's them into more jars. So on & so forth, with each generation, until about 1 year has past & the G trail is about to end.

He then goes back to a G1 print & repeats the process.

In other woirds - IMHO, with thought, you can go out about one year from day 1 of the 1st muli/spore innoc if running crop after crop.

LOL, if you have multiple strains, you need a stand alone -- dedicated fridge (or 2), for jar culture storage. Hint. Seal jars tightly, otherwise they will dehydrate.

6T :wink:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineMike Elium
.the Mycelium

Registered: 02/04/03
Posts: 245
Loc: on the edge
Last seen: 5 months, 28 days
Re: grain to grain transfers ? [Re: SixTango]
    #1499190 - 04/28/03 12:22 AM (15 years, 30 days ago)


6T, please also see my post above.....how does the degradation show itself?


--------------------
your inside is out, and your outside is in.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: grain to grain transfers ? [Re: Mike Elium]
    #1499244 - 04/28/03 12:39 AM (15 years, 30 days ago)

Quote:

6T, please also see my post above.....how does the degradation show itself?




What my foaf has seen was that, In a constant controled enviroment, it will demo itself as less rizo, slower substrate colonization, dwarfs, mutants & far less yeild.

6T


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineNewSpore
Just Starting toSee the Light

Registered: 03/26/03
Posts: 467
Last seen: 8 years, 8 months
Re: grain to grain transfers ? [Re: SixTango]
    #1538787 - 05/11/03 11:43 AM (15 years, 16 days ago)

Hey Six, what would you consider slow substrate colonization? And if mustants are present should a friend assume that he has spores from generations past the "Proper Limit" ............... If that were true how does anyone really know what they are getting?


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Be in truth and watch the magic happen.
SBP TEK


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: grain to grain transfers ? [Re: NewSpore]
    #1538806 - 05/11/03 12:05 PM (15 years, 16 days ago)

Spores don't count. They are pretty random, though, so who knows what you're getting? Multispore culture is ok, IMO, because a certain dominant strain will take over.

There can be substrains with some wierd idiosincrasies, though. This is why cloning helps.

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Amazon Shop: ½ Pint Jars, Dehydrator, Paul Stamets, Petri Dish, Rye Grain

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