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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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grain to grain transfers ?
    #1497558 -

how many " generations " can you go when transfering myc from rye to rye. can one say turn 8 jars rye to 128 or beyond ?

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Re: grain to grain transfers ? [Re: toreachnirvana]
    #1497624 -

In TMC, the author demonstrates turning 1 grain jar into 100 using g2g transfers, so 8 jars should give you hmmm, lets see, about 800.  :smile: 


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Re: grain to grain transfers ? [Re: dog]
    #1497637 -

thanks so there is no degeneration to the myc then ? that sounds good to me

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Re: grain to grain transfers ? [Re: dog]
    #1497645 -


Dog

I was searching also for a documented recommended limit for generations produced by myc transfer or cloning, until it would be advisable to break that up with a generation of multispore innoc......do you know ?


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your inside is out, and your outside is in.

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Re: grain to grain transfers ? [Re: toreachnirvana]
    #1497680 -

Quote:
thanks so there is no degeneration to the myc then ? that sounds good to me



There is degradation after the third generation according to TMC. In his demonstration he used a petri to inoculate 10 jars, these 10 jars to inoculate 100 more via g2g, then those 100 to do 1000.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Re: grain to grain transfers ? [Re: dog]
    #1499074 -

Do you know how the degradation expresses itself?

I'm certainly willing to believe Stamets regarding the three generations..... but I wonder what characteristics the mycelium.....or fruits....show to demonstrate that there is a negative direction?


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your inside is out, and your outside is in.

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Re: grain to grain transfers ? [Re: toreachnirvana]
    #1499108 -

My foaf goes out 4 generations, with no discernable ill effect.

From G1, he marks, dates & holds out & refridgerates several jars, to stall growth.

Same for G2 & G3.

At G4, while cropping out, he goes back to the G1 stored jars & G2G's them into more jars. So on & so forth, with each generation, until about 1 year has past & the G trail is about to end.

He then goes back to a G1 print & repeats the process.

In other woirds - IMHO, with thought, you can go out about one year from day 1 of the 1st muli/spore innoc if running crop after crop.

LOL, if you have multiple strains, you need a stand alone -- dedicated fridge (or 2), for jar culture storage. Hint. Seal jars tightly, otherwise they will dehydrate.

6T :wink:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

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Re: grain to grain transfers ? [Re: SixTango]
    #1499190 -


6T, please also see my post above.....how does the degradation show itself?


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your inside is out, and your outside is in.

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Re: grain to grain transfers ? [Re: Mike Elium]
    #1499244 -

Quote:
6T, please also see my post above.....how does the degradation show itself?


What my foaf has seen was that, In a constant controled enviroment, it will demo itself as less rizo, slower substrate colonization, dwarfs, mutants & far less yeild.

6T


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

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Re: grain to grain transfers ? [Re: SixTango]
    #1538787 -

Hey Six, what would you consider slow substrate colonization? And if mustants are present should a friend assume that he has spores from generations past the "Proper Limit" ............... If that were true how does anyone really know what they are getting?


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Be in truth and watch the magic happen.
SBP TEK

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Re: grain to grain transfers ? [Re: NewSpore]
    #1538806 -

Spores don't count. They are pretty random, though, so who knows what you're getting? Multispore culture is ok, IMO, because a certain dominant strain will take over.

There can be substrains with some wierd idiosincrasies, though. This is why cloning helps.

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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