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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Liquid Growth Medium Question
    #1387597 -

I was wondering how much experience any of you have with liquid mycelium growth such as this tek:

http://www.shroomery.org/index/par/7996

What I'm trying to find out is if the yields from growing shrooms this way are greater than the normal BRF/Verm cakes?

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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1387641 -

kramer says yeilds always depend on type of substrate strain and growing conditions.kramer thinks your confusing agar isolates


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KRAMER CAKES



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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1387652 -

Liquid culture is a good way to stretch out a limited spore supply. It also speeds up colonization a bit.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Re: Liquid Growth Medium Question [Re: dog]
    #1387798 -

How long does it take before the mycelium in the liquid is ready to be put in the substrate? Also, do you need to sterilize the substrate when you put the liquid mycelium culture in it, or is that not neccesary since the mycelium is already grown?

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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1387924 -

Everything should be treated exactly as it would be if inoculating from spores.
Liquid cultures usually take between 1-3 weeks to be ready to use.


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"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson

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Re: Liquid Growth Medium Question [Re: z@z.com]
    #1388243 -

It takes 1-3 weeks before the liquid culture is ready to be put in the substrate, or the substrate will be ready to fruit in 1-3 weeks when using liquid agar culture after it has been inoculated?

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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1388334 -

Quote:
It takes 1-3 weeks before the liquid culture is ready to be put in the substrate, or the substrate will be ready to fruit in 1-3 weeks when using liquid agar culture after it has been inoculated?


It will take one to three weeks for the mycelium in the syringe to multiply to an extent that you will be able to innoculate a batch of jars.


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Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html

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Re: Liquid Growth Medium Question [Re: BigJohnson]
    #1389006 -

afoaf has a karo jar incubating @ 84F for nearly 1 week now. Showing growth in two areas that kind of looks like pollen but is a light-gray color.

Edited by Twista (03/18/03 04:59 PM)

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Re: Liquid Growth Medium Question [Re: Twista]
    #1395302 -

If you inoculate jars with the liquid mycelium culture, how many cc's should you use?

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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1395334 -

can you innoculate the liquid with a spore print? same way as the cakes? or works best with syringes?


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Denarse is Dillusional and speaks nonsense. Do not take anything he says Seriously.

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Re: Liquid Growth Medium Question [Re: denarse]
    #1395471 -

Twista - mycelium usually shows up grey-ish or off-white because it's so thin that far into colonization.

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Re: Liquid Growth Medium Question [Re: B4rK0d3]
    #1397796 -

Bump

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Re: Liquid Growth Medium Question [Re: kjb1891]
    #1397816 -

Quote:
If you inoculate jars with the liquid mycelium culture, how many cc's should you use?



One or two cc's from a healthy culture is plenty.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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