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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Questions about Honey TEK
    #471337 -

Ok.. so I didn't quite use Honey TEK.. I used dextrose instead, but it seems to have worked.

I injected 1.5 cc's of my spore syringe into the liquid growth medium, and waited 2 weeks... there are some nice little whiteish strands of mycelium in my jar.. so I put it in the fridge... I figure that 1.5 cc's probably wouldn't create much in the jar.

The question is.. if I went and made another jar, sucked up all the strands from the old jar, and injected them into the new jar... would more be produced then in the original?


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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471408 -

the mycelium will continue to grow until there is no more food source. Also by putting it in the fridge, you are stopping growth, because of the low temp.


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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471557 -

ok.. about to get a bit specific here...

I have a 250 ml jar with the liquid growth solution in it.. and 1 cc out of my cubie spore syringe, questions follow:

1. how much mycelium should the jar have in it, after say two weeks?

2. how long should I let the mycelium grow in the jar before I toss it in the fridge? (cause I won't need to use it for a little while)

3. how many jars can I innoculate with the above jar?

4. when drawing the mycelium into the syringe, should I be only trying to suck up the strands, or will the surrounding solution also cause growth in brf/v substrate?

Any help would be greatly appreciated!! :)

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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471564 -

ugh... one other question :)

the Honey TEK page says I can use any part of living mycelial tissue to start a liquid growth medium on it's way.. does this mean I can just chop a piece of living mushroom off and toss it in the solution and I'll see results? If not, what exactly do I use...

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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471596 -

1. hard to answer. depends upon temperature, food for the mycelium, and the type of strain or characteristics of isolate(if a clone)

2. I'd suggest waiting until you have a nice size cloud. I know that's not telling much, but its the way dude judges. BTW - when you put it in the fridge, id recommend covering the jatr completely with al foil and putting a date on the foil for reference.

3. Hard to judge. you can use 2-3 ml per quart of grain, or 1-2 cc per half pint... really depends.

4. Just shake the jar really well, and then suck into your syringe(youll be sure to get a bunch of mycelium)

5. yes you can clone from a piece of mushroom. dude peels the stem back to the cap and cores the mushroom with a sterile syringe then shoots this into the jar of h.w.


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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471636 -

hmm... interesting.. food for thought, and for mushies :)

I only put 1 cc in the jar tho.. will this be enough to make a "nice size cloud" of mycelium?

Edited by Hammerloaf (11/27/01 08:28 PM)

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Re: Questions about Honey TEK [Re: Hammerloaf]
    #471640 -

Certainly, You don't even need that much...


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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