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OfflineBelovedLaughter
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cobweb mold infestation
    #12303348 - 03/31/10 01:33 AM (13 years, 10 months ago)

Man,  if I were trying to grow bulk trays of cobweb mold,  I'd be really successful.  It's contaminating about 40% of my trays.  I tried hydrogen peroxide;  catchin' it early and sprayin' with 3% solution,  but it's not really workin' for me.  And I don't like the idea of stunting the mycelium.

I'm at the point of asking myself; 

"How much of a problem is this cobweb mold really going to present for me?"

Maybe I should just let the mycelium fend for itself? 

After I realized the dilemmas of too moist of a substrate,  I made my latter trays pretty dry.  This is working very well for me.  They are colonizing quite rapidly so far.

Again however,  the real question I'm asking here is,  what do I really have to worry about?


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InvisibleSmartweed
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Re: cobweb mold infestation [Re: BelovedLaughter]
    #12303382 - 03/31/10 01:40 AM (13 years, 10 months ago)

Bro I suggest that you clean your area where you store your trays while incubating. Empty out everything and clean the whole area with 409 a bunch of times and spray tons of oust. I also suggest that you do the transfer of the spawn to bulk in a glovebox or turn off your AC for atleast 10 minutes, spray the whole area with Oust like crazy.

Also make sure the trays/tubs your spawning to are cleaned correctly, I use 409 and then wipe it down with alcohol. Then I put a black trashbag lining theinside of the tray.

Good luck man.


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OfflineSpongiform
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Re: cobweb mold infestation [Re: Smartweed]
    #12303416 - 03/31/10 01:52 AM (13 years, 10 months ago)

Hydrogen Peroxide will rape cobweb.  I had a batch of sub get contaminated with it once.  Checked on my tub about 4 or 5 days in and it had atleast a 3 inch solid layer of cobweb in it. I couldn't even see the substrate.    I sprayed it down with h202 twice over a 4 hour period.  Next day it was gone and the mycelium was going wild in there. 

If h202 isn't solving your cobweb issue then you might be dealing with a different contam.  Also, make sure you don't try to dilute the h202, use it straight out of the bottle and make sure it's a fresh bottle.  They get weak pretty fast.


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InvisibleSmartweed
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Re: cobweb mold infestation [Re: Spongiform]
    #12303436 - 03/31/10 01:58 AM (13 years, 10 months ago)

Quote:

Spongiform said:
Hydrogen Peroxide will rape cobweb.  I had a batch of sub get contaminated with it once.  Checked on my tub about 4 or 5 days in and it had atleast a 3 inch solid layer of cobweb in it. I couldn't even see the substrate.    I sprayed it down with h202 twice over a 4 hour period.  Next day it was gone and the mycelium was going wild in there. 

If h202 isn't solving your cobweb issue then you might be dealing with a different contam.  Also, make sure you don't try to dilute the h202, use it straight out of the bottle and make sure it's a fresh bottle.  They get weak pretty fast.




ya don't dilute the h202, pour it in your spray bottle and go at it.


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Invisiblebiologys
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Re: cobweb mold infestation [Re: Smartweed]
    #12303451 - 03/31/10 02:04 AM (13 years, 10 months ago)

also the main cause of cobweb is stale stagnet air..if your getting good FAE you shouldn't have aproblem with cobweb.

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OfflineBelovedLaughter
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Re: cobweb mold infestation [Re: biologys]
    #12303480 - 03/31/10 02:18 AM (13 years, 10 months ago)

Yeah,  I hear y'all.  One of the problems is that the trays are incubating,  so I can't introduce FAE because I don't want to induce pinning.

And I keep the incubation area very clean,  so I think my solution in reference to that is to be much more sterile when spawning to bulk,  next time.  That's most likely where I ran into contams.  Transferring the grain spawn to the bulk sub.

Thanks for all the words of wisdom so far everybody.

Any body know how vinegar in combination with peroxide affects mycelium?


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OfflineFahkface
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Re: cobweb mold infestation [Re: BelovedLaughter]
    #12303644 - 03/31/10 04:10 AM (13 years, 10 months ago)

Quote:

Any body know how vinegar in combination with peroxide affects mycelium?




Probably not.

What substrate are you spawning to? Do you pasteurize the substrate`?

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OfflineBelovedLaughter
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Re: cobweb mold infestation [Re: Fahkface]
    #12303663 - 03/31/10 04:26 AM (13 years, 10 months ago)

The substrate I'm using is a blend of manure and spent coffee grounds,  blended with worm castings,  vermiculite,  perlite,  gypsum,  and a little bone meal and blood meal.

I did pasteurize meticulously.  165 o F for an hour.


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OfflineFahkface
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Re: cobweb mold infestation [Re: BelovedLaughter]
    #12303668 - 03/31/10 04:29 AM (13 years, 10 months ago)

Then it was probably your spawn that was infected in the first place. Though, from time to time pasteurized substrates contam as well.

Just try to increase your sterility work, when spawning and do as the others have suggested.

In addition you should probably try to pasteurize a little longer. Like 1,5 to 2 hours.

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OfflineBelovedLaughter
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Re: cobweb mold infestation [Re: Fahkface]
    #12303692 - 03/31/10 04:58 AM (13 years, 10 months ago)

Much appreciated.


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OfflineRogerRabbitM
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Re: cobweb mold infestation [Re: BelovedLaughter]
    #12304392 - 03/31/10 09:48 AM (13 years, 10 months ago)

Quote:

BelovedLaughter said:
Yeah,  I hear y'all.  One of the problems is that the trays are incubating,  so I can't introduce FAE because I don't want to induce pinning.





If you're getting mold during colonization, toss it out.  It means either your spawn was contaminated or you didn't follow proper sterile procedure.  Peroxide is to be used when a small patch of cobweb shows up during the fruiting stage.
RR


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OfflineKryptik
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Re: cobweb mold infestation [Re: RogerRabbit]
    #12304918 - 03/31/10 11:28 AM (13 years, 10 months ago)

I will second the issue with weak peroxide. Make sure you have a fresh bottle when you go after cobweb.

I would take a look at your surrounding setup. Lots of carpet? Old filter in the furnace? Fan in the room...so on.

Along with what RR said, what kind of procedures are you using to sterilize your spawn?


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OfflineBelovedLaughter
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Re: cobweb mold infestation [Re: RogerRabbit]
    #12306295 - 03/31/10 02:52 PM (13 years, 10 months ago)

Thank you RR.  Now,  I've read some threads that have misdiagnosed mycelium as cobweb,  so I want to be sure.  I suppose I'll post some pics in the contamination forum.  DANG though!  That's a grip of my work to toss out.

I strongly feel that my grain spawn was as healthy as can be.  And as this was the first bulk grow I've done,  I wasn't sure how much sterile procedure had to be put into this step of the process.  I inoculated my bulk sub in my kitchen,  with no face mask,  or gloves.  With that being said,  I think it's a safe bet that that's where the sterile procedure was compromised and the contaminates were introduced.

I know that you command a lot of respect RR,  so I have to execute your advice and toss the contaminated trays,  but what if I don't?  What if I was able to isolate them and fruit them?  Afterall,  it's only cobweb mold.


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InvisibleLucid_Euphoria
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Re: cobweb mold infestation [Re: BelovedLaughter]
    #12306367 - 03/31/10 03:04 PM (13 years, 10 months ago)

Quote:

I inoculated my bulk sub in my kitchen,  with no face mask,  or gloves.  With that being said,  I think it's a safe bet that that's where the sterile procedure was compromised and the contaminates were introduced.




:thumbup: You probably hit the nail on the head. Most peoples kitchens are almost as filthy as bathrooms when it comes to micro organisms.

Try to do it in another room. And make sure you wear mask, gloves, etc.

You want as still of air as possible when mixing your spawn, so shut off any fans, air filters, ac, etc.

Also try to work quickly. The less time your substrate is exposed to open air, the less likely you are to introduce contaminate spores, etc.


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