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myceleus_rex
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Registered: 07/01/09
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First go at syringe making... How's this sound?
#11215383 - 10/09/09 01:17 PM (14 years, 5 months ago) |
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This is with a nice dark Burma about 1.5" across. Thanks just_me and uncle
I figure I might as well do up the whole print and that I should be able to get five x 12ml. I plan to boil DH2O in a saucepan with a little KY jelly to limit clumping. I'll then pull it into a new 60ml sterile-packed syringe. This will be recapped and placed in a ziplock bag sprayed with lysol and allowed to cool. In a still-air box I'll transfer the spores to a small glass dish, wiped with alcohol shortly before, along with the 60ml of DH2O. I plan to suck it back in the 60ml syringe and squirt it back out to get a good mix. I then fill five new 12ml syringes as 5x12=60.
How does this sound, people?
I'm also wondering if I can rinse the spores off the foil with the water from the 60ml or if I should scrape them off.
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noobieshroomie
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215414 - 10/09/09 01:21 PM (14 years, 5 months ago) |
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you dont want to use the whole print a tiny corner is more that enough to make a syringe
that print is not 100% sterile and you would be mixing all the contams pu if you use the whole thing
possibly contaminating every syringe you make
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215426 - 10/09/09 01:22 PM (14 years, 5 months ago) |
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Less is more
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drift
Registered: 01/29/09
Posts: 1,274
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215439 - 10/09/09 01:24 PM (14 years, 5 months ago) |
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Quote:
myceleus_rex said:I'm also wondering if I can rinse the spores off the foil with the water from the 60ml or if I should scrape them off.
Scrape them off, and listen to what the other guys said about not using the whole syringe.
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myceleus_rex
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Re: First go at syringe making... How's this sound? [Re: noobieshroomie]
#11215469 - 10/09/09 01:30 PM (14 years, 5 months ago) |
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Thanks for the input. My idea was inspired by this:
Magash's Syringe Tek
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shx764
That Guy
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215483 - 10/09/09 01:32 PM (14 years, 5 months ago) |
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Sounds like you have a good sterile procedure planned.
-------------------- All labor that uplifts humanity has dignity and importance and should be undertaken with painstaking excellence. - Martin Luther King, Jr.
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SA5M
Rapid Colonizer
Registered: 08/12/09
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215538 - 10/09/09 01:39 PM (14 years, 5 months ago) |
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I also made my first endeavor into syringe making. I followed Magash's tek as well. I had a few questions about it myself here. If you follow the tek and use a glovebox there is nothing to it. Personally I like dark needles, and from 1 3" diameter print I did 3 needles. I probably could have done 20 or more with this print, and will adjust accordingly next time.
On the subject of dark needles tho, I had a Tex syringe that was as dark as night and from that my 1/2 pint PF inoculated jars were colonized %100 in 6 days.
There is something to be said about making your own needles. ;D
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13shrooms
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11215598 - 10/09/09 01:48 PM (14 years, 5 months ago) |
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inside your glovebox put a sterile shotglass your print, and a couple of syringes already filled with sterile water and whatever tool you use for scraping your spores.
scrape about a 1/8" square off into the glass then squirt your syringe water in the glass and swirl with needle still in the water and fill your syringe back up. repeat as needed.
pending how long ago/fresh your print is you can use right away or let sit 4 a day to rehydrate your spores.
13
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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noobieshroomie
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Re: First go at syringe making... How's this sound? [Re: 13shrooms]
#11215615 - 10/09/09 01:52 PM (14 years, 5 months ago) |
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thats the way i make mine easy and effective
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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myceleus_rex
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Re: First go at syringe making... How's this sound? [Re: noobieshroomie]
#11215727 - 10/09/09 02:08 PM (14 years, 5 months ago) |
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I'm glad I posted
I hadn't considered that using all the spores at once might increase contamination odds, although that now seems obvious. No way I need five stick worth right now. Just one is probably the way to go.
Do you use the syringe needle to scrape the spores? I don't have an inoculation loupe an the needle will already be sterile.
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SA5M
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11216588 - 10/09/09 04:55 PM (14 years, 5 months ago) |
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I used the needle tip. From further investigation you can damage the spores from doing this, using the sharp needle tip to scrape them in the shot glass.
The best suggestion I have read; if you don't have an innocu loop, is to use a Japanese ear cleaner. It is long w/ soft round edges, they come in plastic and wood. I had one, but didn't read this until I already made the needles.
Using the tips turned out fine, just food for thought since you asked.
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: First go at syringe making... How's this sound? [Re: SA5M]
#11216593 - 10/09/09 04:56 PM (14 years, 5 months ago) |
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Using a the tip of a syringe is fine damaging a few spores wont really matter since there TONNES of them in a syringe even if you cant see lots of them(which is what you want)
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myceleus_rex
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Re: First go at syringe making... How's this sound? [Re: SA5M]
#11216705 - 10/09/09 05:18 PM (14 years, 5 months ago) |
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Quote:
SA5M said: I used the needle tip. From further investigation you can damage the spores from doing this, using the sharp needle tip to scrape them in the shot glass.
The best suggestion I have read; if you don't have an innocu loop, is to use a Japanese ear cleaner. It is long w/ soft round edges, they come in plastic and wood. I had one, but didn't read this until I already made the needles.
Using the tips turned out fine, just food for thought since you asked.
I have to ask: What the hell is a Japanese ear cleaner, and do they only clean Japanese ears?
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noobieshroomie
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11216757 - 10/09/09 05:30 PM (14 years, 5 months ago) |
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its likely you will kill a few of them
but whats 1000 when you still have ten thousand left in the syringe
you will be ok for now but it will be well worth it to pick one up
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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po0dingles
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Registered: 08/20/09
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Re: First go at syringe making... How's this sound? *DELETED* [Re: myceleus_rex]
#11216799 - 10/09/09 05:36 PM (14 years, 5 months ago) |
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Post deleted by po0dinglesReason for deletion: X
Edited by po0dingles (10/09/09 05:37 PM)
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myceleus_rex
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Re: First go at syringe making... How's this sound? [Re: po0dingles]
#11234076 - 10/12/09 03:56 PM (14 years, 5 months ago) |
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Well, I did the deed. I dusted this off out of the old posts:
Syringe Tek
It sounded silly at first, but the more I thought about it, the more I liked, and at least one TC is down with it.
Seemed to go well and the syringe looks good. We'll see how it works out.
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m00nshine
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11234088 - 10/12/09 03:59 PM (14 years, 5 months ago) |
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Easy way to make syringes.
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myceleus_rex
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Re: First go at syringe making... How's this sound? [Re: m00nshine]
#11234173 - 10/12/09 04:16 PM (14 years, 5 months ago) |
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Yeah, I've seen that type tek and it looks good, but I didn't make the print. It was on foil and I only made one stick. I don't think I've seen that exact thread though and it looks to have some good stuff. Thanks
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Goat Boy
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Re: First go at syringe making... How's this sound? [Re: myceleus_rex]
#11235342 - 10/12/09 07:42 PM (14 years, 5 months ago) |
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Not to hijack your thread but how many cc's should be injected into a pint jar?
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13shrooms
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Re: First go at syringe making... How's this sound? [Re: Goat Boy]
#11235371 - 10/12/09 07:48 PM (14 years, 5 months ago) |
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1cc is used in a 1/2 pint and 1cc - 2cc is used in 1 quart jars so anywhere in between is good.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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