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Invisibleagar
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Registered: 11/21/04
Posts: 9,056
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GRAIN LC * 13
    #6817707 - 04/22/07 01:13 AM (16 years, 9 months ago)



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Edited by agar (04/22/07 01:53 AM)


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Invisibledutchmushroom
mushroom invader
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Registered: 08/02/06
Posts: 1,393
Loc: Outer Space
Re: GRAIN LC [Re: agar]
    #6817999 - 04/22/07 04:01 AM (16 years, 9 months ago)

very nice agar

peace out


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"Comes a time when the blind man takes your hand says: don't you see? Gotta make it some how, on the dreams you still believe, Don't give it up, you've got an empty cup, only love can fill, only love can fill" < Grateful Dead!

The bus came by and I got on, and thats when it all began


GROWLIGHT KIT, 250w HPS Digital ballast, + Enhanced Spectrum bulb and Reflector Sale Or Trade!
     


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Invisiblemonstermitch
Growing in Bags Doesn't Work


Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: GRAIN LC [Re: dutchmushroom] * 2
    #6885786 - 05/07/07 06:23 PM (16 years, 8 months ago)

Quote:

agar said:

Pint jar on right is multispore inoculated (SA).
Allowed to colonize near 100%.
Syringe is 60 ML w/18 gauge needle.
Quart jar on left is sterilized water.

Aspirated 60 ML sterile water into syringe.

Injected 60 ML sterile water into colonized grain jar.

Shook grain jar to liberate mycelium from grain.

Injected total of 180 ML sterile water into grain jar.
(shaking mildly between each injection)

Aspirated out mycelium laden water from grain jar.
(all I could aspirate out of the grain jar was 150 ML)
Then, injected mycelium laden water back into sterile water jar.

Approximately 500 ML of Mycelium laden water. :rockon:
(enough LC for approximately 50....10 ML syringes)

The advantage of this procedure is that you can be assured.
The culture in clean.:grin:
Because you can VISUALLY inspect colonization in the grain jar.
(if there is FUNK, you can see it :rolleyes:)

That is not the case, with sugar type LC's.
(multispore syringe inoculated)
They can be contaminated, from day 1.
And, you NEVER know it. :frown:
Until you inoculate spawn jars with that LC.
Then, have every single spawn jar GO SOUTH. :eek: :mad2:





just so the newer guys, whom can't see the log section, can see it .


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Offlineshrooms4unme
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Registered: 10/05/06
Posts: 47
Last seen: 16 years, 6 months
Re: GRAIN LC [Re: monstermitch]
    #6885987 - 05/07/07 07:20 PM (16 years, 8 months ago)

Should I make the injection port on the side of the lid when I make these?

I was thinking about making new lids to use for this tek, because it's hard as hell to aspirate liquid from a port that's in the center of the lid.

Do you guys think this would be a good idea if i tweaked my lids?


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OfflineAkira
CosmicConsciousness
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Registered: 12/30/05
Posts: 2,283
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Re: GRAIN LC [Re: shrooms4unme] * 1
    #6886082 - 05/07/07 07:41 PM (16 years, 8 months ago)

Damn that is very creative agar. Much respect on your craftiness.


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Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: GRAIN LC [Re: Akira]
    #6886318 - 05/07/07 08:32 PM (16 years, 8 months ago)

i second that thought. off to my fav's


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OfflineTheFish
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Re: GRAIN LC [Re: blood4blood]
    #6886413 - 05/07/07 08:47 PM (16 years, 8 months ago)

I've used this technique before, and I find that my colonization of new grains from a water based LC has taken a while to start growth whereas other sugar based LC's have taken 48-72 hours to see vigorous growth.

Any idea as to why? I have been using Creepers.


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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InvisiblefahtsterM
Now With 33%More Faht
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Registered: 06/17/06
Posts: 9,266
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Re: GRAIN LC [Re: Akira]
    #6886752 - 05/07/07 09:51 PM (16 years, 8 months ago)

this is a great tek... I've been doing it for years. :wink:  you can use it to clone to (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6590986/an/0/page/2)

or you can use the same process to blindly reduce the number of substrains present in a MS (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5781218/an/0/page/3

you can also dry out those grain jars and store at room temp. to reanimate for later use... you need a filter controlling lid tho.. (http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6886717/an/0/page/0)

nice extension of the tek, Agar. :thumbup:

Quote:

...Much respect on your craftiness.




thank you

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6603066/an/0/page/0

fahtster


Edited by fahtster (05/07/07 10:18 PM)


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OfflineSnaggletooth
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Registered: 10/24/05
Posts: 6,109
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Re: GRAIN LC [Re: fahtster]
    #6886832 - 05/07/07 10:05 PM (16 years, 8 months ago)






Next week Agar tackles Quantum Mechanics....the theory of everything!


HA! very cool man, never even thought about that. Damn nice!


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Atheist Chat


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Invisibleagar
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Re: GRAIN LC [Re: Snaggletooth]
    #6886881 - 05/07/07 10:14 PM (16 years, 8 months ago)


LOL "agar's" LSD rig


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Invisiblebudmanman
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Registered: 02/07/07
Posts: 17,974
Loc: PNW
Re: GRAIN LC [Re: agar]
    #6886943 - 05/07/07 10:24 PM (16 years, 8 months ago)

I wish I knew were to get those lids at =X


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Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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OfflineSnaggletooth
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Re: GRAIN LC [Re: agar]
    #6886982 - 05/07/07 10:28 PM (16 years, 8 months ago)

So you did retain some of that college experience from the 60's :wink:





It great seeing someone that has a genuine interest as to pass the info along, teachers learn much more then students :cheers:


--------------------


Atheist Chat


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InvisiblefahtsterM
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Registered: 06/17/06
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Re: GRAIN LC *DELETED* [Re: Snaggletooth]
    #6887074 - 05/07/07 10:42 PM (16 years, 8 months ago)

Off topic post removed to save the thread.


Edited by RogerRabbit (05/09/07 08:37 AM)


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OfflineSnaggletooth
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Re: GRAIN LC [Re: fahtster]
    #6887121 - 05/07/07 10:52 PM (16 years, 8 months ago)

Off topic post removed to save the thread.


Edited by RogerRabbit (05/09/07 08:36 AM)


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Offlineand0rr
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Re: GRAIN LC [Re: fahtster]
    #6887146 - 05/07/07 10:56 PM (16 years, 8 months ago)

Off topic post removed to save the thread.


--------------------
"Our society is run by insane people for insane objectives. I think we're being run by maniacs for maniacal ends and I think I'm liable to be put away as insane for expressing that. That's what's insane about it." -John Lennon


Edited by RogerRabbit (05/09/07 08:37 AM)


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InvisiblefahtsterM
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Registered: 06/17/06
Posts: 9,266
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Re: GRAIN LC [Re: Snaggletooth]
    #6887152 - 05/07/07 10:57 PM (16 years, 8 months ago)

Off topic post removed to save the thread.


Edited by RogerRabbit (05/09/07 08:37 AM)


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Invisibleagar
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Re: GRAIN LC [Re: fahtster]
    #6888313 - 05/08/07 08:54 AM (16 years, 8 months ago)

Sterile water was first used to preserve cultures by Castellani in 1939 (2). Since then, many scientists have used this method; McGinnis et al. in 1974 (3) and Odds in 1991 (4) reported that they were able to maintain viable cultures for more than three years, without degredation.

(2) S.Castellani,"Viability of Some Pathogenic Fungi in Distilled Water", Journal of Tropical Medicine and Hygiene 42, pp 225-226 (1939)

(3) M.R.McGinnis,A.A.Padhye,and L. Ajello,"Storage of Stock Cultures of Filamentous Fungi,Yeasts, and Some Aerobic Actinomycetes in Sterile Distilled Water", Applied Microbiology 28, pp 218-222 (1974)

(4) F.C.Odds, "Long Term Laboratory Preservation of Pathogenic Yeast in Water",Journal of Medical and Veterinary Mycology 29, pp 413-415(1991)


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InvisiblefahtsterM
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Re: GRAIN LC [Re: agar]
    #6888831 - 05/08/07 11:36 AM (16 years, 8 months ago)

Off topic post removed to save the thread.


Edited by RogerRabbit (05/09/07 08:38 AM)


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OfflineTheFish
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Registered: 10/07/03
Posts: 440
Last seen: 8 years, 6 months
Re: GRAIN LC [Re: TheFish]
    #6888951 - 05/08/07 12:25 PM (16 years, 8 months ago)

Quote:

TheFish said:
I've used this technique before, and I find that my colonization of new grains from a water based LC has taken a while to start growth whereas other sugar based LC's have taken 48-72 hours to see vigorous growth.

Any idea as to why? I have been using Creepers.




--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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Invisiblebudmanman
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Male


Registered: 02/07/07
Posts: 17,974
Loc: PNW
Re: GRAIN LC [Re: TheFish]
    #6889062 - 05/08/07 01:15 PM (16 years, 8 months ago)

I am cerial were do get those lids.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.


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