Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePoppaWoody
member
Registered: 06/12/01
Posts: 112
Loc: Tampa, Fl
Last seen: 22 years, 23 days
First time trying to make syringe from print
    #386221 - 09/05/01 10:12 AM (22 years, 5 months ago)

Anyone have any good links o the teqhnique

Thanks in advance

Remember: Your whole purpose in life may be to serve as a warning to others


--------------------
[red]Remember:[/red] Your whole purpose in life may be to serve as a warning to others

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 2 days, 12 hours
Re: First time trying to make syringe from print [Re: PoppaWoody]
    #386236 - 09/05/01 10:40 AM (22 years, 5 months ago)


Extras: Filter Print Post Top
Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: First time trying to make syringe from print [Re: Anno]
    #386337 - 09/05/01 02:09 PM (22 years, 5 months ago)

Psilocyber's Syringe Tek v1.0

NOTE: These instructions are most effective when performed in the most sterile environment available. The preferred method involves following the steps below while working in a clean and sterile glovebox. There are many simple methods of glovebox construction; most are available on the web at the popular mycological culture websites. If you do not wish to construct a glovebox, or do not have one, the following steps have been performed with success by using the "oven tek." This simply involves using your oven as a way of reducing the amount of contamination present. To do this, turn your oven on its "warm" setting. Pull down the door. You may then use the open door as a working surface or pull out one of the oven racks halfway and work on it. The theory (unproven) is that rising heat from the oven causes airborne contaminates to rise and therefore prevents them from settling on your working surface and therefore reducing the number of contaminates present.

Materials needed:

Empty sterile syringes

Two quart (or larger) saucepan

One bottle of 91% isopropyl alcohol

Several paper towels

A lighter or alcohol flame

A shot glass

Sterile spore print Procedure One: Making a sterile syringe

1. Fill your saucepan halfway with tap or distilled water (use distilled water if your tap water contains higher levels of minerals and chemicals).

2. Boil the water in the saucepan on high for a minimum of ten minutes, this should be adequate to sterilize and cleans the water of all bacteria and viruses.

3. Take your empty syringe and fill it with the boiling water. Allow it to sit for two minutes with the hot water inside.

4. Purge the hot water from the syringe into a sink, not back into the saucepan.

5. Repeat steps 3 and 4 two more times. Upon the second time leave the hot water in the syringe.

6. Place the syringe in a cool draft-free place, preferable in a clean zip-lock bag

7. Allow it to cool for several hours before proceeding to Procedure Two.

Procedure Two: Transferring print spores into syringe

1. First clean your work area. This may involve wiping down all work surfaces with a diluted bleach solution and spraying the area liberally with a disinfectant such as Lysol.

2. Place the following materials in your glovebox or on the oven door working surface: The shot glass, your cooled syringes, the bottle of alcohol, a paper towel, your print (still in zip-lock baggie) and the lighter or alcohol flame.

3. Wash hands with antibacterial soap before proceeding further.

4. Fold the paper towel up into ? sections and soak a corner of it with the alcohol.

5. With the alcohol soaked towel wipe the interior of the shot glass, essentially sterilizing the surface you are about to use in the transfer. Allow the shot glass to air dry, should only take a few seconds.

6. Remove the needle guard from your sterile syringe and flame sterilize the needle. Then take your alcohol soaked paper towel and wipe the needle with it to further aid in the sterilization. At this point try to avoid letting the needle touch any other surface unless otherwise instructed to do so.

7. NOTE: it is important at this point to work as quickly as possible to help combat the chances of contaminating molds and bacteria falling into your work area and thereby ruining your syringe.

8. Remove the print from its storage baggie. Unfold it to expose the spores. Lightly begin to scrape, using the needle of the syringe, a section of the print off into the shot glass. For a medium sized print it is usually adequate to scrape off a section no larger than 1/5 of the total print.

9. You will have a small noticeable collection of spores in the shot glass. Now expunge no more than half of the water from the syringe into the shot glass, lightly stirring the spores into the solution.

10. Suck the spore water solution into the syringe. You may need to expunge some more water into the shot glass and re-suck to help in capturing all the spores into the syringe.

11. Once you have the spore solution back into the syringe you should notice that the water inside has turned a darker shade of color and you may see small clusters of spores floating in the solution. This is good, you have completed the process.

12. Sterilize the needle again with the alcohol soaked paper towel, replace the needle guard and place the syringe back into your clean zip-lock bag.

13. Allow the syringe to sit for no less than 12 hours before using it in jar inoculation. This is extremely important, as the spores must be allowed to rehydrate before they can be introduced into the substrate material. Failure to allow this may result in slow or no germination



--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

Extras: Filter Print Post Top
InvisibleMycovirgin
Stranger


Registered: 09/06/19
Posts: 15
Loc: East Asia
Re: First time trying to make syringe from print [Re: phrozendata]
    #26596465 - 04/12/20 07:13 PM (3 years, 10 months ago)

Is this tek still considered good for making syringes from prints?

I will keep reading, but this is like 18 years old?


--------------------
At the deepest level, our consciousness is what we are—to the extent that if we are not sovereign over our own consciousness then we cannot in any meaningful sense be sovereign over anything else either. _ Graham Hancock

Extras: Filter Print Post Top
InvisibleRoger Clemency
Smile
 User Gallery

Registered: 03/23/20
Posts: 2,005
Re: First time trying to make syringe from print [Re: Mycovirgin]
    #26596532 - 04/12/20 07:43 PM (3 years, 10 months ago)

No there are several mistakes in there and some unnecessaries.

I don’t know a link for a good tek off the top but you never want to flame sterilize something and then wipe it with alcohol. Alcohol sanitizes, white hot flame sterilizes.

On the same tip you would not be sterilizing that shot glass by wiping it with alcohol.

You could PC a half pint jar with some water in it and a home made metal tray for holding a cap. Like use thin gauge wire to make a little rack that hangs down into the jar a bit to sit the cap on. You just have to find a way to put some kind of lid over that setup while PCing and while the cap is sitting in there dropping it’s spores. Then suck them out later in a SAB with a flames needle.

Or PC a half pint (or similar) and then just set an alcohol wiped cap inside it in your SAB and close the lid, once it drops spores pick the cap out with a pin or something and squirt sterilized water in and suck it back up.

THere’s probably a newer better tek around somewhere though


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
InvisibleMycovirgin
Stranger


Registered: 09/06/19
Posts: 15
Loc: East Asia
Re: First time trying to make syringe from print [Re: Roger Clemency]
    #26616783 - 04/21/20 04:18 AM (3 years, 10 months ago)

Thanks for answering, i appreciate your time.


--------------------
At the deepest level, our consciousness is what we are—to the extent that if we are not sovereign over our own consciousness then we cannot in any meaningful sense be sovereign over anything else either. _ Graham Hancock

Extras: Filter Print Post Top
Invisiblewoofwoof
such mushrooms!
I'm a teapot User Gallery


Registered: 01/04/19
Posts: 1,127
Re: First time trying to make syringe from print [Re: Mycovirgin]
    #26616810 - 04/21/20 05:04 AM (3 years, 10 months ago)


Extras: Filter Print Post Top
InvisibleMycovirgin
Stranger


Registered: 09/06/19
Posts: 15
Loc: East Asia
Re: First time trying to make syringe from print [Re: woofwoof]
    #26621160 - 04/22/20 10:25 PM (3 years, 10 months ago)

Quote:

woofwoof said:
Follow this one, it is the latest

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681




Thanks, I will


--------------------
At the deepest level, our consciousness is what we are—to the extent that if we are not sovereign over our own consciousness then we cannot in any meaningful sense be sovereign over anything else either. _ Graham Hancock

Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery

Folding@home Statistics
Registered: 05/12/07
Posts: 69,687
Loc: The Inexpressible... Flag
Last seen: 8 hours, 9 minutes
Trusted Cultivator
Re: First time trying to make syringe from print [Re: PoppaWoody]
    #26621308 - 04/22/20 10:25 PM (3 years, 10 months ago)

This thread has been closed.

Reason:
Old thread. Outdated tek.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* storage of prints XAZIA 4,891 11 01/25/05 12:20 PM
by PlaCoFuGostei
* How do you use spore prints efficently? dnkyrhubarb 20,155 14 03/03/22 04:07 PM
by Schtarker
* I am scared to do a Print/syring!
( 1 2 all )
fearforce7 9,062 27 12/30/21 08:54 AM
by Forrester
* detailed printing tek... auto59009 6,676 10 07/20/01 06:27 PM
by patzee
* Making a Print CaseXX 2,756 7 11/04/01 08:27 PM
by auto59009
* Prints to Syringes CaseXX 1,327 2 11/04/01 08:26 PM
by auto59009
* re-hydration of a print Ganoderma 990 4 09/11/03 10:56 AM
by Ganoderma
* Success rate at first time making a sterile syring tekramrepus 5,502 13 02/20/20 08:59 AM
by InfiniteDreams

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,066 topic views. 21 members, 124 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 12 queries.