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OfflineCaseXX
member

Registered: 09/14/01
Posts: 123
Last seen: 15 years, 1 month
Prints to Syringes
    #445795 - 11/03/01 05:02 PM (15 years, 6 months ago)

What is the best way to take a print and get it too the syringe? I made a print of my cambodians! They took off over a couple of nights ago, I'm loving it.
CaseXX


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CaseXX
"Dirty Deeds Done Dirt Cheap" AC/DC


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Invisible40oz
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Registered: 01/19/01
Posts: 30,038
Loc: Sandy Eggo. Ca.
Re: Prints to Syringes [Re: CaseXX]
    #445838 - 11/03/01 06:02 PM (15 years, 6 months ago)



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Offlineauto59009
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Registered: 05/21/01
Posts: 376
Loc: Australia
Last seen: 15 years, 6 months
Re: Prints to Syringes [Re: 40oz]
    #447088 - 11/04/01 10:26 PM (15 years, 6 months ago)

Introduction:

I have used the following tek to produce countless syringes with a success rate of very nearly 100%. Quite simply, it allows anyone to turn a spore print into a syringe filled with a clean, viable spore solution. It is almost impossible for the home cultivator to achieve a completely sterile working area, so the cultivator must suffice with a RELATIVELY sterile working area. An abundance of spores perpetually permeate the air around us, yet these must be excluded from the syringe if contamination is to be avoided at germination of the mushroom spores.


Equipment:

A dust mask
Latex gloves
Aerosol isospropyl alcohol (Glen20; or Isocol in a spray bottle)
An empty, sterile, syringe
Distilled H20
A coffee mug
Wax paper (or cling wrap)
A microwave
Alcohol swabs (or tissues sprayed with alcohol)
A resealable plastic sandwich bag
A babyfood jar
A pressure cooker (or stovetop and pot)
A spore print


Method:

1. Don the dust mask and a pair of latex gloves. Find a small (preferably at least partially enclosed) surface to work on. Thoroughly wipe down the with alcohol, then spray the entire area liberally. Also spray gloved hands to sterilise.

2. Place syringe(s) on surface. Fill the mug with distilled water and cover with wax paper (cling wrap also works well but tends to explode in the microwave). Place the mug in the microwave and sterilise it. Sterilisation is usually achieved when the water has been bubbling for several minutes.

3. Remove sterilised water from the microwave and bring it to the surface near the syringes. Remove the cap from the syringes and, stabbing the needle through the paper, draw up the boiling water. Wipe down the needle with an alcohol swab and recap quickly. Repeat for each syringe. Seal all syringes in the sandwich bag and place somewhere relatively clean to cool down. The freezer has always worked well for me in the past.

4. Whilst waiting for the syringes to return to room temperature you should empty the babyfood jar and rinse it well. Then place the jar and lid separately in a pressure cooker. It must stay in the boiling water for 30 minutes or so to guarantee sterilisation. With a regular pot full of water, it should boil covered for about 60 minutes.

5. Release the steam from the pressure cooker and remove the lid, or with a pot, just remove the lid. With several layers of latex gloves (3, maybe 4, should suffice) raise the babyfood jar (upside down) just above the water line to empty it. Then quickly screw the lid on it. Leave it somewhere to cool to room temperaure.

6. Once both syringes and jar have cooled bring them to the working surface and repeat Step 1. Place the sterile spore print on the surface. Now, working as quickly as possible (without creating severe) air currents remove the cap of the first syringe, then remove the lid of the babyfood jar, and use the forceful stream of water ejected from the syringe to direct spores from the spore print into the jar.

This method does not always work well with all printing mediums, however, it works excellently with all my foil prints. If it will not work with your print medium there are alternative methods. Fold the print in half and rub the sides together over the jar. Or use a retractable blade type Xacto knife that has been wiped down with an alcohol swab to scrape the spores into the jar. Do not use the whole print if you intend to make more than one syringe.

Squirt the remaining water into the jar. Draw the spore filled water back into the syringe. Wipe down the needle with the alcohol swab and recap. Repeat this process with each new syringe.

Store labelled syringes in a sealed plastic bag in a cool, dry environment, out of direct light.


Notes:

To sterilise previously used syringes disassemble them and boil/pressure cook the pieces, reassemble just above the water line whilst the water is still boiling/hot (use multiple layers of latex gloves to avoid burns).

A babyfood jar is used because it has a small mouth, minimising the risk of airborne contaminants.

Spraying the area with aerosol alcohol throughout the process should minimise airborne contaminants.

Be sure to keep aerosol alcohol, alcohol wipes, flames, heat, etc.. away from spores, this will not only kill bacterial spores but also mushrooms spores!

If you have any suggestions/comments please don't hesitate to email me at auto59009@angelfire.com


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I am a compulsive liar :wink:


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