|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 dnkyrhubarb
Stranger
Registered: 09/03/01
Posts: 18
Last seen: 23 years, 6 months
|
 How do you use spore prints efficently?   2
#385558 - 09/04/01 02:32 PM (23 years, 7 months ago) |
|
|
hello I was wondering what is the most efficent way to use spore prints? Do you scrape a little into a solution for a syringe? How much? And also how do you make a good clean print? Sorry for all the questions but I was just wondering.
FreeSporeRing via the wired
--------------------
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 1 month, 12 days
|
 Re: How do you use spore prints efficently? [Re: dnkyrhubarb] 1
#385597 - 09/04/01 03:46 PM (23 years, 7 months ago) |
|
|
Use http://www.sporeworks.com/syringetek.html for making a syringe.
Making a print: Let you and all your tools be clean and sterilized with propanol.
Cut the cap that has opened and that beginns to drop spores(you see the spores as a purple dust either on the stem or on the cap surface(!) ) off the shroom with a clean knive or scisors.
Place the cap facing the gills down on a clean piece of paper(typing paper fresh out of the box wiorks well, also index cards,glass slides or aluminum foil).
Put a drinking glas over it.
Let sit for 12-24 hours.
Remove the cap.
Package the print in a small plasic bag, or fold it together and stick the sides together with a tape.
Edited by Anno on 09/04/01 04:46 PM. Edited by Anno on 09/04/01 04:47 PM.
|
dnkyrhubarb
Stranger
Registered: 09/03/01
Posts: 18
Last seen: 23 years, 6 months
|
Re: How do you use spore prints efficently? [Re: Anno] 1
#385645 - 09/04/01 04:53 PM (23 years, 7 months ago) |
|
|
thanks for the info. i hope it helps other newbies like me
FreeSporeRing via the wired
--------------------
|
 phrozendata
Carpal Tunnel
Registered: 04/23/00
Posts: 5,015
|
Re: How do you use spore prints efficently? [Re: dnkyrhubarb] 1
#385739 - 09/04/01 07:06 PM (23 years, 7 months ago) |
|
|
You can just cover the cap with a glass. It is important to cover the whole paper with something as to keep it as clean as possible. Moreover, it is always good to let spores drop for a few hours in a sterile area and then move the cap to another sterile area for the real print. This will also aid in sterlization of the print but is not at all necessary.
--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley
|
stoner
journeyman
Registered: 03/01/01
Posts: 71
Last seen: 23 years, 1 month
|
Re: How do you use spore prints efficently? [Re: dnkyrhubarb] 1
#385777 - 09/04/01 07:36 PM (23 years, 7 months ago) |
|
|
how do you make a spore print on paper into a spore syringe? what size syringe should you use and where can you aquire one?
|
phrozendata
Carpal Tunnel
Registered: 04/23/00
Posts: 5,015
|
Re: How do you use spore prints efficently? [Re: stoner] 2
#385930 - 09/04/01 10:00 PM (23 years, 7 months ago) |
|
|
Use 10 or 12CC syringes. Make them with the following tek:
Psilocyber's Syringe Tek v1.0
NOTE: These instructions are most effective when performed in the most sterile environment available. The preferred method involves following the steps below while working in a clean and sterile glovebox. There are many simple methods of glovebox construction; most are available on the web at the popular mycological culture websites. If you do not wish to construct a glovebox, or do not have one, the following steps have been performed with success by using the "oven tek." This simply involves using your oven as a way of reducing the amount of contamination present. To do this, turn your oven on its "warm" setting. Pull down the door. You may then use the open door as a working surface or pull out one of the oven racks halfway and work on it. The theory (unproven) is that rising heat from the oven causes airborne contaminates to rise and therefore prevents them from settling on your working surface and therefore reducing the number of contaminates present.
Materials needed:
Empty sterile syringes
Two quart (or larger) saucepan
One bottle of 91% isopropyl alcohol
Several paper towels
A lighter or alcohol flame
A shot glass
Sterile spore print Procedure One: Making a sterile syringe
1. Fill your saucepan halfway with tap or distilled water (use distilled water if your tap water contains higher levels of minerals and chemicals).
2. Boil the water in the saucepan on high for a minimum of ten minutes, this should be adequate to sterilize and cleans the water of all bacteria and viruses.
3. Take your empty syringe and fill it with the boiling water. Allow it to sit for two minutes with the hot water inside.
4. Purge the hot water from the syringe into a sink, not back into the saucepan.
5. Repeat steps 3 and 4 two more times. Upon the second time leave the hot water in the syringe.
6. Place the syringe in a cool draft-free place, preferable in a clean zip-lock bag
7. Allow it to cool for several hours before proceeding to Procedure Two.
Procedure Two: Transferring print spores into syringe
1. First clean your work area. This may involve wiping down all work surfaces with a diluted bleach solution and spraying the area liberally with a disinfectant such as Lysol.
2. Place the following materials in your glovebox or on the oven door working surface: The shot glass, your cooled syringes, the bottle of alcohol, a paper towel, your print (still in zip-lock baggie) and the lighter or alcohol flame.
3. Wash hands with antibacterial soap before proceeding further.
4. Fold the paper towel up into ? sections and soak a corner of it with the alcohol.
5. With the alcohol soaked towel wipe the interior of the shot glass, essentially sterilizing the surface you are about to use in the transfer. Allow the shot glass to air dry, should only take a few seconds.
6. Remove the needle guard from your sterile syringe and flame sterilize the needle. Then take your alcohol soaked paper towel and wipe the needle with it to further aid in the sterilization. At this point try to avoid letting the needle touch any other surface unless otherwise instructed to do so.
7. NOTE: it is important at this point to work as quickly as possible to help combat the chances of contaminating molds and bacteria falling into your work area and thereby ruining your syringe.
8. Remove the print from its storage baggie. Unfold it to expose the spores. Lightly begin to scrape, using the needle of the syringe, a section of the print off into the shot glass. For a medium sized print it is usually adequate to scrape off a section no larger than 1/5 of the total print.
9. You will have a small noticeable collection of spores in the shot glass. Now expunge no more than half of the water from the syringe into the shot glass, lightly stirring the spores into the solution.
10. Suck the spore water solution into the syringe. You may need to expunge some more water into the shot glass and re-suck to help in capturing all the spores into the syringe.
11. Once you have the spore solution back into the syringe you should notice that the water inside has turned a darker shade of color and you may see small clusters of spores floating in the solution. This is good, you have completed the process.
12. Sterilize the needle again with the alcohol soaked paper towel, replace the needle guard and place the syringe back into your clean zip-lock bag.
13. Allow the syringe to sit for no less than 12 hours before using it in jar inoculation. This is extremely important, as the spores must be allowed to rehydrate before they can be introduced into the substrate material. Failure to allow this may result in slow or no germination
--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley
|
Papa_Bear
M-M GOOD!
Registered: 08/30/01
Posts: 756
Loc: Colorado
Last seen: 5 years, 6 months
|
Re: How do you use spore prints efficently? [Re: dnkyrhubarb] 2
#385962 - 09/04/01 11:02 PM (23 years, 7 months ago) |
|
|
I have used a tech of putting the shroom top directly into a small Sterile jar. Let the spores drop. When this is finished remove the top, add your sterlie water to the jar, depending the size of your jar. Some mixing may be required after the spores are hydrated. Use a sterile stirring rod of any type. You should have a small hole in the lid to suck up the solution into your syringe and there you are a Very potent mixture. Have had great results! Keep it clean.
Edited by Papa_Bear on 09/05/01 12:03 AM.
|
Anonymous
|
Re: How do you use spore prints efficently? [Re: Papa_Bear] 1
#386171 - 09/05/01 08:33 AM (23 years, 7 months ago) |
|
|
See below.Edited by CaptainMaxMushroom on 09/05/01 09:36 AM.
|
Anonymous
|
Re: How do you use spore prints efficently? [Re: Papa_Bear] 1
#386172 - 09/05/01 08:33 AM (23 years, 7 months ago) |
|
|
Here's another syringe making tek:
http://www.sporelab.com/winotek.htm
Good luck!
Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com
Edited by CaptainMaxMushroom on 09/05/01 09:38 AM. Edited by CaptainMaxMushroom on 09/05/01 09:46 AM.
|
bokaj
Stranger
Registered: 09/09/15
Posts: 56
Last seen: 1 year, 7 months
|
 Re: How do you use spore prints efficently? [Re: Anno] 1
#23562657 - 08/21/16 10:53 AM (8 years, 7 months ago) |
|
|
thanks for the tip
|
Kenetic
Nam Sayin
Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land 
Last seen: 6 years, 5 months
|
Re: How do you use spore prints efficently? [Re: bokaj] 1
#23562700 - 08/21/16 11:09 AM (8 years, 7 months ago) |
|
|
--------------------
Todo Cambia
  
  
DMT said: Everyone know's me, they just don't know it yet
|
EyeOnEmitter
Discipulus in campum magnum
Registered: 01/11/18
Posts: 937
Loc: Earth
|
 Re: How do you use spore prints efficently? [Re: Kenetic] 1
#25267959 - 06/14/18 01:40 PM (6 years, 10 months ago) |
|
|
Still a good one though.
Wino tek is cool too.
|
Solipsis
m̶a̶d̶ disappointed scientist
Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands 
Last seen: 1 year, 8 months
|
Re: How do you use spore prints efficently? [Re: EyeOnEmitter] 2
#25268030 - 06/14/18 02:24 PM (6 years, 10 months ago) |
|
|
I'm surprised that nobody mentioned using a loop to streak some spores onto agar? Not sure what kind of efficiency this is actually about but if it is about needing as little as possible that seems to be pretty damn good.
I guess instead this was just about plainly using spores properly... After 15 years i wonder who of those users is still around but hey now it has sort of become about responding to bokaj who is clearly trying to work properly with spores (even if necro bumping probably shouldn't be enabled)
|
EyeOnEmitter
Discipulus in campum magnum
Registered: 01/11/18
Posts: 937
Loc: Earth
|
Re: How do you use spore prints efficently? [Re: Solipsis] 1
#25268063 - 06/14/18 02:43 PM (6 years, 10 months ago) |
|
|
Whoa. What did you just say?
How does anyone deal with spores nowadays anyway?
To a syringe then a jar?
Straight to agar?
I have had only one successful attempt at getting spores to germ on agar, and that was residue left over after sucking the spore mixture into the syringe.
There were 5 potential growths.
Got it narrowed down to three.
Mazapatec var.
Still not even totally sure it is mushroomcelium because it doesn't look like mycelium that i took out of a colonized jar.
Edited by EyeOnEmitter (06/14/18 02:55 PM)
|
Schtarker
Stranger
Registered: 12/29/21
Posts: 120
Last seen: 3 years, 1 month
|
Re: How do you use spore prints efficently? [Re: Anno] 1
#27681238 - 03/03/22 04:07 PM (3 years, 1 month ago) |
|
|
Just so people know, "propanol" is another term for isopropyl alcohol.
|
|
Threaded
Previous
Index
Next
No Unicorns Here—Just Quality Bags That Work 
Isolated Cubensis Liquid Culture For Sale 
Buy Kratom Extract 
Liquid Cultures 
Certified Organic All-In-One Grow Bags 
Bulk Substrate 
Red Vein Kratom
Edit 
Reply 
tekramrepus
Zwieback0
jonocybin
1 2 all )
phrozendata
ShrooomKing