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OfflineRageOholic67
member
Registered: 11/07/02
Posts: 107
Last seen: 14 years, 1 month
My spores are here, but..........
    #1060466 - 11/17/02 07:20 PM (14 years, 4 months ago)

I got my spores tonight. There is something i'm not so sure is good. The little black particles suspended in the water are built up at the tip and in the cap of one of my surringes. Is this normal? Or bad? and is there anything i can do to help it if it isnt normal? Thanks for reading


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: RageOholic67]
    #1060476 - 11/17/02 07:23 PM (14 years, 4 months ago)

the "black particles" are the spores. the rest is just sterile water. make sure you shake it up before you innoculate anything, and you'll be fine.


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OfflineRageOholic67
member
Registered: 11/07/02
Posts: 107
Last seen: 14 years, 1 month
Re: My spores are here, but.......... [Re: MsPacMan]
    #1060478 - 11/17/02 07:24 PM (14 years, 4 months ago)

Cool, but they arent all gone now that there is a build up of them on the needle tip? They are no longer in the steralized liquid, so i shouldnt use them right?


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: RageOholic67]
    #1060486 - 11/17/02 07:28 PM (14 years, 4 months ago)

no its fine. if you are going to trash it, just mail it to me.


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InvisibleTinMan
Stranger

Registered: 10/01/02
Posts: 2,956
Loc: Russia
Re: My spores are here, but.......... [Re: MsPacMan]
    #1060503 - 11/17/02 07:38 PM (14 years, 4 months ago)

As long as you sterilize it with a lighter or alcohol, it'll be fine.


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Offlinesocratesmind
old hand

Registered: 02/22/02
Posts: 1,193
Loc: in your house :)
Last seen: 10 years, 8 months
Re: My spores are here, but.......... [Re: TinMan]
    #1060510 - 11/17/02 07:41 PM (14 years, 4 months ago)

shake after every innoc seems great for my friend. good even growth in 3-5 days under innoc points.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: TinMan]
    #1061510 - 11/18/02 03:32 AM (14 years, 4 months ago)

and let the needle cool after lighting it before innoculating, or you'll kill the spores.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: RageOholic67]
    #1061901 - 11/18/02 10:10 AM (14 years, 4 months ago)

It's best to have an air bubble in the syringe when you shake it as this helps to break up the spore clusters but don't pull unsterilized air into the syringe though as this will infect it! Suck air into the syringe whilst heating the tip of the needle with a flame so as to kill any airborne nasties.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (11/18/02 10:18 AM)


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1061935 - 11/18/02 10:24 AM (14 years, 4 months ago)

why do you even need air in the syringe?


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OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
Last seen: 8 years, 5 months
Re: My spores are here, but.......... [Re: MsPacMan]
    #1061944 - 11/18/02 10:28 AM (14 years, 4 months ago)

What this poster is trying to say is that their syringe arrived with some of the solution having been released, resulting in a spore build-up in the actual syringe cap and the tip of the needle.
I wouldn`t necessarily say it`s a problem but you deffinatelly sound like you lost some of the spore solution.
If it`s too much spore so that your syringe is empty or clear I`d suggest calling/e-mailing the vendor.
Otherwise you should use it but make sure you flame sterilise the needle.


--------------------
Derrayld and out of control


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OfflineTDogg
strangerdanger
Registered: 11/13/02
Posts: 24
Loc: soy norteamericano
Last seen: 14 years, 3 months
Re: My spores are here, but.......... [Re: RageOholic67]
    #1062204 - 11/18/02 12:34 PM (14 years, 4 months ago)

for christ's sake don't pull air into the syringe while holding it over a flame! for obvious reasons.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062207 - 11/18/02 12:38 PM (14 years, 4 months ago)

You dont "need" air in a syringe. It just helps to have air in the syringe when your shaking the thing to break up spore clusters and spores stuck on the side of the syringe which in theory is best for inoculation as theres a more even distribution.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062215 - 11/18/02 12:42 PM (14 years, 4 months ago)

no it doesn't. its not good to assume things, especially when its to give others advice.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: TDogg]
    #1062231 - 11/18/02 12:50 PM (14 years, 4 months ago)

As Morrowind pointed out TDogg theres shit loads of microscopic spores in the sterelized water anyway. I've made a shit load of spore syringes and have never had a dud one from flaming the needle while drawing air in


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062248 - 11/18/02 12:54 PM (14 years, 4 months ago)

No what doesnt MsPacMan ?

Please reiterate.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062257 - 11/18/02 12:56 PM (14 years, 4 months ago)

it doesn't help to have air in a syringe. its asking for contams. all that is needed is to tap the syringe and shake the hell out of it. it'll be fine. as long as every single spore isn't stuck in there, its ok.


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Offlinekb73
enthusiast
Registered: 10/18/01
Posts: 369
Loc: Abilene, TX
Last seen: 6 years, 5 months
Re: My spores are here, but.......... [Re: RageOholic67]
    #1062295 - 11/18/02 01:09 PM (14 years, 4 months ago)

A good way to really shake em up is attach it to a virbrator with some tape or something and turn it on. The high RPMs help loosen stuck spores.


--------------------
WARNING chronicshroom will rip you off! Don't trade with him! I sent him 20 spore syringes and he never sent me anything.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062298 - 11/18/02 01:11 PM (14 years, 4 months ago)

How is it asking for contams when your pulling tha air through a flame ?


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 9 years, 9 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062344 - 11/18/02 01:28 PM (14 years, 4 months ago)

not contams, a flame is very hot, and sucking in hot air will kill the spores.


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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062348 - 11/18/02 01:30 PM (14 years, 4 months ago)

you should NEVER do anything but depress the plunger before the STERILE spore solution is used up. Thats the point, as long as nothing is going into the syringe, then everything comming out will be clean


--------------------
_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


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