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Offlinedets
higher mind andeducation
Registered: 11/12/02
Posts: 91
Last seen: 16 years, 8 months
Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1062364 - 11/18/02 01:37 PM (19 years, 2 months ago)

just take the syringe and shake it up. use it and if your outcome isn't what was expected, then you know where to point the blame at.


--------------------
"patriotism is the virtue of the vicious"


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062489 - 11/18/02 02:08 PM (19 years, 2 months ago)

In response to MsPacMan All I gotta say gal is I got this information from teks featured on the shroomery and I've never made a dud spore syringe yet and I've made loads

Try reading PF-tek for Dummies: http://www.fungifun.org/pf/

Or this one

http://www.shroomery.org/findorgrowthem.php?View=docs&doc=16#Culture




--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (11/18/02 02:35 PM)


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Offlinecookdandbombd
SERENITY NOW!

Registered: 08/31/02
Posts: 148
Loc: Northern Ireland
Last seen: 18 years, 11 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062750 - 11/18/02 03:31 PM (19 years, 2 months ago)

Quote:

In response to MsPacMan All I gotta say gal is I got this information from teks featured on the shroomery and I've never made a dud spore syringe yet and I've made loads





Yeah, it's fine for makinf a spore syringe, but in this case the spore syringe has been ready made, so sucking in air, even "sterile air", is just an unneccesary step and therefore introduces a greater chance of contamination.

In short, give the syringe a shake :laugh:


--------------------
--
Freak out baby, the bee is coming.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 14 years, 7 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1068873 - 11/20/02 06:48 AM (19 years, 2 months ago)

im not trying to call you out or flame you. i was telling him my opinion on what to do, and in my opinion, sucking air into (an already made) syringe is not a good idea, especially burning hot air from a lighter.

and aside from knowing information before you advise someone on it, you should read the post word for word before you try to prove a point. no one was talking about making syringes.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Registered: 06/26/01
Posts: 5,011
Re: My spores are here, but.......... [Re: cookdandbombd]
    #1068884 - 11/20/02 06:59 AM (19 years, 2 months ago)

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Invisibleboxtop703
ThesaurusLinguae Graecae
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Registered: 06/26/01
Posts: 5,011
Re: My spores are here, but.......... [Re: MsPacMan]
    #1068886 - 11/20/02 07:00 AM (19 years, 2 months ago)

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Invisibleboxtop703
ThesaurusLinguae Graecae
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Registered: 06/26/01
Posts: 5,011
Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1068889 - 11/20/02 07:01 AM (19 years, 2 months ago)

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/18/99
Posts: 24,147
Loc: my room
Last seen: 23 days, 15 hours
Re: My spores are here, but.......... [Re: MsPacMan]
    #1068894 - 11/20/02 07:04 AM (19 years, 2 months ago)

>a flame is very hot, and sucking in hot air will kill the spores.

That?s not true.
The hot, sterile air, immediately cools down when it comes in the contact with the water in the spore syringe and the temperature of the syringe rises virtually nothing, sicne the heat capacity of air is so much less than that of water.

As for shaking a completely full syringe, you can shake it for hours, and it still won?t be shaken properly, since the fluid in the syringe can?t move properly.
That?s why it?s better to have a small air bubble in the syringe.

As for "if you suck air in the syringe you will contaminate it"; If you suck in the hot air, you won?t contaminate it, 100% guaranteed.
Even if you suck in "normal" air, the chances are still slim the syringe will contaminate because of this.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Registered: 06/26/01
Posts: 5,011
Re: My spores are here, but.......... [Re: Anno]
    #1068901 - 11/20/02 07:11 AM (19 years, 2 months ago)

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/18/99
Posts: 24,147
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Last seen: 23 days, 15 hours
Re: My spores are here, but.......... [Re: boxtop703]
    #1068907 - 11/20/02 07:19 AM (19 years, 2 months ago)

>Anno, that's not true. There are so many spores in the water it is just rediculous
>to think that simple shaking won't be effective at dispersing the spores. At least
>with my syringes.

Try it. Take 2 identical spore syringes, suck 1 ml of air in the one, and leave the
other completely spore filled. Shake and observe.


>Anno please, your joking right?

Not at all.


>you are saying to go ahead and suck in outside air into the syringe?

Where did I say this?
I said "suck sterile air heated by a flame" into the syringe.

And I said that even if you weren?t sucking in sterile air, the chances you contaminate the syringe are slim. I?m saying so because I tried it on several occasions.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Registered: 06/26/01
Posts: 5,011
Re: My spores are here, but.......... [Re: Anno]
    #1068912 - 11/20/02 07:26 AM (19 years, 2 months ago)

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/18/99
Posts: 24,147
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Last seen: 23 days, 15 hours
Re: My spores are here, but.......... [Re: boxtop703]
    #1068923 - 11/20/02 07:34 AM (19 years, 2 months ago)

>Why would one even try this?

Because the spores woudn?t shake up otherwise, I didn?t know about the sterile air over the flame at that time (a few years ago)

>would most certainly void any sort of sterile work practiced by the maker of the syringe.

The contaminant load of the air we breath is slightly overestimated.
I don?t advise it though neither , if the spores are hard to shake up, I advise to soak a bubble of sterile air from above a flame, it?s easy and guaranteed contaminant free, why take even the smallest risk, if there is a better way of doing it.

Again, try it, and see for yourself.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: Anno]
    #1068973 - 11/20/02 08:24 AM (19 years, 2 months ago)

I totally agree with Anno on this one.

I've made hundreds of spore syringes and never screwed so much as one up pulling air into the syringe through a flame.

Try it kids! It's the latest craze!  :grin: 


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 14 years, 7 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1069013 - 11/20/02 09:01 AM (19 years, 2 months ago)

i dunno. im not sucking any air into my syringes.


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OfflineShtoops
Wonka'sMycologist

Registered: 10/23/02
Posts: 87
Loc: Willy's Oompa Lab
Last seen: 18 years, 7 months
Re: My spores are here, but.......... [Re: MsPacMan]
    #1069202 - 11/20/02 11:02 AM (19 years, 2 months ago)

Everytime I don't suck air into my syringes I get clogs... then I get pissed off that I have a clog and shoot off 5 ml's into one innoculation point...


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OfflineInMyVersion
Stranger

Registered: 11/07/02
Posts: 306
Loc: NE USA
Last seen: 11 years, 11 months
Re: My spores are here, but.......... [Re: Shtoops]
    #1069240 - 11/20/02 11:28 AM (19 years, 2 months ago)

Even though I generally shake my syringes out of habit, I've found that it really doesn't make all that much difference... Sure on occassion you will get one or two inoculation points that will take off faster than the others, but in the end the cakes usually finish in a close tie... Also, most of the syringes from skrowerops usually have a small air bubble in it. If not, rather than disobeying your mother and playing with lighters, try sticking it into the jar, squirt your first inoculation point, find an air pocket in the jar, and suck the 1/4ml of air into the syringe... If you done did things right, it's pretty freakin sterile in there... and if you suck up contams into your syringe from within the jar, my friend you are in for some disappointment... this to me is the easiest, least time consuming way to do it, and I don't have to worry about burning my fingers off with those dangerous lighters!


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OfflineSka_mad_hatter
CT Trips

Registered: 10/06/02
Posts: 39
Loc: Cheshire Connecticut
Last seen: 19 years, 1 month
Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1069321 - 11/20/02 12:13 PM (19 years, 2 months ago)

i have only inccubated jars twice in my life but both times i have put the needle to flame to suck in a tiny air bubble.. There is no way to shake that syringe poperly without one. Both times most of my jars have colonized properly.


--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always
worked for me.
Hunter S. Thompson


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
Posts: 240
Last seen: 18 years, 4 months
Re: My spores are here, but.......... [Re: Ska_mad_hatter]
    #1069404 - 11/20/02 12:41 PM (19 years, 2 months ago)

I have sucked air into my syringe. Guess what happened. Every Jar I had except one contamed.


--------------------
"Man was born free and is every where in chains."


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: lvleph]
    #1069483 - 11/20/02 01:09 PM (19 years, 2 months ago)

In reply to:

I have sucked air into my syringe. Guess what happened. Every Jar I had except one contamed.




Well obviously your jars were contaminated then !?


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
Posts: 240
Last seen: 18 years, 4 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1069495 - 11/20/02 01:12 PM (19 years, 2 months ago)

yeah and where ever I am there I am.


--------------------
"Man was born free and is every where in chains."


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