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OfflineRageOholic67
member
Registered: 11/07/02
Posts: 107
Last seen: 18 years, 11 months
My spores are here, but..........
    #1060466 - 11/17/02 07:20 PM (19 years, 2 months ago)

I got my spores tonight. There is something i'm not so sure is good. The little black particles suspended in the water are built up at the tip and in the cap of one of my surringes. Is this normal? Or bad? and is there anything i can do to help it if it isnt normal? Thanks for reading


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OfflineMsPacMan
Stranger

Registered: 10/05/02
Posts: 1,054
Loc: Florida, USA
Last seen: 14 years, 7 months
Re: My spores are here, but.......... [Re: RageOholic67]
    #1060476 - 11/17/02 07:23 PM (19 years, 2 months ago)

the "black particles" are the spores. the rest is just sterile water. make sure you shake it up before you innoculate anything, and you'll be fine.


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OfflineRageOholic67
member
Registered: 11/07/02
Posts: 107
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Re: My spores are here, but.......... [Re: MsPacMan]
    #1060478 - 11/17/02 07:24 PM (19 years, 2 months ago)

Cool, but they arent all gone now that there is a build up of them on the needle tip? They are no longer in the steralized liquid, so i shouldnt use them right?


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OfflineMsPacMan
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Registered: 10/05/02
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Re: My spores are here, but.......... [Re: RageOholic67]
    #1060486 - 11/17/02 07:28 PM (19 years, 2 months ago)

no its fine. if you are going to trash it, just mail it to me.


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InvisibleTinMan
Stranger

Registered: 10/01/02
Posts: 2,956
Loc: Russia
Re: My spores are here, but.......... [Re: MsPacMan]
    #1060503 - 11/17/02 07:38 PM (19 years, 2 months ago)

As long as you sterilize it with a lighter or alcohol, it'll be fine.


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Offlinesocratesmind
old hand

Registered: 02/22/02
Posts: 1,193
Loc: in your house :)
Last seen: 15 years, 6 months
Re: My spores are here, but.......... [Re: TinMan]
    #1060510 - 11/17/02 07:41 PM (19 years, 2 months ago)

shake after every innoc seems great for my friend. good even growth in 3-5 days under innoc points.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: TinMan]
    #1061510 - 11/18/02 03:32 AM (19 years, 2 months ago)

and let the needle cool after lighting it before innoculating, or you'll kill the spores.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: RageOholic67]
    #1061901 - 11/18/02 10:10 AM (19 years, 2 months ago)

It's best to have an air bubble in the syringe when you shake it as this helps to break up the spore clusters but don't pull unsterilized air into the syringe though as this will infect it! Suck air into the syringe whilst heating the tip of the needle with a flame so as to kill any airborne nasties.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (11/18/02 10:18 AM)


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1061935 - 11/18/02 10:24 AM (19 years, 2 months ago)

why do you even need air in the syringe?


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OfflineDERRAYLD
Constructus
Male

Registered: 05/13/02
Posts: 3,800
Loc: South Africa
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Re: My spores are here, but.......... [Re: MsPacMan]
    #1061944 - 11/18/02 10:28 AM (19 years, 2 months ago)

What this poster is trying to say is that their syringe arrived with some of the solution having been released, resulting in a spore build-up in the actual syringe cap and the tip of the needle.
I wouldn`t necessarily say it`s a problem but you deffinatelly sound like you lost some of the spore solution.
If it`s too much spore so that your syringe is empty or clear I`d suggest calling/e-mailing the vendor.
Otherwise you should use it but make sure you flame sterilise the needle.


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OfflineTDogg
strangerdanger
Registered: 11/13/02
Posts: 24
Loc: soy norteamericano
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Re: My spores are here, but.......... [Re: RageOholic67]
    #1062204 - 11/18/02 12:34 PM (19 years, 2 months ago)

for christ's sake don't pull air into the syringe while holding it over a flame! for obvious reasons.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062207 - 11/18/02 12:38 PM (19 years, 2 months ago)

You dont "need" air in a syringe. It just helps to have air in the syringe when your shaking the thing to break up spore clusters and spores stuck on the side of the syringe which in theory is best for inoculation as theres a more even distribution.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062215 - 11/18/02 12:42 PM (19 years, 2 months ago)

no it doesn't. its not good to assume things, especially when its to give others advice.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: TDogg]
    #1062231 - 11/18/02 12:50 PM (19 years, 2 months ago)

As Morrowind pointed out TDogg theres shit loads of microscopic spores in the sterelized water anyway. I've made a shit load of spore syringes and have never had a dud one from flaming the needle while drawing air in


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062248 - 11/18/02 12:54 PM (19 years, 2 months ago)

No what doesnt MsPacMan ?

Please reiterate.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062257 - 11/18/02 12:56 PM (19 years, 2 months ago)

it doesn't help to have air in a syringe. its asking for contams. all that is needed is to tap the syringe and shake the hell out of it. it'll be fine. as long as every single spore isn't stuck in there, its ok.


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Offlinekb73
enthusiast
Registered: 10/18/01
Posts: 369
Loc: Abilene, TX
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Re: My spores are here, but.......... [Re: RageOholic67]
    #1062295 - 11/18/02 01:09 PM (19 years, 2 months ago)

A good way to really shake em up is attach it to a virbrator with some tape or something and turn it on. The high RPMs help loosen stuck spores.


--------------------
WARNING chronicshroom will rip you off! Don't trade with him! I sent him 20 spore syringes and he never sent me anything.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062298 - 11/18/02 01:11 PM (19 years, 2 months ago)

How is it asking for contams when your pulling tha air through a flame ?


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062344 - 11/18/02 01:28 PM (19 years, 2 months ago)

not contams, a flame is very hot, and sucking in hot air will kill the spores.


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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062348 - 11/18/02 01:30 PM (19 years, 2 months ago)

you should NEVER do anything but depress the plunger before the STERILE spore solution is used up. Thats the point, as long as nothing is going into the syringe, then everything comming out will be clean


--------------------
_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


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Offlinedets
higher mind andeducation
Registered: 11/12/02
Posts: 91
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Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1062364 - 11/18/02 01:37 PM (19 years, 2 months ago)

just take the syringe and shake it up. use it and if your outcome isn't what was expected, then you know where to point the blame at.


--------------------
"patriotism is the virtue of the vicious"


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: MsPacMan]
    #1062489 - 11/18/02 02:08 PM (19 years, 2 months ago)

In response to MsPacMan All I gotta say gal is I got this information from teks featured on the shroomery and I've never made a dud spore syringe yet and I've made loads

Try reading PF-tek for Dummies: http://www.fungifun.org/pf/

Or this one

http://www.shroomery.org/findorgrowthem.php?View=docs&doc=16#Culture




--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (11/18/02 02:35 PM)


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Offlinecookdandbombd
SERENITY NOW!

Registered: 08/31/02
Posts: 148
Loc: Northern Ireland
Last seen: 18 years, 11 months
Re: My spores are here, but.......... [Re: Bi0TeK]
    #1062750 - 11/18/02 03:31 PM (19 years, 2 months ago)

Quote:

In response to MsPacMan All I gotta say gal is I got this information from teks featured on the shroomery and I've never made a dud spore syringe yet and I've made loads





Yeah, it's fine for makinf a spore syringe, but in this case the spore syringe has been ready made, so sucking in air, even "sterile air", is just an unneccesary step and therefore introduces a greater chance of contamination.

In short, give the syringe a shake :laugh:


--------------------
--
Freak out baby, the bee is coming.


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OfflineMsPacMan
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1068873 - 11/20/02 06:48 AM (19 years, 2 months ago)

im not trying to call you out or flame you. i was telling him my opinion on what to do, and in my opinion, sucking air into (an already made) syringe is not a good idea, especially burning hot air from a lighter.

and aside from knowing information before you advise someone on it, you should read the post word for word before you try to prove a point. no one was talking about making syringes.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Re: My spores are here, but.......... [Re: cookdandbombd]
    #1068884 - 11/20/02 06:59 AM (19 years, 2 months ago)

Post deleted by administrator.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Re: My spores are here, but.......... [Re: MsPacMan]
    #1068886 - 11/20/02 07:00 AM (19 years, 2 months ago)

Post deleted by administrator.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1068889 - 11/20/02 07:01 AM (19 years, 2 months ago)

Post deleted by administrator.


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OfflineAnnoA
Experimenter
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Re: My spores are here, but.......... [Re: MsPacMan]
    #1068894 - 11/20/02 07:04 AM (19 years, 2 months ago)

>a flame is very hot, and sucking in hot air will kill the spores.

That?s not true.
The hot, sterile air, immediately cools down when it comes in the contact with the water in the spore syringe and the temperature of the syringe rises virtually nothing, sicne the heat capacity of air is so much less than that of water.

As for shaking a completely full syringe, you can shake it for hours, and it still won?t be shaken properly, since the fluid in the syringe can?t move properly.
That?s why it?s better to have a small air bubble in the syringe.

As for "if you suck air in the syringe you will contaminate it"; If you suck in the hot air, you won?t contaminate it, 100% guaranteed.
Even if you suck in "normal" air, the chances are still slim the syringe will contaminate because of this.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Re: My spores are here, but.......... [Re: Anno]
    #1068901 - 11/20/02 07:11 AM (19 years, 2 months ago)

Post deleted by administrator.


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OfflineAnnoA
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Re: My spores are here, but.......... [Re: boxtop703]
    #1068907 - 11/20/02 07:19 AM (19 years, 2 months ago)

>Anno, that's not true. There are so many spores in the water it is just rediculous
>to think that simple shaking won't be effective at dispersing the spores. At least
>with my syringes.

Try it. Take 2 identical spore syringes, suck 1 ml of air in the one, and leave the
other completely spore filled. Shake and observe.


>Anno please, your joking right?

Not at all.


>you are saying to go ahead and suck in outside air into the syringe?

Where did I say this?
I said "suck sterile air heated by a flame" into the syringe.

And I said that even if you weren?t sucking in sterile air, the chances you contaminate the syringe are slim. I?m saying so because I tried it on several occasions.


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Invisibleboxtop703
ThesaurusLinguae Graecae
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Re: My spores are here, but.......... [Re: Anno]
    #1068912 - 11/20/02 07:26 AM (19 years, 2 months ago)

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OfflineAnnoA
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Re: My spores are here, but.......... [Re: boxtop703]
    #1068923 - 11/20/02 07:34 AM (19 years, 2 months ago)

>Why would one even try this?

Because the spores woudn?t shake up otherwise, I didn?t know about the sterile air over the flame at that time (a few years ago)

>would most certainly void any sort of sterile work practiced by the maker of the syringe.

The contaminant load of the air we breath is slightly overestimated.
I don?t advise it though neither , if the spores are hard to shake up, I advise to soak a bubble of sterile air from above a flame, it?s easy and guaranteed contaminant free, why take even the smallest risk, if there is a better way of doing it.

Again, try it, and see for yourself.


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: Anno]
    #1068973 - 11/20/02 08:24 AM (19 years, 2 months ago)

I totally agree with Anno on this one.

I've made hundreds of spore syringes and never screwed so much as one up pulling air into the syringe through a flame.

Try it kids! It's the latest craze!  :grin: 


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMsPacMan
Stranger

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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1069013 - 11/20/02 09:01 AM (19 years, 2 months ago)

i dunno. im not sucking any air into my syringes.


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OfflineShtoops
Wonka'sMycologist

Registered: 10/23/02
Posts: 87
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Re: My spores are here, but.......... [Re: MsPacMan]
    #1069202 - 11/20/02 11:02 AM (19 years, 2 months ago)

Everytime I don't suck air into my syringes I get clogs... then I get pissed off that I have a clog and shoot off 5 ml's into one innoculation point...


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OfflineInMyVersion
Stranger

Registered: 11/07/02
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Re: My spores are here, but.......... [Re: Shtoops]
    #1069240 - 11/20/02 11:28 AM (19 years, 2 months ago)

Even though I generally shake my syringes out of habit, I've found that it really doesn't make all that much difference... Sure on occassion you will get one or two inoculation points that will take off faster than the others, but in the end the cakes usually finish in a close tie... Also, most of the syringes from skrowerops usually have a small air bubble in it. If not, rather than disobeying your mother and playing with lighters, try sticking it into the jar, squirt your first inoculation point, find an air pocket in the jar, and suck the 1/4ml of air into the syringe... If you done did things right, it's pretty freakin sterile in there... and if you suck up contams into your syringe from within the jar, my friend you are in for some disappointment... this to me is the easiest, least time consuming way to do it, and I don't have to worry about burning my fingers off with those dangerous lighters!


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OfflineSka_mad_hatter
CT Trips

Registered: 10/06/02
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Loc: Cheshire Connecticut
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Re: My spores are here, but.......... [Re: Cow Shit Collector]
    #1069321 - 11/20/02 12:13 PM (19 years, 2 months ago)

i have only inccubated jars twice in my life but both times i have put the needle to flame to suck in a tiny air bubble.. There is no way to shake that syringe poperly without one. Both times most of my jars have colonized properly.


--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always
worked for me.
Hunter S. Thompson


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
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Re: My spores are here, but.......... [Re: Ska_mad_hatter]
    #1069404 - 11/20/02 12:41 PM (19 years, 2 months ago)

I have sucked air into my syringe. Guess what happened. Every Jar I had except one contamed.


--------------------
"Man was born free and is every where in chains."


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: My spores are here, but.......... [Re: lvleph]
    #1069483 - 11/20/02 01:09 PM (19 years, 2 months ago)

In reply to:

I have sucked air into my syringe. Guess what happened. Every Jar I had except one contamed.




Well obviously your jars were contaminated then !?


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1069495 - 11/20/02 01:12 PM (19 years, 2 months ago)

yeah and where ever I am there I am.


--------------------
"Man was born free and is every where in chains."


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OfflineTheHobbit
Pot Head Pixie

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Re: My spores are here, but.......... [Re: Anno]
    #1069518 - 11/20/02 01:18 PM (19 years, 2 months ago)

"if the spores are hard to shake up, I advise to soak a bubble of sterile air from above a flame"

  Glad I ran across this, it does seem kinda hard to get things shaken up well, gonna give i a shot next time I innoc :smile:


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
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Re: My spores are here, but.......... [Re: Bi0TeK]
    #1069520 - 11/20/02 01:18 PM (19 years, 2 months ago)

'IT" has yet to use a syringe to inoc with . But when the time comes he will listen to boxtop . I agree why risk contams after all the hard work of vendor to prevent foriegn nasties from being in the syringe . Plus how could you excpect them to honor any sort of guarantee , when you take it upon yourself to "hope" you did not suck in dirty air . I like the idea of using a vibrator more than the sucking in of air that I have no idea if was sterile or not . Flicking the side of the syringe should break up clumps , thus making shaking a bit more effective .
Now if we all had use of a true "clean room "; than sucking in some air would be ideal . Till then ....shake shake shake your spores ! :wink: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinelvleph
mathematicalidiot

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Re: My spores are here, but.......... [Re: tripndicular]
    #1069537 - 11/20/02 01:21 PM (19 years, 2 months ago)

If you have a washing machine stick it on top of that when it is on the spin cycle. That should do it.


--------------------
"Man was born free and is every where in chains."


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OfflineStonedhenge
searcher

Registered: 10/05/02
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Re: My spores are here, but.......... [Re: Anno]
    #1069693 - 11/20/02 01:53 PM (19 years, 2 months ago)

A friend says you can pull a bubble thru a piece of paper towel with alcohol on it,
and it works great


--------------------
good for nothing's good enough for me!


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OfflineAnnoA
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Re: My spores are here, but.......... [Re: Stonedhenge]
    #1069827 - 11/20/02 02:20 PM (19 years, 2 months ago)

>A friend says you can pull a bubble thru a piece of paper towel with alcohol on it,

Sounds reasonable.


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OfflineMekyle7
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Re: My spores are here, but.......... [Re: Anno]
    #4200864 - 05/20/05 07:51 PM (16 years, 8 months ago)

I use a vibrator. While it is still in the vacuum seal I put a vibrator under it and come back in a minute. Yay.


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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: My spores are here, but.......... [Re: Mekyle7]
    #4201287 - 05/20/05 10:01 PM (16 years, 8 months ago)

way to go...bringing an old post back from the dead!~

use one of these.


shake spores during the day!~

shake the old lady at night!~

lolzz


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSammy
Between the fold

Registered: 09/03/02
Posts: 1,031
Loc: you'll shoot yer eye out ...
Last seen: 14 years, 1 month
Re: My spores are here, but.......... [Re: Roadkill]
    #4201303 - 05/20/05 10:07 PM (16 years, 8 months ago)

Quote:

Roadkill said:
way to go...bringing an old post back from the dead!~

use one of these.


shake spores during the day!~

shake the old lady at night!~

lolzz




:whoa: :whoa: :whoa: :whoa: :whoa: :whoa: :whoa: :whoa: :whoa: :whoa:

That's some serious machinery! I couldn't imagine the woman that might be tempted to try that being my type.  I dunno.. just a thought.  :tongue:

Sam


--------------------

I believe in the Golden Rule ? The Man with the Gold . . . Rules.
- Mr. T


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