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InvisibleJ3illy
Trainee

Registered: 10/18/08
Posts: 3,344
Re: LC Cloning vs Isolating [Re: arp180]
    #9375370 - 12/05/08 08:16 PM (15 years, 3 months ago)

Quote:

arp180 said:
"From reading about both"

I am glad to see you started your post with this.




That's the tone that my posts pretty much always have.  I don't claim to have done much yet, but I sure have read a hell of a lot - I mean, look at my title I've had since I joined lol.

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InvisibleJ3illy
Trainee

Registered: 10/18/08
Posts: 3,344
Re: LC Cloning vs Isolating [Re: nw_shroomy]
    #9375380 - 12/05/08 08:19 PM (15 years, 3 months ago)

Quote:

nw_shroomy said:
I'm sure this can be answered with search but since its being discussed.
How long after transferring to agar does it take to grow before its time to transfer to the next agar?




I think it would take like a week to have the whole plate fill up.  But you'd be seeing growth in a few days

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OfflineLightShedder
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Registered: 08/30/05
Posts: 3,026
Loc: AustinDenverLA
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Re: LC Cloning vs Isolating [Re: nw_shroomy]
    #9375381 - 12/05/08 08:19 PM (15 years, 3 months ago)

anywhere from 3-7 days depending on that particular substrain and temperature etc. Usually the chunk will recover after 2-3 days and grow from there. I like to do the isolations as soon as you see any growth coming from the chunk. This is to minimize contamination and also to prevent the further mating of new strains.


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Invisiblenw_shroomy
NoN-stranger
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Registered: 01/02/06
Posts: 1,332
Re: LC Cloning vs Isolating [Re: LightShedder]
    #9375514 - 12/05/08 08:40 PM (15 years, 3 months ago)

Quote:

LightShedder said:
anywhere from 3-7 days depending on that particular substrain and temperature etc. Usually the chunk will recover after 2-3 days and grow from there. I like to do the isolations as soon as you see any growth coming from the chunk. This is to minimize contamination and also to prevent the further mating of new strains.


How many times Does it take to get to the one you want?


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Spawn Ratio Calculator
http://www.shroomery.org/forums/showflat.php/Number/7803673#7803673
I only grow edibles.Any info I give ONLY applies to gourmet mushrooms.

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OfflineLightShedder
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Registered: 08/30/05
Posts: 3,026
Loc: AustinDenverLA
Last seen: 4 years, 9 months
Re: LC Cloning vs Isolating [Re: nw_shroomy]
    #9375567 - 12/05/08 08:45 PM (15 years, 3 months ago)

depends. take the smallest chunks possible and you will minimize the amount of transfers necessary but even then, it is dependent on the amount of substrains originally on the plate. It shouldn't take more then 3 or 4 transfers.


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