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OfflineSHROOMpimp
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Is there a Poor Man's way to ISOLATE?
    #4040213 - 04/11/05 10:24 AM (12 years, 1 month ago)

just curious how people isolate they're mushies. i'm unfamiliar w/ how to do agar or clones... i would like to start working w/ both. could someone give the the basic run down of how to do these, and also what the pourpose of each step, so i can truely understand what is going on while the process is underway. well, i just figured i'd ask because there's several of you guys that know what your talking about, and i trust ya'll opinions greatly. hopefully this will help me gain an understanding of what i'm wanting to do.

THANX A MILLION!!!
SHROOMpimp


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:safesex: Just My $0.02... :safesex:


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Offlinepsilocyben
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040231 - 04/11/05 10:34 AM (12 years, 1 month ago)

4% light corn syrup (karo)
to 96% water (clean)

you can "core" it by bi-secting the stem, then running the syringe lengthwise down th stem, while the tissue gathers in the needle.
make sure you have a little bit of clean water in there too.
then, squirt it in your liquid medium.
all of this is to be performed in as sterile conditions as you can provide.


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OfflineSHROOMpimp
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Re: Is there a Poor Man's way to ISOLATE? [Re: psilocyben]
    #4040238 - 04/11/05 10:39 AM (12 years, 1 month ago)

and that's all there is to isolating? i just pick the exact mushie that i want, then split and gather a little tissue in the needle? how much tissue? man that sounds sooooo easy. thanx a million man!!!

SHROOMpimp


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OfflineSHROOMpimp
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040245 - 04/11/05 10:42 AM (12 years, 1 month ago)

oh, i forgot, is there an age that is best to isolate at? maturity? during growth? sorry to ask so many questions, but i'm about to do it right now and it just sounds too easy to be true! lol thanx

SHROOMpimp


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Offlinethenewguy05
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040275 - 04/11/05 10:54 AM (12 years, 1 month ago)

in my experience no age is really a factor just the freshness of the live culture you are trying to take.As a side note younger is better meaning after the veil breaks but before it starts to decompose. Besides using a syringe i use a brand new razor blade and just chop it in half and cut a little triangular shaped wedge out of the center.not that there is anything wrong with coring it is recommended, I just don't have the time to core it like a banana.


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Offlineblackout
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Re: Is there a Poor Man's way to ISOLATE? [Re: thenewguy05]
    #4040284 - 04/11/05 10:58 AM (12 years, 1 month ago)

It is that easy, I'm surprised more don't do it. Many in the EU can buy fresh mushrooms for the same price as a syringe/print so you can start with a pure striain easily and eat the rest!


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Offlinethenewguy05
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Re: Is there a Poor Man's way to ISOLATE? [Re: blackout]
    #4040298 - 04/11/05 11:05 AM (12 years, 1 month ago)

i was brought onto the idea when i saw the price for a live white button culture. $38 i said yeah right and cloned some from the store for a fraction of the price.


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OfflineSHROOMpimp
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Re: Is there a Poor Man's way to ISOLATE? [Re: blackout]
    #4040300 - 04/11/05 11:06 AM (12 years, 1 month ago)

wow, ok.... this is the type of stuff i'm looking for! does the veil have to break? i usually harvest mine just before the veil breaks, unless i plan on printing. after you isolate (via coreing or cutting) do you just treat the karo water mix w/ the chunk of shroom like it was a LC? and say you have fruited a full flush of an isolate, if you print one is that an isolate print or is that multi-spore or whatever you want to call it?

thanx,
SHROOMpimp


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OfflinekronnyQ
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040309 - 04/11/05 11:09 AM (12 years, 1 month ago)

Is there a poor man's way to isolate a culture from spores?

I have a syringe of PF that seems to be contaminated.


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Offlinepsilocyben
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Re: Is there a Poor Man's way to ISOLATE? [Re: kronnyQ]
    #4040343 - 04/11/05 11:21 AM (12 years, 1 month ago)

Quote:

kronnyQ said:
Is there a poor man's way to isolate a culture from spores?

I have a syringe of PF that seems to be contaminated.




you can't clone many spores; they would all germinate and you would have diversity.
if it is contaminated with bacteria, you can clean it on agar using antibiotics, but hardly woth your trouble.

and SP-
you're going to want to peroxidate that liquid medium too.
this is the advantage to doing tissue cultures:
spores will not grow on peroxidated agar/karo. (this includes contaminant's too)
however mycelia will spread from the tissue onto the peoxidated liquid medium.

haven't done it yet, and notes are somehwere else. (As far as your next question on ratios is concerned)
all of this, (with maybe the exception of the coring technique {olg's])
can be found in the cultivation subforums under the "agar" sub-category.

go check it out


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OfflineSHROOMpimp
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Re: Is there a Poor Man's way to ISOLATE? [Re: psilocyben]
    #4040353 - 04/11/05 11:24 AM (12 years, 1 month ago)

on my way there now! thanx a million! hope to show some pix of my first isolate soon! hehe

SHROOMpimp


--------------------
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Offlinethenewguy05
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040378 - 04/11/05 11:31 AM (12 years, 1 month ago)

Quote:

SHROOMpimp said:
does the veil have to break? i usually harvest mine just before the veil breaks


no the veil doen't have to break. just as long as it is fresh.


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OfflineSHROOMpimp
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Re: Is there a Poor Man's way to ISOLATE? [Re: thenewguy05]
    #4040405 - 04/11/05 11:40 AM (12 years, 1 month ago)

cool, thanx, i got a bad ass that i'm wanting to isolate! and when i get it growing is it possible to stall in the fridge? or just use it up?


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Offlinethenewguy05
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040421 - 04/11/05 11:49 AM (12 years, 1 month ago)

well I'm not exactly sure but i can tell you that mycologists leave agar media in the fridge for years to preserve a pure culture. when mycelium temp is reduced to 34-36 degrees Fahrenheit it pretty much stops growth until reheated to a habitable temperature.


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Offlineblackout
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Re: Is there a Poor Man's way to ISOLATE? [Re: thenewguy05]
    #4040522 - 04/11/05 12:23 PM (12 years, 1 month ago)

I'm surprised there are not more strains available since it is such an easy way to start growing from a wild shroom. Rather than having do deal with contaminated wild spore wild prints, just cut open and you have it.

Cloning sclerotia is easy too and since it is from sclerotia you can be sure the isolated strain will be sclerotia producing. Again EU members could go to a shop selling sclerotia and offer to buy just one stone rather than pay for the expensive sclerotia producing prints/syringes.

Maybe the fsre could buy a box of stones and send them out like prints.


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InvisibleRoadkill
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Re: Is there a Poor Man's way to ISOLATE? [Re: SHROOMpimp]
    #4040548 - 04/11/05 12:31 PM (12 years, 1 month ago)

here is a poor mans way to start working with agar.

use wide mouth 1/2 pint jars instead of petri dishes.

pick up some agar agar at a chinese market...or a health food store.

pick up some Nutritional Yeast at a health food store...or a grocery store that has bulk foods.

pick up a box of instant potato's...Potato Buds Brand

buy some honey....or get some of the Mc Donalds honey. :smile:

---

The recipe that I use:

-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast

---

I like to use the PDYA and then switch back and forth with MEA.

---

Other Agar recipies!~


You will really enjoy working with agar once you get started.

You can find good deals on petri dishes on Ebay...you just have to keep checking.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinescatmanrav
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Re: Is there a Poor Man's way to ISOLATE? [Re: Roadkill]
    #4040559 - 04/11/05 12:33 PM (12 years, 1 month ago)

You can also just use a peice of moist carboard in a 1/2 pint or petri dish..though it will be more difficult to isolate on this because its harder to cut. Works for generating spawn though and cloning and such. The cardboard tek can be found around here somewhere. I'd just do some PDA though like that ^^^^^


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleNeedMoreSleep
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Re: Is there a Poor Man's way to ISOLATE? [Re: scatmanrav]
    #4040627 - 04/11/05 12:44 PM (12 years, 1 month ago)

Good thread, I've been wanting to jumpt to agar as well...  PDA? Potato Dextrose Agar??? PDYA??? :confused:

edit: potato dextrose yeast agar???


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


Edited by NeedMoreSleep (04/11/05 12:45 PM)


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InvisibleRoadkill
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Re: Is there a Poor Man's way to ISOLATE? [Re: blackout]
    #4040628 - 04/11/05 12:44 PM (12 years, 1 month ago)

Quote:

blackout said:

I'm surprised there are not more strains available since it is such an easy way to start growing from a wild shroom. Rather than having do deal with contaminated wild spore wild prints, just cut open and you have it.





There are alot of new strains being worked on right now.
I know of several people starting some new strains right now. :smile:
I have 3 new strains that I'm working on right now.

The problem is that most of these new strains are found all over the world...and can't be sent fresh...for several reasons.
So they have to be started from Wild Spore Prints.
The people that find these new strains of Cubensis...usually aren't people that are working with the new strains.
Example : John Allen...he sends his new findings (strains) to other people like workman, Ralphster and myself...to work on.

If you are very good with working on agar...working with Wild spore prints isn't a problem.

TC


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinescatmanrav
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Re: Is there a Poor Man's way to ISOLATE? [Re: Roadkill]
    #4040640 - 04/11/05 12:46 PM (12 years, 1 month ago)

>PDYA

Potato Dextrose YEAST Agar.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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