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Invisibleaaron
member

Registered: 10/18/99
Posts: 89
Experiments in Storeing Cultures Under Mineral Oil
    #91524 - 01/03/00 11:55 PM (16 years, 11 months ago)

They were stored with and without mineral oil, for six years.
Mineral oil = produced sporeless fruitbodies (sterile).
Without = were extremely viable and produced fertile offspring.
I would suggest water layers as an alternative or maybe (10% glycerin 90% water) for the dung dwellers.

AAron



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Anonymous

Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91525 - 01/04/00 02:04 AM (16 years, 11 months ago)

So you stored the mycelium under mineral water for 6 years and when the mycelium fruited it produced mushrooms without spores? I've heard that ingesting spores can cause upset stomach and things of that nature, could this possibly be a good thing?

------------------
noisecode
noisecode@fnmail.com



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Invisibleaaron
member

Registered: 10/18/99
Posts: 89
Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91527 - 01/08/00 01:57 AM (16 years, 11 months ago)

!!@@##

[This message has been edited by aaron (edited January 08, 2000).]



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Invisibleaaron
member

Registered: 10/18/99
Posts: 89
Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91528 - 01/08/00 02:01 AM (16 years, 11 months ago)

Noisecode
Storing under mineral oil shows miajor signs of degeneration. Some of the effects were sporeless,irregular growth,and the inability to orient themselves correctly.This is a bad thing.

Moe Howard
Don,t store cultures under mineral oil.
The control group show les degradation and they were stored with NOTHING they sat in a frig for six years.
It only benifits those who have a cultures which they love and want to keep for long period of time.

HERE A FEW METHODS
Mineral oil storage; for mycelium.
Water storage; plug method,or water layer; for spores or mycelium.
Soil storage or compost; for mycelium.
Sillica gel storagr; only for spores.
Variations of the above using glycerin instead of water.
Freezing under giycerin; for spores and mycelium.
These are the most commonly used.

------------------
AARON

--------------------------------
I don't know what happened.
I must have walked into a spore.



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Anonymous

Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91529 - 01/08/00 05:41 AM (16 years, 11 months ago)

Parafin.

------------------

What little I know....



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Anonymous

Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91530 - 01/08/00 06:17 AM (16 years, 11 months ago)

Hey Doc . . . parafin does work ! I don't do this myself, however, back in the dark ages ('60s), we DID use parafin for shroom/spore, long-term storage!

I must also thank Prof. Wilkenssen (I always suspected he was "sporing" more than shitakes)

------------------
<<<~~~~~~~~~~>>>
. . . and all the people gathered in their secret place, deep in the forest, to share the wondrous fruits of the roots . . .

. . . and they knew the glory of being.



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Anonymous

Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #91531 - 01/08/00 01:41 PM (16 years, 11 months ago)

this of course means that dr stamets book is full of shit!

---the mineral oil nonsense is from HIS BOOK!

figures...



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OfflineNickthetrip
Super Saiyan

Registered: 06/11/02
Posts: 65
Loc: Ever tryed this position ...
Last seen: 14 years, 9 days
Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
    #673176 - 06/11/02 01:44 PM (14 years, 6 months ago)

I tryed to quick freeze mycelium using a CO2 quick release gun while the mycelium was in a container and sealed it and threw into a freezer
I hope that it wouldn't damage the tissue of the mycelium thats why I froze it in a 5second period with the gun but I prayed...and it worked somehow I dont know how but after thawing it was able to produce fruit of couse I have no control on this experiment (as in years) but hey if it worked maybe it'll last years to come as long as it is in a sorta air tite vaccum container... If you have any questions about this or comments please reply!!!


--------------------
"Crawling in my skin, these wounds they will not heal, fear is how I fall, confusing, confusing what is real!."-Linkin Park


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OfflineOrchidman
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Registered: 04/01/02
Posts: 205
Loc: Toronto
Last seen: 14 years, 1 month
Re: Experiments in Storeing Cultures Under Mineral Oil [Re: Nickthetrip]
    #675865 - 06/13/02 01:06 AM (14 years, 5 months ago)

I believe that once the mycellium is frozen with the CO2 gun it can last for a very long time frozen. The damage in freezing occurs when the water in the mycellium reaches 32f and at this temp the water expands and bursts the cells. I am curious as to why the cells did not burst as the mycellium thawed and was exposed to a much longer period of 32f than the quick freeze. I think the praying must have done it.
The damage in the freezer is from drying so an airtight container is a must.
Maybe you will develope a strain of freeze tolerant Cubensis or Pan tropicalis to grow in northern gardens--just wishing.


--------------------
I've been having psilly dreams lately


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