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 aaron
Pioneering spirit
Registered: 10/17/99
Posts: 100
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 Experiments in Storeing Cultures Under Mineral Oil 
#91524 - 01/03/00 09:55 PM (24 years, 8 months ago) |
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They were stored with and without mineral oil, for six years.
Mineral oil = produced sporeless fruitbodies (sterile).
Without = were extremely viable and produced fertile offspring.
I would suggest water layers as an alternative or maybe (10% glycerin 90% water) for the dung dwellers. AAron
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Anonymous
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91525 - 01/04/00 12:04 AM (24 years, 8 months ago) |
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So you stored the mycelium under mineral water for 6 years and when the mycelium fruited it produced mushrooms without spores? I've heard that ingesting spores can cause upset stomach and things of that nature, could this possibly be a good thing?
------------------
noisecode
noisecode@fnmail.com
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aaron
Pioneering spirit
Registered: 10/17/99
Posts: 100
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91527 - 01/07/00 11:57 PM (24 years, 8 months ago) |
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!!@@##[This message has been edited by aaron (edited January 08, 2000).]
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aaron
Pioneering spirit
Registered: 10/17/99
Posts: 100
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91528 - 01/08/00 12:01 AM (24 years, 8 months ago) |
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Noisecode
Storing under mineral oil shows miajor signs of degeneration. Some of the effects were sporeless,irregular growth,and the inability to orient themselves correctly.This is a bad thing.Moe Howard
Don,t store cultures under mineral oil.
The control group show les degradation and they were stored with NOTHING they sat in a frig for six years.
It only benifits those who have a cultures which they love and want to keep for long period of time. HERE A FEW METHODS
Mineral oil storage; for mycelium.
Water storage; plug method,or water layer; for spores or mycelium.
Soil storage or compost; for mycelium.
Sillica gel storagr; only for spores.
Variations of the above using glycerin instead of water.
Freezing under giycerin; for spores and mycelium.
These are the most commonly used. ------------------
AARON --------------------------------
I don't know what happened.
I must have walked into a spore.
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Anonymous
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91529 - 01/08/00 03:41 AM (24 years, 8 months ago) |
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Parafin.------------------
What little I know....
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Anonymous
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91530 - 01/08/00 04:17 AM (24 years, 8 months ago) |
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Hey Doc . . . parafin does work ! I don't do this myself, however, back in the dark ages ('60s), we DID use parafin for shroom/spore, long-term storage!I must also thank Prof. Wilkenssen (I always suspected he was "sporing" more than shitakes) ------------------
<<<~~~~~~~~~~>>>
. . . and all the people gathered in their secret place, deep in the forest, to share the wondrous fruits of the roots . . . . . . and they knew the glory of being.
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Anonymous
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#91531 - 01/08/00 11:41 AM (24 years, 8 months ago) |
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this of course means that dr stamets book is full of shit!---the mineral oil nonsense is from HIS BOOK! figures...
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 Nickthetrip
Super Saiyan
Registered: 06/11/02
Posts: 65
Loc: Ever tryed this position ...
Last seen: 21 years, 9 months
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: aaron]
#673176 - 06/11/02 11:44 AM (22 years, 3 months ago) |
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I tryed to quick freeze mycelium using a CO2 quick release gun while the mycelium was in a container and sealed it and threw into a freezer
I hope that it wouldn't damage the tissue of the mycelium thats why I froze it in a 5second period with the gun but I prayed...and it worked somehow I dont know how but after thawing it was able to produce fruit of couse I have no control on this experiment (as in years) but hey if it worked maybe it'll last years to come as long as it is in a sorta air tite vaccum container... If you have any questions about this or comments please reply!!!
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"Crawling in my skin, these wounds they will not heal, fear is how I fall, confusing, confusing what is real!."-Linkin Park
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Orchidman
enthusiast
Registered: 04/01/02
Posts: 205
Loc: Toronto
Last seen: 21 years, 10 months
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Re: Experiments in Storeing Cultures Under Mineral Oil [Re: Nickthetrip]
#675865 - 06/12/02 11:06 PM (22 years, 3 months ago) |
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I believe that once the mycellium is frozen with the CO2 gun it can last for a very long time frozen. The damage in freezing occurs when the water in the mycellium reaches 32f and at this temp the water expands and bursts the cells. I am curious as to why the cells did not burst as the mycellium thawed and was exposed to a much longer period of 32f than the quick freeze. I think the praying must have done it.
The damage in the freezer is from drying so an airtight container is a must.
Maybe you will develope a strain of freeze tolerant Cubensis or Pan tropicalis to grow in northern gardens--just wishing.
--------------------
I've been having psilly dreams lately
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