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OfflinePsilowarrior
enthusiast
Registered: 11/21/99
Posts: 195
Last seen: 21 years, 1 month
Re: Old cultures restarted
    #91007 - 12/14/99 01:35 PM (22 years, 7 months ago)

Funny I logged on to ask almost the same question. If I may also add another. When do you need to start worrying about senescence? Is it a phenomena that happens after only a few agar transfers or only after very many.


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Anonymous

Re: Old cultures restarted [Re: Psilowarrior]
    #91009 - 12/16/99 08:57 AM (22 years, 7 months ago)

senesence?????????????


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Anonymous

Re: Old cultures restarted [Re: Psilowarrior]
    #91010 - 12/16/99 11:59 AM (22 years, 7 months ago)

I've had a very hard time using cultures over 4 months old, probaly due to improper fridge procedures. Seems like anything I get to grow from something that old ends up growing super slow, 90% or better going down to containmation. I just started with some 5 month old cultures, 1 jar out of 25 made it. Which was enough to get a spore print and get another 50 going. Be VERY careful with your sterile techniques, since the growth may be slow. Let me know how it goes.
-Capt'n


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,148
Loc: my room
Last seen: 6 days, 15 hours
Re: Old cultures restarted [Re: Psilowarrior]
    #91011 - 12/16/99 12:29 AM (22 years, 7 months ago)

senescence.... :icons/icon2: :icons/icon19:

[This message has been edited by Anno (edited December 16, 1999).]



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OfflineDan
newbie
Registered: 05/11/01
Posts: 187
Last seen: 20 years, 8 months
Re: Old cultures restarted [Re: Psilowarrior]
    #91012 - 12/16/99 03:05 PM (22 years, 7 months ago)

Actally, from fully colonized petris from april, they sure are fast colonizers. I innoculated from them onto PDA on last sunday, and 32 hours later they were alive and VERY well. Now they are about 1/2 colonized. (I'm using baby food jars) only 1 spot of contamination out of 19 jars! I am amazed at these results, actually.


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Offlinephree_1
Stranger
Registered: 06/03/01
Posts: 64
Last seen: 21 years, 1 month
Re: Old cultures restarted [Re: Psilowarrior]
    #91013 - 12/16/99 09:03 PM (22 years, 7 months ago)

A friend and I transfered some cultures we had in baby food jars since 1994. We stored half the jars with mineral oil and half with nothing. They all survived. They are as vigorus as they ever were.


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Invisibleanandam
skrat

Registered: 10/29/99
Posts: 22
Re: Old cultures restarted [Re: Psilowarrior]
    #91014 - 12/17/99 02:52 AM (22 years, 7 months ago)

Sorry could someone tell me about mineral oil seal tek.


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Anonymous

Re: Old cultures restarted [Re: Psilowarrior]
    #91015 - 12/17/99 12:13 AM (22 years, 7 months ago)

Well, here's something I worked on some time ago, and pretty much perfected it.]
This is to for long time storage of agar cultures. When you prepare agar use only powders, no liquids, no water boiling, etc.
Now, take the powder of agar (Lets say Malt Agar powder), and use instead of water glycerin. Glycerin has a tendency to become like glass, and not to freeze. So, when you have a fully grown agar culture, you ca store it at low temps (0 C. and below), with no fear of it dying. But when you revive it, it must be transfered to a regular agar culture. let it defrost in room temp, and give it lots of light. When completly defrosted, put it in a temp of 27 F. for 2 days. This should give it its vitality back.

Later,
tweedy.



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