Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Petri Dish

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
agar transfers
    #2126135 - 11/20/03 11:01 PM (13 years, 17 days ago)

sorry this is double post and not terribly advanced but yielded mediocre replies in regular forum and was reccomended to be moved here.


after the dishes have been innculated with live tissue, how much growth is neccesary before it should be transferred? Heard that the first dish is most likely to hold contaminants and it take a few transfers before eliminating them. Mycofile's H202 tek was used. Does the growth need to cover the entire plate before being transferred? When transferring, how much should be sliced and should just the mycelium, not the tissue be transferred?


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCultyVader
Dark SporeApprentice

Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Re: agar transfers [Re: thisone]
    #2126149 - 11/20/03 11:08 PM (13 years, 17 days ago)

IM not positive, but i believe the answers to your questions are very subjective. Especially when you are dealing with contaminates, because at times they can grow faster than the mycelium. As far as how much to transfer, i believe even they tiniest amount will restart growth in a new dish. The whole point of transfering is to get specific types of growth. Find the part of your petri dish that has the most rhizomorphic growth, then cut that out. Dont cut too much because there is no point in bring some non-rhizomorphic growth. . . i think. Oh and you just need the mycelium, not the tissue


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: CultyVader]
    #2126165 - 11/20/03 11:19 PM (13 years, 17 days ago)

see, the thing is that I'm not dealing with contams nor do I think I will since the H202. Also won't it be isolated since it was from live tissue, it already has the genetic make-up as a fruiting strain. Is the sepeartion of rhizo and tomatose still important?


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCultyVader
Dark SporeApprentice

Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Re: agar transfers [Re: thisone]
    #2126238 - 11/20/03 11:47 PM (13 years, 17 days ago)

Theres a post somewhere a while back about "tomatose" (i think thats the wrong spelling) use search and check it out. I would think that seperating rhizo growth is always a good thing. If you see different forms of growth, grab the healthiest and most vigorous.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleautomanM
blasted chipmunk
 User Gallery

Registered: 09/18/03
Posts: 7,862
Trusted Cultivator
Re: agar transfers [Re: CultyVader]
    #2126256 - 11/20/03 11:53 PM (13 years, 17 days ago)

i just transfer the rhizos to other plates when i notice they are looking healthy. i transfer about 1/2 sq.in.


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: automan]
    #2126345 - 11/21/03 12:21 AM (13 years, 17 days ago)

so the entire plate need not be fully colonized? Is there any benefit in a transfer if contams are not an issue? Numerous gills were used to innoc a dish and have good growth. Should I just wait until the entire dish is colonized and could I just use that to innoc grain?


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCultyVader
Dark SporeApprentice

Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Re: agar transfers [Re: thisone]
    #2126378 - 11/21/03 12:33 AM (13 years, 17 days ago)

The only benefit of letting it fully colonize is to have more little agar wedges to inoculate with. That, and when you open the dish, if it is colonized then it will be harder to get contaminated when the cover is off.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: CultyVader]
    #2126384 - 11/21/03 12:35 AM (13 years, 17 days ago)

so it is worth it to let it fully colonize?


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleHongosmeester
old sourdough
Male User Gallery
Registered: 12/12/02
Posts: 246
Loc: East Bay (EAST BAY GREASE...
Re: agar transfers [Re: thisone]
    #2126393 - 11/21/03 12:39 AM (13 years, 17 days ago)

Quote:

thisone said:
so it is worth it to let it fully colonize? 



I think that it is :thumbup:


--------------------
Don't fuck with me
I'm a postal worker;-)
A zen master once said to me
don't do anything I tell you to do
so i did

<p><a href="http://www.whump.com/dropbox/other/ujname.html">Get yours</a>.</p>

<p>My <a href="http://tinyurl.com/6valr">Unitarian Jihad Name</a> is: <strong>Brother Neutron Bomb of Compassion</strong>.</p>
<p><a href="http://www.whump.com/dropbox/other/ujname.html">Get yours</a>.</p>


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: Hongosmeester]
    #2126400 - 11/21/03 12:42 AM (13 years, 17 days ago)

sweet!


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSam66
Stranger
Registered: 10/07/03
Posts: 3
Last seen: 12 years, 5 months
Re: agar transfers [Re: thisone]
    #2126716 - 11/21/03 05:54 AM (13 years, 17 days ago)

I wouldn't let it entirely colonize. Transfer before growth reaches the edges, as contamination is most likely to occur around the edge of the dish.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePaid
Pict
 User Gallery

Registered: 03/13/03
Posts: 5,376
Loc: Zone ate
Last seen: 12 years, 9 months
Re: agar transfers [Re: Sam66]
    #2126798 - 11/21/03 08:38 AM (13 years, 17 days ago)

Agreed. I start the first transpheres when
there is enougth mycleium for 4 new plates.
so when its the size of the hole when you join your
thumb and forefinger, its big enougth to start.
Take from the outside egdes ( but not
as has been already mentioned, from any edges that
have reached the petri dish walls)and avoid the center,
were the clone tissue is, as it can harbour contams
that are just taking longer to show up.

When transphering wedges, the size isnt really an issue,
as long as you transphere some colonised agar, it will spread
out from that point, imo less is better, as you have a better chance of it being cleaner.

I also flip the wedge upside down onto the new
plate, that way any contams than may have
landed on the mycelium surface, have a harder chance
to germinate, as their face down on h2o2 agar :]


--------------------



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: Paid]
    #2127078 - 11/21/03 12:31 PM (13 years, 17 days ago)

well, hey thanks. thats's very informative. I think I'm at that point now, so should four new dishes be made, or more? Since it is live tissue and the strain has been isolated already, is the only point of transferring to provide more healthy mycelium to use in the future? Also, again sorry for not being terribly advanced it's just that response level in regular forum is very limited in regards to agar. Looking for some informationon best way to make slants, and what tissue containers are for (I'm guessing to hold the tissue in place while storing long term), and what's the benefits of each?


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemickywilliams2
journeyman
Registered: 05/27/03
Posts: 135
Last seen: 2 years, 2 months
Re: agar transfers [Re: thisone]
    #2127233 - 11/21/03 01:29 PM (13 years, 17 days ago)

I have a question also.
When you inoc agar with fruit tissue will the growth always be rhizo?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 10 days
Re: agar transfers [Re: mickywilliams2]
    #2127297 - 11/21/03 01:53 PM (13 years, 17 days ago)

No.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinewhitesox
newbie
Registered: 01/31/03
Posts: 44
Last seen: 12 years, 7 months
Re: agar transfers [Re: Anno]
    #2128109 - 11/21/03 08:20 PM (13 years, 16 days ago)

anno=moron


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 10 days
Re: agar transfers [Re: whitesox]
    #2128120 - 11/21/03 08:24 PM (13 years, 16 days ago)

If you do?t have anything to say, then don?t say it.
Mmkay?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineErik006
MushroomCultivator

Registered: 07/13/03
Posts: 310
Loc: Ontario
Last seen: 6 years, 4 months
Re: agar transfers [Re: Anno]
    #2128276 - 11/21/03 09:51 PM (13 years, 16 days ago)

>anno=moron

don't be so quick to offend people, I'm sure anno doesnt mind, but you just dont say stuff like that around here, and especially to a well respected member like anno.

Erik006 :sun:


--------------------
At last you know what ineffable is, and what ecstacy means


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 11 years, 8 months
Re: agar transfers [Re: Erik006]
    #2128364 - 11/21/03 11:00 PM (13 years, 16 days ago)

okay, whitesox, really don't appreciate it because one, it is disrespectful and two, it had nothing to do with the most recent question I posted.


--------------------
Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCultyVader
Dark SporeApprentice

Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Re: agar transfers [Re: whitesox]
    #2128525 - 11/22/03 01:17 AM (13 years, 16 days ago)

THIS is exactly what happens when you close OTD, all the wierdos start coming out of the woodwork.. . .

Edit: not that he actually posted in OTD. . . . ever. .


Edited by CultyVader (11/22/03 01:18 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Agar transfer xburn 887 3 05/10/05 12:01 AM
by xburn
* Agar transfer question Dex_Detrimental 814 3 06/12/02 07:51 PM
by Dex_Detrimental
* Agar Transfers CultyVader 1,173 12 04/10/03 11:44 PM
by CultyVader
* Agar: Isolation from molds... ATWAR 4,629 13 07/24/04 12:27 AM
by ATWAR
* First Agar Growths Help Me ID! :) *DELETED*
( 1 2 all )
Nikon 2,035 31 02/14/09 01:53 AM
by WhAcKeD
* Agar problem (too sweet?) Dem_Bones 1,130 3 12/27/07 10:22 AM
by RogerRabbit
* Is this a 'non-sectoring' agar isolate? Aeolus1369 5,933 11 12/01/09 12:24 AM
by MasFina
* Should inoculation points touch on agar? Culland 601 2 11/02/07 08:26 AM
by Culland

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,861 topic views. 1 members, 14 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mycohaus
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.04 seconds spending 0.002 seconds on 16 queries.