Transferring contams away from the mycelium

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Spores for European Microscopy

Golden Teacher Liquid Culture For Sale

Substrate Bags

High THC Strains

Jump to first unread post

Pages: < Back | 1 | 2 [ show all ]

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

starspawn2
Prince of Prints

Registered: 02/13/04
Posts: 21
Loc: St,louis MO
Last seen: 6 years, 9 months

Re: Transferring contams away from the mycelium

[Re: RogerRabbit]
#4113872 - 04/30/05 06:53 AM (21 years, 3 months ago)

Quote:

heres what i think. Every time you transfer you are isolating, you will be isolating untill you are down to 1 mycelium and then you would be cloning

If you start with spores cuting out even the smallest piece of mycelium is not isolating.The only way to be 100% sure is to start with a tissue from a fruit.Just expose the mycelium to light and wait for it to start pinning,it will look like bumps on the agar.Cut a small piece of the bump and transfer it to a new dish.That is the fastest way to get a isolate.

Quote:

You always run the risk of isolating a non-fruiting strain

If you use a tissue to clone the isolate will always be a fruiting stain.

Quote:

this tells me that it is probably best to start multispore in a liquid culture

No,because sectors can't be picked out or even seen.

Extras:

Top

starseed1066
officially hosed

Registered: 04/13/05
Posts: 325
Loc: close enough to get mysel...
Last seen: 14 years, 2 months

Re: Transferring contams away from the mycelium

[Re: RogerRabbit]
#4114014 - 04/30/05 08:31 AM (21 years, 3 months ago)

Quote:

RogerRabbit said:Do this in a glove box and not in front of a flow hood. A flow hood will blow the contaminant spores everywhere.

you could always do it in front of a shop vac.. :laugh:

--------------------
Under his instruction, I taped drumsticks to his head and turned him into a sort of mummy, or perhaps a caterpiller in a cocoon. He remains this way for about three hours, making bizarre noises, pretending to be a new species that must learn to walk and talk and eat, etc. And he communicates with other life forms by way of the antennae on his head. Eventually we jam a tube from a waterbong into it's mouth and figure he's learned all he needs to know.