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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Transfer using casing section.
    #2424068 - 03/12/04 03:07 PM (12 years, 8 months ago)

Hey guys..

Here's a question. I have an isolate that's been going for about a year now, last august i baught a house and it has well water, took me a while to find out, but another hobby brought it to my attention  :smirk:

ph: 9.2
TDS: 480  :shocked:

Ever since moving i had been having a hard time getting my g2g workin right. the jars would colonize about 75 % and stall, the bottoms would not finish and appeared dark/almost black and end up contamed.

Of the few jars that survived, casings made with the same water/straw/compost would fruit very slowly and have tiny heads with rosecombs and would not mature correctly....... the addition of oyster shells in the casign helped a bit...

I now have an RO ( reverse osmosis ) and my water comes out ph 7.1 and TDS 0  :smile: made a batch of jars and they look MUCH better.

Now, i'm out of jars to transfer from, except 1 that's possibly usable...

I have a couple casings that were made with RO water and they fruited just fine !

Could i take a chunk from a fruiting casing and transfer it to good jars with any hope of no contamination? or would i be better off taking a section of a fresh mushroom and transfer with it ?

I assume that the mycelium of the casing and the mushroom stem are both the same isolate ??????

ok. this is getting long.. i'll shut up now !!  :grin:


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Transfer using casing section. [Re: PooPs]
    #2426325 - 03/13/04 06:24 AM (12 years, 8 months ago)

pH of 9.2 sounds crazy for any kind of drinking water, assuming your well water is intended as palatable.

You will probably have better luck with a tissue transfer. If you do use part of a casing, try to get a chunk from the center of the casing as this area remains sterile for much longer than the peripheria of the casing.

I think you will have trouble getting a straight answer regarding the genetics from the casing and fruits.

I do appreciate your experience though as I assumed most palatable water supplies to be fine for cultivation.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Re: Transfer using casing section. [Re: Joshua]
    #2432441 - 03/14/04 09:50 PM (12 years, 8 months ago)


Befoer Reverse osmosis:





After R.O.




--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Re: Transfer using casing section. [Re: PooPs]
    #2432449 - 03/14/04 09:53 PM (12 years, 8 months ago)

I was getting PH 7.0 for a while, but i have not flushed the membrane in a few weeks, my PPM reading changes by + or - 30 at different times..

The water is high in manganese, ( leaves a black film on sink and bathtub... and smells like shit, but otherwise clear to the eye )


:beer:
:spliff:


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Transfer using casing section. [Re: PooPs]
    #2441940 - 03/17/04 03:39 AM (12 years, 8 months ago)

I want some cool toys! Maybe someday I'll have some money to buy some.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 7 months
Re: Transfer using casing section. [Re: Joshua]
    #2443075 - 03/17/04 01:38 PM (12 years, 8 months ago)

Toys of the trade  my friend !  :wink:

Gotta keep them fish happy !


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


Post Extras: Print Post  Remind Me! Notify Moderator
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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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