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InvisibleCLuB99
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Re: Liquid mycelium and glycerine ( Dr. Dung, look at this)
    #91197 - 12/19/99 01:55 PM (22 years, 9 months ago)

Opppsssss!!!
I use to colonize my jars with a liquid culture made with honeywater


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Anonymous

Re: Liquid mycelium and glycerine ( Dr. Dung, look at this) [Re: CLuB99]
    #91199 - 12/19/99 11:20 PM (22 years, 9 months ago)

I've thought about trying to freeze living mycelium like that, but I've never done it. I think it could work.

The glycerin shouldn't hurt your spore germination at all. Glycerin is a food substance to animals, part of fat molecules. I don't know if fungi can digest it, but it doesn't hurt at reasonable concentrations.

Try it and report back. If I was going to try it, I would get the mycelium water growing, then add 10% sterilized glycerin a few hours before I popped it in the freezer.

DD



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InvisibleCLuB99
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Registered: 10/27/99
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Re: Liquid mycelium and glycerine ( Dr. Dung, look at this) [Re: CLuB99]
    #91200 - 12/20/99 04:35 PM (22 years, 9 months ago)

Tnx Dok!!


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Anonymous

Re: Liquid mycelium and glycerine ( Dr. Dung, look at this) [Re: CLuB99]
    #91201 - 12/21/99 03:02 AM (22 years, 9 months ago)

Actually glycerin is a water substitute in cryogenic studies because it tends to crytallize (become like glass) instead of freezing, becoming like ice, thus breaking the cell's structure. I have done with succes, but I have found one thing. After a period of freezing, the cultures need to be revived in some way - first let it heat on its own, next give it a lot of light for 2 days, then move to a new medium, so it can continue its growth as a healthy mycelium...

Later,
tweedy.



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