Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!
I've thought about trying to freeze living mycelium like that, but I've never done it. I think it could work.
The glycerin shouldn't hurt your spore germination at all. Glycerin is a food substance to animals, part of fat molecules. I don't know if fungi can digest it, but it doesn't hurt at reasonable concentrations.
Try it and report back. If I was going to try it, I would get the mycelium water growing, then add 10% sterilized glycerin a few hours before I popped it in the freezer.
Actually glycerin is a water substitute in cryogenic studies because it tends to crytallize (become like glass) instead of freezing, becoming like ice, thus breaking the cell's structure. I have done with succes, but I have found one thing. After a period of freezing, the cultures need to be revived in some way - first let it heat on its own, next give it a lot of light for 2 days, then move to a new medium, so it can continue its growth as a healthy mycelium...