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Offlinecarnage
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Registered: 02/25/03
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storing liquid medium
    #2062991 - 11/01/03 05:36 PM (13 years, 1 month ago)

I am setting up a lab for myself and I want to make all my cultures and all my medium jars at once. I know it is possible to store my cultures without any harm done, but am I able to prepare and sterilize liquid medium jars (using malt extract and distilled water) and store them for later use? If I am able to store them, how would I go about doing so and how long would I be able to store them?


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Invisiblemicro
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Re: storing liquid medium [Re: carnage]
    #2063169 - 11/01/03 06:48 PM (13 years, 1 month ago)

It's a better idea to make them fresh. They'll eventually die in the fridge. You could try freezing them, but the freezing might lyse the cells; adding DMSO might help this.

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Offlineragadinks
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Re: storing liquid medium [Re: micro]
    #2063394 - 11/01/03 08:11 PM (13 years, 1 month ago)

You did it again ! :lol:
What is  "DMSO"


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Invisiblemicro
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Re: storing liquid medium [Re: ragadinks]
    #2063866 - 11/01/03 11:48 PM (13 years, 1 month ago)

Sorry! Dimethyl-sulfoxide. It's a common laboratory reagent, or it can be ordered from http://www.dmso.com

People use it in about a 50/50 concentration with water when freezing cells to prevent ice crystals from forming, which can lyse cells really easily.

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Offlineragadinks
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Re: storing liquid medium [Re: micro]
    #2064192 - 11/02/03 03:51 AM (13 years, 1 month ago)

Thanks for you patience ...  :frown: 


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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