Home | Community | Message Board


The Best Salvia
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PureVPN PureVPN   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   NordVPN NordVPN   Amazon Agar, Microscope, Paul Stamets

Jump to first unread post. Pages: 1
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91389 - 12/28/99 04:14 PM (17 years, 5 months ago)

I dont think colchicine does this, but I might be wrong. Actually colchicine is used to mutate canabis seeds to be only female seeds (=more THC). It will create new strain but will probably will not cross breed any strains. You know, thats how most of Mr. G's strains were created. Taking colchicine, applying to spores, looking for germination under a microscope (only a few spores will germinate after contact with colchicine), moving these to new agars, stabilizing the new strain and than voila! a new strain is created.
If you would like to cross breed two strains you would need a mutagen that takes one nucleum frm a cell and putin it in a different cell, thus mixing the gene pool together, and croos breeding a strain out of two different species.

Later,
tweedy.



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91391 - 12/28/99 05:01 PM (17 years, 5 months ago)

Actually, it is true that when you induce tetraploid in two incompatable species/strains in the same genera that they can be breed together to produce a fertile amphidloid. I've been doing alot of research on colchicine and induced polyploidy plants, lately. Althought, I don't know why it would produce all female plants but I remember seeing something like this before.

-PsiliPharm



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91392 - 12/29/99 01:50 PM (17 years, 5 months ago)

I have thought about this for a long time but never done it (no colchicine or autumn chrocus). I think you would have to isolate monokarytoic mycelium. Transfer monokaryotic mycelium of the two different species onto an agar plate w/ colchicine in it and allow the monokaryots to grow towards eachother. When the to myceliums meet mating may occur. Transfer mycelium from the meeting point onto a new plate w/o colchicine. If growth is strong a new hybrid may have been created. Now you need to try to fruit it.
Here is the hybrid I wanted to see happen:
Ps. rogusa-annulata (the garden giant, up to 5lbs each)x Ps azur :wink:

Check out www.fungiperfecti.com for pictures (and correct spelling of rogusa-annulata



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91393 - 12/29/99 01:52 PM (17 years, 5 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91394 - 12/29/99 04:19 PM (17 years, 5 months ago)

Ok, I don't know if the garden giant will work. And it's not a Psilocybe species and doesn't contain Psilocin/Psilocybin. It is Stropharia rugoso-annulata which Psilocybe cubensis was once called Stropharia cubensis but these still may not be compatable but it would be interesting to see.

-PsiliPharm



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91395 - 12/30/99 10:04 AM (17 years, 5 months ago)

The garden giant has been reclasified as a psilocybe according to Stamets himself. This does not mean that it contains psilocybin (it doesnt). The genes for psilocybin production would come from azur and the genes for size from the gg, hopefully. Anyway, stropharia and psilocybe are very closely related and many mycologist have suggested combining them. Dont forget just because we classify something as species a doesnt mean much anyway. Our classifiaction can be very artificall. This combination has about as good a chance at working as the other.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91396 - 12/30/99 10:09 AM (17 years, 5 months ago)

BTW, just for an example for the long list above, Treasure Coast and B+ are not 2 different species (they are 2 different strains)and could simply be mating by isolating monokarytic mycelium. No colchicine is need. That pretty much applies to most of your list. If you happento buy into the idea that B+ is not a cube than take Gulf Coast and Phantasia Mystery as an example of 2 different STRAINS of cubensis.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTweeky1
Stranger
Registered: 08/26/99
Posts: 1
Re: Creating New Strains of Psychedelic Mushrooms
    #91397 - 01/12/00 11:24 PM (17 years, 5 months ago)

Man could you imagine if that big sucker had the good stuff in it!!!! If you grew one and dried it you would be set for at least a year!@!@!@!@!@!

I would pay mega bucks for a strain like that!



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMycelium5150
Check theDate!!!!
Registered: 04/06/99
Posts: 541
Last seen: 12 years, 3 months
Re: Creating New Strains of Psychedelic Mushrooms
    #91398 - 01/12/00 11:33 PM (17 years, 5 months ago)

what a waste of time, how long did it take you to type out that list of cross's. come on, each one of those wqould have to be tested. even more time consuming


--------------------
Mad skills, you know this!!!

I am here to Myth Bust


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91399 - 01/13/00 11:38 PM (17 years, 5 months ago)

Hehe...but I like consuming...

-PsiliPharm



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Creating New Strains of Psychedelic Mushrooms
    #91400 - 01/16/00 06:08 AM (17 years, 5 months ago)

once again, all colchicine is useful for is to induce polyploidy in living cells and killing most of the others. It doesn't make all female pot, I have seen plenty of polyploid males, females and hermies. It doesn't magically allow cross-breeding. Some polyploid strains/species may get new breeding abilities, but it is not a given. It's not even likely, it's just possible, due to new genetic material(new as in quantity not quality).
Example, once again: ever eaten a strawberry? Did it come from a supermarket? If so it was most likely a polyploid strawberry. Polyploidy in plants often produces larger more hardy plants. Ever eaten an apple? Same deal as the strawberries. Now, ever eaten a strapple? How about an apperry? Didn't think so. If you ever do, it will likely have been created through gene splicing, possibly polyploid gene splicing, probably not. The polyploid state would be much easier to create after you've already crossed the strawberry and apple genes. Point is, polyploidy doesn't automatically allow cross-breeding, especially across species or family lines. All it does is double the number of chromosomes, plain and simple. Interesting effects, but probably not the magic cure all that some pipe dreams might lead you to believe.
Remember how poisonous this stuff is. Don't mess with it unless you are prepared to die.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Creating New Strains of Psychedelic Mushrooms
    #91401 - 01/16/00 01:54 PM (17 years, 5 months ago)

Well, I know that you can't cross an apple with a strawberry, because they aren't even in the same genus. Most of the strains listed above are the same species just different strains, but all of them are of the same genus. They are all Psilocybe mushrooms, it is very likely that these cross-strains will be but of course the Stropharia species mentioned before is not so likely. Some gene-splicing would probably have to be done to acomplish this. I just started this thread to spark people's thinking and yes, colchicine is dangerous, don't use it unless you know what you're doing. You practically have to have a suit like you would see those guys on TV who are dealing with some kind of nuclear waste or biological virus. It might as well be put into this catagory since it requires pretty much the same type of protection.

-PsiliPharm



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSclorch
Clyster

Folding@home Statistics
Registered: 07/13/99
Posts: 4,805
Loc: On the Brink of Madness
Re: Creating New Strains of Psychedelic Mushrooms
    #91402 - 01/18/00 05:17 PM (17 years, 5 months ago)

An easy way to describe colchicine is "The Poor Man's Genetic Engineer". Seriously, don't f**k around with it.


--------------------
Note: In desperate need of a cure...


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: PureVPN PureVPN   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   NordVPN NordVPN   Amazon Agar, Microscope, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Psilocybe mexicana, strain A mushrooms
( 1 2 all )
WorkmanV 8,402 28 03/03/04 12:36 PM
by PsiloSteve
* psychedelic mushrooms and developing nations - a new chapter in the relation between shrooms and man AsanteA 1,050 4 02/01/06 07:01 AM
by blackout
* Re: Genetically Mutated Mushrooms
( 1 2 3 all )
mycofile 9,287 47 12/28/99 02:48 PM
by Anonymous
* Has Anyone Created/Isolated Their Own Mushroom Strain? LogicaL Chaos 3,905 18 01/16/11 06:58 PM
by yummytrips
* Would this Work for Creating Hybrid Strains??
( 1 2 3 all )
HerbBaker 9,370 41 03/06/13 02:13 PM
by circuspimp
* Creating Genetic Hybrids Through Electroporation?
( 1 2 all )
AdoreChampignons 3,857 31 10/29/08 05:10 PM
by fastfred
* creating new strains?
( 1 2 all )
Cyber 4,708 36 07/30/04 01:30 PM
by fastfred
* Getting started with Strain Isolation IcAnFeeLthErain 2,422 12 10/31/08 07:59 AM
by HerbBaker

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
6,909 topic views. 0 members, 4 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
World Seed Supply
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.003 seconds on 15 queries.