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Offlinef8L
Triumph

Registered: 09/07/02
Posts: 397
Last seen: 11 years, 20 days
MOST important aspect to PFTEK jars!
    #898332 - 09/21/02 08:20 PM (21 years, 6 months ago)

Not sure where this belongs, but it hasn't been mentioned nearly enough!

For all the newbies out there following the PFTEK 1/2 pint jar tek-- When you are innoculating, make a WIDE and DEEP sweeping motion with the syringe to cover as much space as possible in each of the four innoculation points.

I cannot tell you how much this helps! A lot of people experienced stalled jars, I believe the reason for this is because they simply push the syringe down and innoculate, leading to longer incubation and colonization times, leading to a buildup of co2.

Within the first 7 days I noticed about a 3x increase compared to the jars that had not been 'sweep innoculated.'

Just a tip.

Edited by f8L (09/21/02 08:21 PM)

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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 21 years, 4 months
Re: MOST important aspect to PFTEK jars! [Re: f8L]
    #898342 - 09/21/02 08:24 PM (21 years, 6 months ago)

thanks for the tip. ill try that method in 2 of the 4 jars and note the results :smile:

will that increase chance of contams? do I sweep in a 360 degree motion, like move the needle around as I drip spores?

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Offlinef8L
Triumph

Registered: 09/07/02
Posts: 397
Last seen: 11 years, 20 days
Re: MOST important aspect to PFTEK jars! [Re: LSAuser]
    #898391 - 09/21/02 08:47 PM (21 years, 6 months ago)

No... just along the glass... basically what you are trying to do is to spread the spores along as much of the substrate as possible... I think people worry about contams too much-- you should be fine.. good luck

Edited by f8L (09/21/02 08:54 PM)

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Offlinezazz
mini F+

Registered: 05/28/02
Posts: 294
Last seen: 19 years, 2 months
Re: MOST important aspect to PFTEK jars! [Re: f8L]
    #898451 - 09/21/02 09:25 PM (21 years, 6 months ago)

not sure if this is what you mean, but i kinda drag it up as i push down. but usually mine dont flow as easily so it just comes out in a big squit which spreads it out pretty evenly. one time i had growth spots about an inch or 1/2 inch away from the innoc point.

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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: zazz]
    #898609 - 09/21/02 10:54 PM (21 years, 6 months ago)


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 19 years, 14 days
Re: MOST important aspect to PFTEK jars! [Re: matts]
    #898663 - 09/21/02 11:42 PM (21 years, 6 months ago)

Damn this post made me feel so dumb, never thought of that, I mean I've read a whole lot, maybe I didn't read carefully enough. Good job. Thanks f8L. 2 shrooms to you.


--------------------
Re-Defeat Bush in '04

Edited by Skikid16 (09/21/02 11:45 PM)

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Invisibletekramrepus
Female User Gallery
Registered: 02/20/02
Posts: 2,253
Re: MOST important aspect to PFTEK jars! [Re: Skikid16]
    #899212 - 09/22/02 12:03 PM (21 years, 6 months ago)

good post f8l!

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OfflineLost_Prophet
Mr Red Eye
Registered: 09/21/02
Posts: 17
Loc: Another astral plain
Last seen: 20 years, 2 months
Re: MOST important aspect to PFTEK jars! [Re: tekramrepus]
    #899262 - 09/22/02 12:41 PM (21 years, 6 months ago)

if only I'd read this post five minutes earlier :mad:. Oh well I'll just have to try it with my next batch, thanks for the tips 


--------------------
Right, who's buying the next round?

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