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 blackout
Registered: 07/16/00
Posts: 5,266
Last seen: 2 years, 1 month
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 has fractional sterilisation worked for anybody 
#852391 - 08/30/02 12:45 AM (23 years, 3 months ago) |
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i tried it a while back with whole wheat grains in a microwave and failed. mycelium did grow but weas taken over by green a week later.
i have a autoclave bag of wheat now which got contaminated i think the spore syringe must have been bad since i sterilised the wheat it properly. i nuked the bag in the microwave for 20mins to kill the green mould and have innoced again with a vendor spore syringe which should be ok.
i did a search on resterilising contaminated substrates and workman (i think) said it should be ok but is probably not recommended. i read in a biology book that botulism (when growing/vegetating) and others are killed in 10mins at 100C.
i have also microwaved some more wheat (soaked for 24hr) for about 1/2 hour. this time i will wait until i see contamination before i microwave them again. i will do this 4 or 5 times.
has anybody ever tried this or know if im wasting my time
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 2 months, 2 days
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#852399 - 08/30/02 12:57 AM (23 years, 3 months ago) |
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>has fractional sterilisation worked for anybody
For me, but not using the microwave, rather boiling in a pot.
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blackout
Registered: 07/16/00
Posts: 5,266
Last seen: 2 years, 1 month
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Re: has fractional sterilisation worked for anybody [Re: Anno]
#852446 - 08/30/02 02:49 AM (23 years, 3 months ago) |
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was it rye grain?
who many times did you do it (individual heatings not the whole process)?
incubation period between heatings?
heating time?
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 Shaw
Registered: 06/27/01
Posts: 2,264
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#852584 - 08/30/02 05:04 AM (23 years, 3 months ago) |
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I've never had success with anything in the microwave
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killerjay
idiot savant
Registered: 10/26/01
Posts: 55
Loc: Wisconsin
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Re: has fractional sterilisation worked for anybody [Re: Anno]
#852804 - 08/30/02 07:21 AM (23 years, 3 months ago) |
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I was having problems with contamination a while back so I attempted it by boiling jars two and even three times, each a day apart. It did not work however with rye, millet, or brown rice flour cakes, so I eventually broke down and bought a 22qt presto pressure cooker for $80 and have had much better results since then.
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Cubies4Me
Child of theShroom
Registered: 05/11/02
Posts: 146
Loc: Another Realm
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#853011 - 08/30/02 09:18 AM (23 years, 3 months ago) |
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I used it with 3 jars of rye grain a month or so ago and it worked fine, jus too much work. I boiled it in a pot for a half hour and then let it incubate for 24 hours, boiled half hour again, incubate 24 hours and then boiled for an hour jus to be safe. I then bought a 16 qt. pc to make things easier. I have never tried with whole wheat or anything else. hope this helps.
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Livin comfortably numb, always and forever.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#853360 - 08/30/02 12:57 PM (23 years, 3 months ago) |
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HisStudent
enthusiast
Registered: 02/01/02
Posts: 206
Last seen: 17 years, 11 days
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#854139 - 08/30/02 09:51 PM (23 years, 3 months ago) |
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Your microwave sterilization doesn't mean shit unless you know the core temperature. A microwave thermometer is what you need. They can be had at most large chain stores such as target/k-mart/Wal-Mart.... you get the idea.
If no luck there, call up the gourmet kitchen places in town. Most malls have one these days. What you're looking for is a therm with no metal on it, they're pretty easy to find.......
When its all said and done, Getting conistant results in the microwave is a science and should be treated as such. But to make a long post short, Get them grains hot, 250 or better for atleest 10 minutes each time, and at least 3 times separated by at least an hour................
It's worked for me but I prefer a pressure cooker...
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I don't think I'm alone when I say I'd like to see more and more planets fall under the ruthless domination of our solar system.
- Jack Handey
Edited by HisStudent (08/30/02 09:52 PM)
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canid
irregular meat sprocket
Registered: 02/26/02
Posts: 11,946
Loc: looking for zeebras, n. c...
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Re: has fractional sterilisation worked for anybody [Re: blackout]
#854202 - 08/30/02 11:19 PM (23 years, 3 months ago) |
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i've had good luck with it using rye grass seed, but i boiled.
microwaves are not to be trusted, muahaha...
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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blackout
Registered: 07/16/00
Posts: 5,266
Last seen: 2 years, 1 month
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Re: has fractional sterilisation worked for anybody [Re: canid]
#858022 - 09/01/02 09:17 PM (23 years, 3 months ago) |
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i have a few substrates ready to be innoced soon. all were cooked in the microwave. i put the substrates in jars with filter lids or loose lids. then put them in an autoclave bag with some boiling water in it. i seal the bag with a large clip and put it in the micro. the bag expands and gets saturated with steam inside, it blows up like a ballon but doesnt pop since there is steam released through the filter patch. like a low pressure, pressure cooker in the microwave! so far the jars have looked nice with no dry spots in them.
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