Home | Community | Message Board


Sporeworks
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Capsules, Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Edibles   Amazon ½ Pint Jars, Autoclave, Brown Rice Flour, Hot Plate, Pressure Cooker, Rye Grain

Jump to first unread post. Pages: 1
Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,066
Last seen: 3 days, 16 hours
Trusted Cultivator
has fractional sterilisation worked for anybody
    #852391 - 08/30/02 02:45 AM (18 years, 8 months ago)

i tried it a while back with whole wheat grains in a microwave and failed. mycelium did grow but weas taken over by green a week later.
i have a autoclave bag of wheat now which got contaminated i think the spore syringe must have been bad since i sterilised the wheat it properly. i nuked the bag in the microwave for 20mins to kill the green mould and have innoced again with a vendor spore syringe which should be ok.
i did a search on resterilising contaminated substrates and workman (i think) said it should be ok but is probably not recommended. i read in a biology book that botulism (when growing/vegetating) and others are killed in 10mins at 100C.
i have also microwaved some more wheat (soaked for 24hr) for about 1/2 hour. this time i will wait until i see contamination before i microwave them again. i will do this 4 or 5 times.
has anybody ever tried this or know if im wasting my time


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,142
Loc: my room
Last seen: 4 hours, 29 minutes
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #852399 - 08/30/02 02:57 AM (18 years, 8 months ago)

>has fractional sterilisation worked for anybody
For me, but not using the microwave, rather boiling in a pot.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,066
Last seen: 3 days, 16 hours
Trusted Cultivator
Re: has fractional sterilisation worked for anybody [Re: Anno]
    #852446 - 08/30/02 04:49 AM (18 years, 8 months ago)

was it rye grain?
who many times did you do it (individual heatings not the whole process)?
incubation period between heatings?
heating time?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineShaw
hick

Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
Last seen: 5 months, 10 hours
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #852584 - 08/30/02 07:04 AM (18 years, 8 months ago)

I've never had success with anything in the microwave


--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinekillerjay
idiot savant
Registered: 10/26/01
Posts: 55
Loc: Wisconsin
Last seen: 11 years, 9 months
Re: has fractional sterilisation worked for anybody [Re: Anno]
    #852804 - 08/30/02 09:21 AM (18 years, 8 months ago)

I was having problems with contamination a while back so I attempted it by boiling jars two and even three times, each a day apart. It did not work however with rye, millet, or brown rice flour cakes, so I eventually broke down and bought a 22qt presto pressure cooker for $80 and have had much better results since then.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCubies4Me
Child of theShroom

Registered: 05/11/02
Posts: 146
Loc: Another Realm
Last seen: 17 years, 9 months
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #853011 - 08/30/02 11:18 AM (18 years, 8 months ago)

I used it with 3 jars of rye grain a month or so ago and it worked fine, jus too much work. I boiled it in a pot for a half hour and then let it incubate for 24 hours, boiled half hour again, incubate 24 hours and then boiled for an hour jus to be safe. I then bought a 16 qt. pc to make things easier. I have never tried with whole wheat or anything else. hope this helps.


--------------------
:tongue:
  Livin comfortably numb, always and forever.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,142
Loc: my room
Last seen: 4 hours, 29 minutes
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #853360 - 08/30/02 02:57 PM (18 years, 8 months ago)



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHisStudent
enthusiast

Registered: 02/01/02
Posts: 206
Last seen: 12 years, 5 months
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #854139 - 08/30/02 11:51 PM (18 years, 8 months ago)

Your microwave sterilization doesn't mean shit unless you know the core temperature. A microwave thermometer is what you need. They can be had at most large chain stores such as target/k-mart/Wal-Mart.... you get the idea.

If no luck there, call up the gourmet kitchen places in town. Most malls have one these days. What you're looking for is a therm with no metal on it, they're pretty easy to find.......

When its all said and done, Getting conistant results in the microwave is a science and should be treated as such. But to make a long post short, Get them grains hot, 250 or better for atleest 10 minutes each time, and at least 3 times separated by at least an hour................
It's worked for me but I prefer a pressure cooker...


--------------------
I don't think I'm alone when I say I'd like to see more and more planets fall under the ruthless domination of our solar system.
- Jack Handey


Edited by HisStudent (08/30/02 11:52 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinecanid
irregular meat sprocket
Male User Gallery

Folding@home Statistics
Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 8 months, 11 days
Re: has fractional sterilisation worked for anybody [Re: blackout]
    #854202 - 08/31/02 01:19 AM (18 years, 8 months ago)

i've had good luck with it using rye grass seed, but i boiled.
microwaves are not to be trusted, muahaha...


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,066
Last seen: 3 days, 16 hours
Trusted Cultivator
Re: has fractional sterilisation worked for anybody [Re: canid]
    #858022 - 09/01/02 11:17 PM (18 years, 8 months ago)

i have a few substrates ready to be innoced soon. all were cooked in the microwave. i put the substrates in jars with filter lids or loose lids. then put them in an autoclave bag with some boiling water in it. i seal the bag with a large clip and put it in the micro. the bag expands and gets saturated with steam inside, it blows up like a ballon but doesnt pop since there is steam released through the filter patch. like a low pressure, pressure cooker in the microwave! so far the jars have looked nice with no dry spots in them.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Capsules, Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Edibles   Amazon ½ Pint Jars, Autoclave, Brown Rice Flour, Hot Plate, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Re: microwave sterilization Anonymous 1,619 5 04/05/00 04:42 PM
by sparks
* fractional sterilization yogenfruzen 588 1 12/08/02 02:07 AM
by Anno
* rolled rye for substrate? jason890 508 1 01/13/02 10:22 AM
by Anno
* Bird Seed...can it be sterilised in the microwave or must PC du_Rag_scotland 993 7 02/10/03 06:06 AM
by MeltingPenguin
* Microwave sterilization of grain
( 1 2 all )
DarK_SavioR 11,809 35 01/08/17 06:24 AM
by Papa_Bear
* microwave & cboard spore germination: results comario2 2,180 14 10/24/02 02:55 PM
by comario2
* Worm castings as bulk substrate? Anyone have exp? Pugslee_Atoms420 6,236 19 08/28/10 12:51 AM
by RogerRabbit
* radioactive microwave information? BeppoMarx 824 3 10/18/01 12:34 PM
by BeppoMarx

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, bodhisatta
1,529 topic views. 0 members, 2 guests and 2 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Kratom Eye
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.005 seconds on 13 queries.