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Offlinearmyabe
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Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
can LC be grown in vacutainer?
    #7748852 - 12/12/07 11:24 AM (16 years, 1 month ago)

subject holds it all.


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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Offlineeman113
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Registered: 10/04/07
Posts: 85
Loc: il
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Re: can LC be grown in vacutainer? [Re: armyabe]
    #7749454 - 12/12/07 01:47 PM (16 years, 1 month ago)

Yes use search engine


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OfflineMYSTIQUE
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Registered: 04/28/07
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Re: can LC be grown in vacutainer? [Re: eman113]
    #7749695 - 12/12/07 02:40 PM (16 years, 1 month ago)

Thats what I use to grow mine in. By the time you got the dextros water and spores and what not in there its gonna have no vacume left anyways


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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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OfflineBikerfool
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Registered: 11/21/05
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Re: can LC be grown in vacutainer? [Re: MYSTIQUE]
    #7749886 - 12/12/07 03:37 PM (16 years, 1 month ago)

LC can't be grown in a true vacuum because there's no gas exchange. There needs to be some kind of filter which lets gases in and out of the jar. I read about vacu-tainers in a LC tek, I tried it and nothing grew. The "airport" tek may be a good technique to use with LC vacu-tainers.


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Just an angsty teen contributing to the pubs decline with contentless posts.


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OfflineMYSTIQUE
Say Hi to the elves for me.
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Registered: 04/28/07
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Re: can LC be grown in vacutainer? [Re: Bikerfool]
    #7750034 - 12/12/07 04:19 PM (16 years, 1 month ago)

You should add like air once a week to a vac or any other closed lc
Once you fill the vac with lc its more of a test tube with a rubber stopper then a vac .

flame Clean the needle and alcohol swab the cap then stick it in and try to suck out all the air you can. then suck air into your needle threw a flame and blow all the air you can into the tube.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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OfflineBreakfastClub
Don't you,forget about me

Registered: 10/23/07
Posts: 134
Last seen: 14 years, 10 months
Re: can LC be grown in vacutainer? [Re: MYSTIQUE]
    #7750578 - 12/12/07 06:18 PM (16 years, 1 month ago)

vacutainers are great way to store lc's and master cultures... but due to no gas exchange and low volume there is no point


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