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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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can LC be grown in vacutainer?
#7748852 - 12/12/07 11:24 AM (16 years, 1 month ago) |
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subject holds it all.
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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eman113
Stranger

Registered: 10/04/07
Posts: 85
Loc: il
Last seen: 14 years, 11 months
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Re: can LC be grown in vacutainer? [Re: armyabe]
#7749454 - 12/12/07 01:47 PM (16 years, 1 month ago) |
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Yes use search engine
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: can LC be grown in vacutainer? [Re: eman113]
#7749695 - 12/12/07 02:40 PM (16 years, 1 month ago) |
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Thats what I use to grow mine in. By the time you got the dextros water and spores and what not in there its gonna have no vacume left anyways
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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Bikerfool
Your Local Edgelord



Registered: 11/21/05
Posts: 1,577
Last seen: 5 months, 13 days
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Re: can LC be grown in vacutainer? [Re: MYSTIQUE]
#7749886 - 12/12/07 03:37 PM (16 years, 1 month ago) |
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LC can't be grown in a true vacuum because there's no gas exchange. There needs to be some kind of filter which lets gases in and out of the jar. I read about vacu-tainers in a LC tek, I tried it and nothing grew. The "airport" tek may be a good technique to use with LC vacu-tainers.
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: can LC be grown in vacutainer? [Re: Bikerfool]
#7750034 - 12/12/07 04:19 PM (16 years, 1 month ago) |
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You should add like air once a week to a vac or any other closed lc Once you fill the vac with lc its more of a test tube with a rubber stopper then a vac .
flame Clean the needle and alcohol swab the cap then stick it in and try to suck out all the air you can. then suck air into your needle threw a flame and blow all the air you can into the tube.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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BreakfastClub
Don't you,forget about me

Registered: 10/23/07
Posts: 134
Last seen: 14 years, 10 months
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Re: can LC be grown in vacutainer? [Re: MYSTIQUE]
#7750578 - 12/12/07 06:18 PM (16 years, 1 month ago) |
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vacutainers are great way to store lc's and master cultures... but due to no gas exchange and low volume there is no point
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