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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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OfflineWojo
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LC question, PICS
    #4294838 - 06/14/05 01:48 PM (12 years, 4 months ago)

I tried a lc baby bottle about a month ago, sterilized and innoc'd it. Now i have this




I tried using it to start jars a week or 2 ago (there was much less white shit than there is now), and got nothing. Should i try again now or wait? How much mycelia in the bottle do you need?


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Offlinelepiota
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Re: LC question, PICS [Re: Wojo]
    #4294844 - 06/14/05 01:52 PM (12 years, 4 months ago)

what did you mix to water? honey? karo? dextrose? and how much?


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OfflineWojo
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Re: LC question, PICS [Re: lepiota]
    #4294850 - 06/14/05 01:54 PM (12 years, 4 months ago)

Srry, KARO, 3 ml to about 200 ml h20


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Offlinelepiota
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Re: LC question, PICS [Re: Wojo]
    #4294870 - 06/14/05 02:05 PM (12 years, 4 months ago)

maybe you would have had a faster colonization using a little more karo (3% is said to be optimal, that is 6ml karo in 200ml water).
when you have some fluffy white floating in water it is ok to use for inoculation, just shake it hard first.
if your jars have no signs after one week it means that something was wrong. if i was in you i would inoculate them again
good luck


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OfflineWojo
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Re: LC question, PICS [Re: lepiota]
    #4294905 - 06/14/05 03:47 PM (12 years, 4 months ago)

Thxxx, how thick can the mycelia actually get in there?


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InvisibleRoadkillM
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Re: LC question, PICS [Re: Wojo]
    #4294964 - 06/14/05 03:58 PM (12 years, 4 months ago)

Quote:

Wojo said:

Srry, KARO, 3 ml to about 200 ml h20




thats too weak!~

4ml of karo to 96ml of h2o is a really good ratio.

:smile:


--------------------
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PM the names with on there names, that means they have mushrooms for sale.



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Offlinedjsage420
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Re: LC question, PICS [Re: Roadkill]
    #4295212 - 06/14/05 04:59 PM (12 years, 4 months ago)

Thats what my Lc's normally look like I just shake and Ive never had a problem with them I also Use 1/2cc per injection site, I use 2 tablespoons karo for 1 cup water... but listen to roadkill about the mix he has set me straight on a lot of mixing ratios


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Offlineblackout
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Re: LC question, PICS [Re: djsage420]
    #4298050 - 06/15/05 04:43 AM (12 years, 4 months ago)

You can make a thick solution of karo, sterilize and inject to bring it to 4%. And shake the jar to aerate


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OfflineCLUTCH
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Re: LC question, PICS [Re: Wojo]
    #4298365 - 06/15/05 10:07 AM (12 years, 4 months ago)

Well, since the karo solution was weak, I gotta ask...
and I'm suprised nobody has,


How much/many cc's of multispores did you inject??


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OfflineWojo
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Re: LC question, PICS [Re: CLUTCH]
    #4298374 - 06/15/05 10:11 AM (12 years, 4 months ago)

aboot 2 cc's


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OfflineCLUTCH
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Re: LC question, PICS [Re: Wojo]
    #4298387 - 06/15/05 10:18 AM (12 years, 4 months ago)

:thumbup:


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Offlinescatmanrav
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Re: LC question, PICS [Re: CLUTCH]
    #4298457 - 06/15/05 10:46 AM (12 years, 4 months ago)

>and I'm suprised nobody has

Because it doesnt really matter how many you put in...you only need a drop.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineCLUTCH
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Re: LC question, PICS [Re: scatmanrav]
    #4298479 - 06/15/05 11:03 AM (12 years, 4 months ago)

mmmmm, ok.  I'll go with that.  Got a point.

only NEED a drop, but wouldn't a couple cc's make it colonize faster?

If he said he only put one drop, would it be more convincing that he only had that much myc.

Just curious :confused:


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Offlinescatmanrav
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Re: LC question, PICS [Re: CLUTCH]
    #4298497 - 06/15/05 11:13 AM (12 years, 4 months ago)

>but wouldn't a couple cc's make it colonize faster

By a day or three, sure. If he was saying it had only been a week or two, then it might affect it somewhat...but right here:

"I tried a lc baby bottle about a month ago"

And its been a few weeks after that, and still that shitty growth? No way that the smount of spores has anything to do with that.

Mycelium replicates...think of cell division...a cell divides into 2, 2 divide into 4, 4 into 8, 8 into 16, 16 into 32, 32 into 64, 64 into 128, 128 into 256, 256 into 512....now if each of those divisions happens in a day, how much of a difference would you think it would matter if you start off with 2 cells from the smallest amount of spore solution you could put on, or if it starts off at 32 cells because you put in 16 times the spore solution.

A bit conjumbled, I'm pretty stoned...but I think I need some more bong rips...hope that helps you a little..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineWojo
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Re: LC question, PICS [Re: scatmanrav]
    #4298527 - 06/15/05 11:21 AM (12 years, 4 months ago)

I just need to find a diff way to do LC. I havent had any luck with other ways either though, maybe its just not for me :frown:

~woj


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OfflineCLUTCH
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Re: LC question, PICS [Re: Wojo]
    #4300543 - 06/15/05 07:11 PM (12 years, 4 months ago)

Have you tried the Malt/Dextrose with water combination. Using gas exchange at the top through rubber hose with polyfill?

I am trying it out for the first time. Just inoculated, so no growth yet in this jar. I don't have a plasic lid yet for my jars and I wanted to use plastic, and I was out of mayonaise. So I bought a jar of mayo and emptied it into another jar. The mayo jar worked fine for me to get a jar started. It is just the right size and comes with a plastic lid.



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Invisibleagar
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Re: LC question, PICS [Re: CLUTCH]
    #4300780 - 06/15/05 08:21 PM (12 years, 4 months ago)



1/2 gallon jar (light dry malt & dry dextrose 3% solution)
Rigged with double tube filter lid (polyfil).
Stirred on magnetic stir plate, a few minutes - every other day.
Picture taken prior to stirring.

Note, about 20 % + of liquid is occupied with thick myc mass.
Also note, myc growing on liquids surface.
If not stirred on scedule, surface would turn to thick myc mat.

So, with adiquate gas exchange, evrything grows very well.

At about this stage, it is best to refridgerate the culture, to stall growth.

If you wonder, Lid & filter tubes are covered with double coffee filters - held down by rubber bands, as exterior dust covers. On the top of the coffee filters sets an inverted PP Deli container, as another dust cover - over the coffee filters.

Because, if you allow for maximum gas/air exchange - you want as much of protection - as possible, so nothing unwanted can sneak in & foul the culture. :mad2:



I also use a self loading syringe to asperate culture with & inoculate directly into vacutainers, or spawn containers, to minimize open air contamination vectors.

Once the culture is partialy used, I don't stir of agitate it - for several days. Because, if it contaminated during the asperation process , it - or they will generally show up on the surface, within 4 or 5 days.


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OfflineCLUTCH
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Re: LC question, PICS [Re: Wojo]
    #4301021 - 06/15/05 09:22 PM (12 years, 4 months ago)

There ya go :wink:


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OfflineWojo
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Re: LC question, PICS [Re: CLUTCH]
    #4302145 - 06/16/05 02:10 AM (12 years, 4 months ago)

Sweet, thx for the response, i once tried using malt (i have a shitload of the stuff left, got it cheap) but had no luck, i innoc'd some birdseed with it and got nothing but green mold.. Im thinking of trying the malt again, but using those lil vacutainer lids and having a poly-filled breathing tube. Give that a lil go just to see if i can actually get a descent LC goin.

Thx again
~woj


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Invisibleagar
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Re: LC question, PICS [Re: Wojo]
    #4302159 - 06/16/05 02:14 AM (12 years, 4 months ago)

I use 50/50 light dry malt & dry dextrose to make a 3% solution. PC it, then filter it through coffee filters to remove any solids, crud or silt that forms & PC the filtered stuff again. Best combo I have ever found. Hope you do well, I have.


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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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