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North Spore Shop: Bulk Substrate

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Registered: 02/27/02
Posts: 56
Last seen: 21 years, 7 months
Non-Hole-Lid sterilization
    #566727 - 03/01/02 09:55 AM (21 years, 9 months ago)

With a non-hole-lid innoculation, would having the jar lids loose suffice when steam sterilizing? Previous works with holes in lids provided decent exposure for sterilization, I'm scared now that the holes have not been made, whether a sterile substrate was obtained from steaming?

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Registered: 08/29/01
Posts: 135
Last seen: 21 years, 6 months
Re: Non-Hole-Lid sterilization [Re: SpaceCruiser]
    #566761 - 03/01/02 10:38 AM (21 years, 9 months ago)

Really dont think the holes would have all that much to do with the steralizing process , so you should be o.k , as far as the odds with steam steralizing anyway .. It sounds like your going to open the jars for inculation . Between steam steralizing and opening the lids you have a good chance of contams , you should inculate using the oven ... why not just punch some holes in the lid ?

This statment or question is fictional and for fun not to be used in real life. Just a hypothetical situation..

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North Spore Shop: Bulk Substrate

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