Tightness of Lid during Sterilization (steaming) - Mushroom Cultivation - Shroomery Message Board

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Tightness of Lid during Sterilization (steaming)

#1162804 - 12/22/02 03:39 PM (20 years, 5 months ago)

I forgot to loosen the lids when i steamed my jars for sterilization. Is this going to contribute to the effectiveness of the sterilization.

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Skikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 18 years, 2 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162817 - 12/22/02 03:42 PM (20 years, 5 months ago)

If you have holes in the lids, then no, it shouldn't.

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Re-Defeat Bush in '04

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Anonymous

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162821 - 12/22/02 03:43 PM (20 years, 5 months ago)

I never loosened the lids when I sterilized, and I never put holes in the lids either.

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162822 - 12/22/02 03:44 PM (20 years, 5 months ago)

I have 4 holes sealed with duct tape. Hope this dosent matter..

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162826 - 12/22/02 03:45 PM (20 years, 5 months ago)

Ok, then it shouldn't matter, thanks guys for the help :wink:

:wink:

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funkymonk
Get's down, withthe get-down.

Registered: 11/29/02
Posts: 8,160
Loc: saskatchewan

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162832 - 12/22/02 03:47 PM (20 years, 5 months ago)

That's an area i'm not sure of yet..

I'll be boiling my jars in water to sterilize, now do I have the lids tight, or loose? And the water level? do i want the jar's covered or whats the deal? don't tell me to read the FAQ though I want a first hand answer....

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Skikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 18 years, 2 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: Anonymous]
#1162835 - 12/22/02 03:48 PM (20 years, 5 months ago)

In reply to:

and I never put holes in the lids either.

How do you inoculate, G2G?

--------------------
Re-Defeat Bush in '04

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: funkymonk]
#1162840 - 12/22/02 03:50 PM (20 years, 5 months ago)

Read the PF Tek.

tight or loose, your preference
1/3 - 1/2
aluminium foil

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162854 - 12/22/02 03:54 PM (20 years, 5 months ago)

Skikid16,
Are you familiar with Mass spawning using brf? I was thinking about putting 1 colonized jar into a ziplock oven bag with 2 sterile brf/verm jars, and mixing it up.
You know if oven bags can be steamed? So i can skip a step to reduce contams.

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Skikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 18 years, 2 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162876 - 12/22/02 04:01 PM (20 years, 5 months ago)

In reply to:

Are you familiar with Mass spawning using brf?

Nope. IME, large amounts of BRF takes too long to colonize which ups the chance for contams, I read about the method that you speak of, but if I were to do a bulk substrate for spawn, I would jus use millet.

--------------------
Re-Defeat Bush in '04

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friggin wigga
fungi ingestor

Registered: 11/13/02
Posts: 159
Loc: Trip Land
Last seen: 10 years, 2 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162890 - 12/22/02 04:04 PM (20 years, 5 months ago)

I did a little home experiment to find out which was better, b/c I read both ways to do it. So I did one set(2) with tapes over the holes while steaming, and the other set(2) without the holes covered. I found MUCH faster colonization in the ones w/out the holes covered. I figured it just let's more moisture into the jar, and gives it a stronger chance to survive.

So I would recommend leaving some way to let the steam in, wether keeping the jars loose, or leaving tape off the holes.

--------------------
- Without this precious mana, we wouldn't know ourselves.-

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162899 - 12/22/02 04:07 PM (20 years, 5 months ago)

I was resorting to this method b/c of the lack of a pc ;(
otherwise i would use a wbs or millet.

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Skikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 18 years, 2 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: dankcrop]
#1162916 - 12/22/02 04:11 PM (20 years, 5 months ago)

In reply to:

I was resorting to this method b/c of the lack of a pc

Why not just do a bunch of jars, then spawn them into poo. I think this would be easier than trying to colonize in a ziplock.

--------------------
Re-Defeat Bush in '04

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dankcrop
unknown

Registered: 09/30/02
Posts: 149
Last seen: 14 years, 3 months

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162938 - 12/22/02 04:16 PM (20 years, 5 months ago)

To come to think of it, i've never really read up on dung. Can you hook me up with a link. thanks

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Anonymous

Re: Tightness of Lid during Sterilization (steaming)

[Re: Skikid16]
#1162942 - 12/22/02 04:17 PM (20 years, 5 months ago)

>How do you inoculate, G2G?

I just open the lid up and innoculate.

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Anonymous

Re: Tightness of Lid during Sterilization (steaming)

[Re: Anonymous]
#1180073 - 12/30/02 01:09 PM (20 years, 5 months ago)

I used some wide-mouth "Self-Sealing" mason jars. I read somewhere that if they are "self-sealing", meaning that they have disc-and-ring tops, then you should indeed screw the lids on tight. Make sure if you use these types of jars that you don't poke holes into them until you are ready to innoculate.

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SixCee
keep rolling

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago

Re: Tightness of Lid during Sterilization (steaming)

[Re: daba]
#1180078 - 12/30/02 01:12 PM (20 years, 5 months ago)

For a poo tek look into my topic about small fruits to find the link to Roadkill's topic.

--------------------
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