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Invisiblemungojerry
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Shakable PF Cakes (5 day colonization)
    #5620007 - 05/12/06 02:30 AM (17 years, 8 months ago)

If I were to sterilize some cakes, take them out of there jars (in a glove box) crumble them out of there cake form into basically shakable grains would this speed up the process to 10 days or less like most grains? At least a lot less time than normally needed? Also could I only put half the water content normally added to the pf tek, sterilize, then add enough lc to account for the missing water (preventing an incorrect water ratio)?

With these two things combined could you completely colonize a jar/combination of many jars in less than 5 days?


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OfflineAkamatsu
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5620013 - 05/12/06 02:38 AM (17 years, 8 months ago)

The consistency of PF cakes makes it almost impossible to crumble them in to shakeable pieces, it will just stick together as soon as you let it settle. Just use an LC and good gas exchange if you want to speed up the process using the PF tek. Using 4ccs of LC in each jar, I've had half pint jars colonize in 5 days.


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Invisibledysphoria
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5620016 - 05/12/06 02:40 AM (17 years, 8 months ago)

hrm.
i suppose, that the first issue you want addressed...could theoretically be done. though the whole procedure would have too many vectors to allow new contaminates in. and yes, thats even accounting for the glove box. I'm sure it _could_ be done, dont get me wrong..just that it'd be hard, compared with splurging on a good pc, and investing in a type of grain to begin with.

as for the second issue, you really shouldn't have to inject more than, say, HALF a cc of LC into each inoculation point. i think altering the formula would cause more hindrance than it would solve. just sounds like you're adding too much LC to the pf jars, if you're having moisture problems on the wetter side, for the substrate.

-jb


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Invisiblemungojerry
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Re: Shakable PF Cakes (5 day colonization) [Re: dysphoria]
    #5620035 - 05/12/06 02:57 AM (17 years, 8 months ago)

Quote:

dysphoria said:
though the whole procedure would have too many vectors to allow new contaminates in. and yes, thats even accounting for the glove box



Are you saying it could still contaminate in a glove box? I don't understand how? I figured if I wore gloves sprayed down my glove box with Lysol it'd be damn near 100% contam free....

Quote:

dysphoria said:
just that it'd be hard, compared with splurging on a good pc, and investing in a type of grain to begin with.





well I figured if I want to accurately put less water in and make up for it with lc, the pf tek would be best


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OfflineIGnosticAbhorI
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5620040 - 05/12/06 03:06 AM (17 years, 8 months ago)

Quote:

I figured if I wore gloves sprayed down my glove box with Lysol it'd be damn near 100% contam free....




The only thing that is guaranteed to be CONTAM./Spore free....Is a damn clean room :smirk:

GB's are more sterile...But not 100%.

Quote:

well I figured if I want to accurately put less water in and make up for it with lc, the pf tek would be best




You could do that...But regardless...
Quote:

The consistency of PF cakes makes it almost impossible to crumble them in to shakeable pieces, it will just stick together as soon as you let it settle. Just use an LC and good gas exchange if you want to speed up the process using the PF tek. Using 4ccs of LC in each jar, I've had half pint jars colonize in 5 days.




-Gnostic


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Invisibledysphoria
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5620044 - 05/12/06 03:07 AM (17 years, 8 months ago)

yes, damn near id agree
but, lets say a hypothetical jar had a 100% contam free life.
by taking it out of the jar, in even aseptic conditions, you might give that contamination lurking the .0001 chance it needs to find its way into uncolonized substrate. most ppl prefer to leave it in the jar until its done, less chance of fuckups.

as for altering the formula, minute changes wouldnt make much of a difference. and i suppose it'd work for this case. and yea, you're right, better to alter a pf jar than something else.

-jb


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OfflineIGnosticAbhorI
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Re: Shakable PF Cakes (5 day colonization) [Re: IGnosticAbhorI]
    #5620091 - 05/12/06 03:45 AM (17 years, 8 months ago)

Theortically, anything is impossible.

cept drawing a circular square. :P

-Gnostic


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InvisibleFooMan
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5620167 - 05/12/06 04:53 AM (17 years, 8 months ago)

Why go through all the trouble for a PF cake? The point of using a 1/2 pint jar is that the myc will colonize the substrate more quickly. Using chunkier verm, chunkier rice, incubating at the proper temps and inoculating with an LC will dramatically speed things up without the increased contam risks involved in crumbling a partly colonized cake.


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OfflineIGnosticAbhorI
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Re: Shakable PF Cakes (5 day colonization) [Re: FooMan]
    #5620220 - 05/12/06 05:43 AM (17 years, 8 months ago)

^^ :thumbup:

-Gnostic


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Offlineskeletor
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Re: Shakable PF Cakes (5 day colonization) [Re: IGnosticAbhorI]
    #5620972 - 05/12/06 11:51 AM (17 years, 8 months ago)

also if your are spraying shitloads of lysol in a GB when crumblings cakes in there your gonna end up with some fucked up mutants. im talking ninja turtles here.


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Invisiblemonstermitch
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Re: Shakable PF Cakes (5 day colonization) [Re: skeletor]
    #5620982 - 05/12/06 11:53 AM (17 years, 8 months ago)

ninja turtles?

I'm thinking shit like the x-men with weird powers and the like.

lysol=seriously f@cked-up fruits most times.

seriously


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OfflineYefet2000
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Re: Shakable PF Cakes (5 day colonization) [Re: monstermitch]
    #5622118 - 05/12/06 06:17 PM (17 years, 8 months ago)

Just do grain...its easy fast and above all its so shakeable....:)


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InvisibleHippie3
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Re: Shakable PF Cakes (5 day colonization) [Re: Yefet2000]
    #5622342 - 05/12/06 07:15 PM (17 years, 8 months ago)

just put the brf/pf mixture
in mycrobags
and knead the bags at 30% colonization
to spread the mycellia
just like shaking jars of grains.


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Offlineshroomballa
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Re: Shakable PF Cakes (5 day colonization) [Re: Hippie3]
    #5623284 - 05/12/06 11:22 PM (17 years, 8 months ago)

The last set of jars I made, I shot all but one with 4ccs LC, and one with 7ccs. The 7cc jar colonized the quickest. Keep in mind however, i made the substrate slightly drier. Because LC is so easy to come by (making in rather large batches) I would suggest at least 4ccs highly.


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InvisiblePsychoslut
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Re: Shakable PF Cakes (5 day colonization) [Re: mungojerry]
    #5623830 - 05/13/06 09:21 AM (17 years, 8 months ago)

I posted a thread about this a long time ago. You can do Pf substrate in a quart jar with a tyvek or poly fill filter. Just fill the jar halfway up. Believe it or not if the the substrate is properly made and mixed it will shake fine in the jar. Maybe there will be a couple small clumps but that doesnt matter.


Somebody try it besides me!


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
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Re: Shakable PF Cakes (5 day colonization) [Re: Psychoslut]
    #5623845 - 05/13/06 09:26 AM (17 years, 8 months ago)

sorry, don't want to try it.

pf jar fast colonization for me:

chunkier verm in substrate
fine verm on bottom for soak up and on top for filter
25% of water content is fresh brewed and cooled coffee
substrate is made slightly dryer than recommended
inoculate with 7cc's of Liquid Culture per jar

Boom, no need for any more speed than that.

Otherwise.....

Use Grains.


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InvisiblePsychoslut
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Re: Shakable PF Cakes (5 day colonization) [Re: monstermitch]
    #5623913 - 05/13/06 09:46 AM (17 years, 8 months ago)

yeah ok thanks for the advice!  :rolleyes:


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
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Re: Shakable PF Cakes (5 day colonization) [Re: Psychoslut]
    #5623954 - 05/13/06 09:54 AM (17 years, 8 months ago)

:rockon:


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InvisiblePsychoslut
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Re: Shakable PF Cakes (5 day colonization) [Re: monstermitch]
    #5623986 - 05/13/06 10:02 AM (17 years, 8 months ago)

The Netherlands must not have sarcasm heh? :lol:


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisiblemonstermitch
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Re: Shakable PF Cakes (5 day colonization) [Re: Psychoslut]
    #5624013 - 05/13/06 10:08 AM (17 years, 8 months ago)

no we do.

my guy was a "kill em with kindness" response.

I got what you were saying. And you're for sure right.
There is no right way to do this, per se, and my comment was a bit out of line.

Someone should try it, better yet I should try both side by side and see which one is better so that I can give an informed response, not one like I did.

thanks for setting me straight, I need it semi-frequently.


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