|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
plaastikbob
Stranger

Registered: 09/06/01
Posts: 18
|
Peroxide in Liquid Tissue Culture?
#534870 - 01/29/02 10:29 AM (21 years, 10 months ago) |
|
|
I have read a lot of methods for cloning mushies. Peroxidated agar seems great because of the minimal chances of cantamination, however liquid cloning teks seem much more practical and easy. The only thing is, I haven't been able to find a method for using peroxide in a liquid recipe such as honey or dextrose water. Seems like it would work since in works in agar. Has anyone tried this? If so can you give me details about the recipe you used? Any suggestions would be great. Thanks a lot. PB
-------------------- Any posts by this user is for information only. At no time has this user attempted to grow mushrooms.
|
plaastikbob
Stranger

Registered: 09/06/01
Posts: 18
|
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
#534969 - 01/29/02 12:17 PM (21 years, 10 months ago) |
|
|
So no one has ever tried this?
-------------------- Any posts by this user is for information only. At no time has this user attempted to grow mushrooms.
|
ar393
old timer

Registered: 01/26/00
Posts: 702
Loc: VT
|
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
#535014 - 01/29/02 01:44 PM (21 years, 10 months ago) |
|
|
my cat has never needed to, never had contamination problems using normal or agar or normal hooney water. work clean and you dont need to take extra steps. its a lot easier to work clean thant to keep fucking withthings to try to make things "easier". peace, ar393
|
Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
|
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
#535120 - 01/29/02 04:18 PM (21 years, 10 months ago) |
|
|
Im must trying to find out something similar at the moment - basically im trying to take some sterile tissue from a fruit, and add it to a mix of malt extract, h202 and water ratios 1ml:3ml:100mls -cant see why it wouldnt work
--------------------
|
cookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 21 years, 7 months
|
Re: Peroxide in Liquid Tissue Culture? [Re: ar393]
#535277 - 01/29/02 07:52 PM (21 years, 10 months ago) |
|
|
So you say this liquid culture works well? i just tried some, myself. I mixed 4g malt extract per 96mL of water, and a/c it on the top, i used anno's poly filter floss filters... and innoculated 2 jars with spores, and 2 with pieces of mycelium from agar.. what do you think fo that how long would 150mL of water take to colonize from spores? from mycelium? is my air exchange sufficient?
|
Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
|
Re: Peroxide in Liquid Tissue Culture? [Re: cookiewhore]
#535620 - 01/30/02 03:29 AM (21 years, 10 months ago) |
|
|
check my post in advanced mushroom cultivation about cloning without agar
--------------------
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,682 topic views. 50 members, 181 guests and 72 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|