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Invisibleplaastikbob
Stranger

Registered: 09/06/01
Posts: 18
Peroxide in Liquid Tissue Culture?
    #534870 - 01/29/02 12:29 PM (15 years, 5 months ago)

I have read a lot of methods for cloning mushies. Peroxidated agar seems great because of the minimal chances of cantamination, however liquid cloning teks seem much more practical and easy. The only thing is, I haven't been able to find a method for using peroxide in a liquid recipe such as honey or dextrose water. Seems like it would work since in works in agar. Has anyone tried this? If so can you give me details about the recipe you used? Any suggestions would be great. Thanks a lot. PB


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Any posts by this user is for information only. At no time has this user attempted to grow mushrooms.


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Invisibleplaastikbob
Stranger

Registered: 09/06/01
Posts: 18
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
    #534969 - 01/29/02 02:17 PM (15 years, 5 months ago)

So no one has ever tried this?


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Invisiblear393
old timer
Male
Registered: 01/27/00
Posts: 702
Loc: VT
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
    #535014 - 01/29/02 03:44 PM (15 years, 5 months ago)

my cat has never needed to, never had contamination problems using normal or agar or normal hooney water.
work clean and you dont need to take extra steps. its a lot easier to work clean thant to keep fucking withthings to try to make things "easier".
peace, ar393


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Peroxide in Liquid Tissue Culture? [Re: plaastikbob]
    #535120 - 01/29/02 06:18 PM (15 years, 5 months ago)

Im must trying to find out something similar at the moment - basically im trying to take some sterile tissue from a fruit, and add it to a mix of malt extract, h202 and water ratios 1ml:3ml:100mls -cant see why it wouldnt work


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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 15 years, 3 months
Re: Peroxide in Liquid Tissue Culture? [Re: ar393]
    #535277 - 01/29/02 09:52 PM (15 years, 5 months ago)

So you say this liquid culture works well?
i just tried some, myself. I mixed 4g malt extract per 96mL of water, and a/c it
on the top, i used anno's poly filter floss filters...
and innoculated 2 jars with spores, and 2 with pieces of mycelium from agar..
what do you think fo that
how long would 150mL of water take to colonize from spores? from mycelium?
is my air exchange sufficient?


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Peroxide in Liquid Tissue Culture? [Re: cookiewhore]
    #535620 - 01/30/02 05:29 AM (15 years, 5 months ago)

check my post in advanced mushroom cultivation about cloning without agar


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