Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

PlanetSpores.ca
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   MagicBag.co Certified Organic All-In-One Grow Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Live Tissue Culture
    #406899 -

I recently decided to to start a live tissue culture from a mature mushroom. I split open a mushroom and cut out the tissue from the inside an dplaced it on a culture plate. Unfortunately, it's haas been two weeks not and the growth is little. I'm not sure if the strain is old or if I'm just being impatient. The mushroom was a third flush mushroom but the jars were spore incoulated. Any ideas or suggestions?



--------------------
Quis custodiet ipsos custodes?

Extras: Filter Print Post Top
Re: Live Tissue Culture [Re: Hiroller]
    #406942 -

HaHaHa

I thought you were going to post something about injecting spores into your bloodstream or something. funny.

Ok, to your post:
1) What medium are you using as culture
2) How old is the mushroom? and Is it fresh or dry?
3) How firmly did you push the piece of cap into the medium?
4) How clean were you? (That sounds dumb, but think about it...) It requires as much if not more sterilization as innoculating jars, because the medium is more nutritious than a substrate in a jar. This begs for contamination.
5) Average temperature of dishes?

That's it... Although I havn't utilized this method before, I'm starting some PDY broth jars either sat or sun using some mycelium from my colonized jars that are going to be cased this weekend too. I have read the tek i'm pretty sure you are using so many times I could recite it from memory... So I know it very well.

cheers,

abudiwa

-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
"What? No! We can't stop here!
This is bat country!"
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Shroomr4life@hotmail.com


--------------------
*user out of date*

the only pms pants gets:
<pants> "WTF UR PIKTUR IS SO STIPID WTF FUCK U"

Extras: Filter Print Post Top
Re: Live Tissue Culture [Re: Hiroller]
    #406996 -

Two weeks on a culture plate without growth means you need to try again. Are you using agar? You didn't mention what you were using to grow the live tissue on. Also, did you only prepare one plate? If so, you should always do three or four since not all of them will grow. If you fail with agar, you can grow out live tissue on regular substrate as well. Just prepare the substrate, pack it into a jar (or even a petri dish) and using a scalpel, press tissue from the cap into the substrate. Cover it and watch it grow...


Extras: Filter Print Post Top
Re: Live Tissue Culture [Re: Abudiwa]
    #407807 -

I used Bob's Malt Agar from the Mushroompeople. It's made from malt(never could have guessed huh? =) ), cornsteep, Calcium Hydroxide, and Potassium Phosphate. The mushroom had just been picked when I placed it on the original agar but then I transfered it to isolate the strain from contaminates that began to appear on the plate. As for pushing it into the agar, I never knew I had to something as such. I usually just place the agar wedge or tissue on top of te agar. I typically leave it incubated right around 84 to 86 degrees, never going above 86 and not below a low of 82.
TO give you a better idea of what has happened, I transfered the 3 wedges from the orginal plate to 3 different plates. I kinda left them alone for two weeks and when I checked them the mycelium had barely grown off the transfered wedges and onto the new agar.
Does pushing the wedges/tissue really matter that much? I mean, it could be that or maybe the agar isn't good for cubes. When I tried to do strain selection with spores, the spores grew into mycelium, but the mycelium was rather weak looking and again didn't grow that far.
PLUR



--------------------
Quis custodiet ipsos custodes?

Extras: Filter Print Post Top
Re: Live Tissue Culture [Re: Abudiwa]
    #407808 -

I used Bob's Malt Agar from the Mushroompeople. It's made from malt(never could have guessed huh? =) ), cornsteep, Calcium Hydroxide, and Potassium Phosphate. The mushroom had just been picked when I placed it on the original agar but then I transfered it to isolate the strain from contaminates that began to appear on the plate. As for pushing it into the agar, I never knew I had to something as such. I usually just place the agar wedge or tissue on top of te agar. I typically leave it incubated right around 84 to 86 degrees, never going above 86 and not below a low of 82.
TO give you a better idea of what has happened, I transfered the 3 wedges from the orginal plate to 3 different plates. I kinda left them alone for two weeks and when I checked them the mycelium had barely grown off the transfered wedges and onto the new agar.
Does pushing the wedges/tissue really matter that much? I mean, it could be that or maybe the agar isn't good for cubes. When I tried to do strain selection with spores, the spores grew into mycelium, but the mycelium was rather weak looking and again didn't grow that far.
PLUR

P.S. I was going to try to inject myself with some spores but I caught a glimpse of a picture on this website of a shaman with mushrooms with fingers. I thought that would be cool until I realized I would have family and friends gnawing on my hands, Heh



--------------------
Quis custodiet ipsos custodes?

Extras: Filter Print Post Top
Re: Live Tissue Culture [Re: Hiroller]
    #408006 -

Did you let the agar cool completely before placing the tissue on it? You may need to experiment with different incubation temps. I've successfully grown out tissue on agar at room temperature as well as 84F. Try it again and see what happens. If at first you don't succeed...


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   MagicBag.co Certified Organic All-In-One Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Potassium Hydroxide as a pH buffer in a casing discman1 1,899 14 11/11/04 05:22 AM
by Sam1912
* Yello stuff on plant Tissue culture (Agar) Anand 1,604 10 03/28/03 12:22 PM
by Anno
* Funkyballoon's NEW Tissue Culture Technique??? KitMarlowe 1,978 2 01/08/02 11:43 AM
by Visigoth
* tissue culture...? 123dhs 804 3 05/30/04 10:54 PM
by NewfoundFreedom
* Factors in achieving rhizomorhic growth on agar NDK 2,075 2 10/12/01 11:50 AM
by marx2k
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )		 agar 56,348 43 07/08/23 07:04 AM
by Screwup
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )		 funkyballoon 22,588 38 05/11/01 11:08 AM
by MAIA
* collecting spores/tissue culture from the veil? GestaltAssault 919 6 08/13/03 07:09 PM
by MycoCakeEater

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,072 topic views. 21 members, 295 guests and 85 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 14 queries.