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OfflineMklangelo
enthusiast
Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 1 month
Liquid Mycellial Culture Question :-)
    #529984 - 01/24/02 09:16 AM (22 years, 1 month ago)

Will a jar innoculated with a tissue culture liquid colonize faster than one done with simply a Mycellial Honey Water? Please assume of course that all other things are equal.



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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 2 months
Re: Liquid Mycellial Culture Question :-) [Re: Mklangelo]
    #530037 - 01/24/02 10:01 AM (22 years, 1 month ago)

A mycellium liquid culture will start to colonize a substrate immediately after inoculation. A liquid tissue culture is in a sterile solution to prevent mycellial growth, it will require an extra day for colonizing to begin. Depending on the strain, some liquid tissue cultures could start to form mycellium within hours.

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InvisibleSouthernGent
veteran
Registered: 01/12/02
Posts: 1,331
Re: Liquid Mycellial Culture Question :-) [Re: Shdwstr]
    #530041 - 01/24/02 10:07 AM (22 years, 1 month ago)

really? can you buy liquid or is it legal?


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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 2 months
Re: Liquid Mycellial Culture Question :-) [Re: SouthernGent]
    #530063 - 01/24/02 10:27 AM (22 years, 1 month ago)

Sorry... you'll have to make your own liquid cultures.

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OfflineMklangelo
enthusiast
Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 1 month
Re: Liquid Mycellial Culture Question :-) [Re: Shdwstr]
    #530090 - 01/24/02 11:02 AM (22 years, 1 month ago)

I knew this dude that said it used to work like a charm with his Cambodians. Was he exagerating? Of course he was reffering to the Tissue Culture method.


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Edited by Mklangelo (01/24/02 11:04 AM)

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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 2 months
Re: Liquid Mycellial Culture Question :-) [Re: Mklangelo]
    #530101 - 01/24/02 11:16 AM (22 years, 1 month ago)

He wasn't exagerating. Mycellium liquid cultures colonize quickly, overcoming contams. By using a tissue culture you get the added benifits of more uniform flushes and fruits due to the cloning.
You can also make a mycellium culture using your tissue culture.

Excellent results!

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Invisiblear393
old timer
Male
Registered: 01/26/00
Posts: 702
Loc: VT
Re: Liquid Mycellial Culture Question :-) [Re: Shdwstr]
    #530133 - 01/24/02 12:08 PM (22 years, 1 month ago)

umm, once you have they honey water going, what do you think it is? its a liquid mycelium culture....#$#%$&^$
peace, ar393

Edited by ar393 (01/24/02 12:47 PM)

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OfflineMklangelo
enthusiast
Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 1 month
Re: Liquid Mycellial Culture Question :-) [Re: ar393]
    #530247 - 01/24/02 02:34 PM (22 years, 1 month ago)

Yes, but not a Liquid TISSUE culture.

We were just differentiating between liquid cultures produced from spores and those from tissue.

:-)


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Invisiblear393
old timer
Male
Registered: 01/26/00
Posts: 702
Loc: VT
Re: Liquid Mycellial Culture Question :-) [Re: Mklangelo]
    #530829 - 01/25/02 06:40 AM (22 years, 1 month ago)

:smile:

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