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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Why do agar?
    #5032931 -

I understand that agar is a good growth medium for mycelium and is great for cloning, separating a strain from contams, and allows you to choose the most prolific mycelium.

What I don't understand if your not cloning, and are pretty confident your spores are contam free why do agar work? Does choosing the most prolific mycelium and transferring it to a grain have a lot more benefits than just inoculating grain with LC?

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Re: Why do agar? [Re: indianprincess]
    #5032972 -

yes. You can use a couple spores to start one plate, then transfer several cubes of the mycelium to different jars. When your plate is almost out of mycelium, you can transfer another block to another plate to start the cycle again.


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-]Stoner4life[-
4 * 2 * 0
That funky, skunky, smelling green shit
Sing my song, puff all night long....
As I take hits of the bong.

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Re: Why do agar? [Re: indianprincess]
    #5032977 -

indianprincess said:
Does choosing the most prolific mycelium and transferring it to a grain have a lot more benefits than just inoculating grain with LC?


definitely.

when u are doing ms on agar, u can sector and isolate the best growing
substrains from the dish and create a somewhat pure culture of it.

thereby eliminating slow growing, non rhizomorphic strains and just going
for the fast and strong genetics u are aiming for.

if u inoculate using a ms syringe, u cannot chose as there will be millions of
different substrains - lacking the specific characteristics of the one isolated sub.
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Re: Why do agar? [Re: indianprincess]
    #5033008 -

because he is a sexy mofo?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Why do agar? [Re: ohmatic]
    #5033236 -

so once you isolate the substrain and all that good stuff.. and you get prints from your first flush, will the prints keep all the characteristics that you got from doing the agar to begin with or dose it start all over every time you do a MS inoculation??


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:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

"are you sure u want to eat all them shrooms??"  "yes i want to hear what my mind has to say..."

remember opions are like assholes, everyone has one and they all stink.

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Re: Why do agar? [Re: willmafingerdo]
    #5033244 -

it will start over.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Why do agar? [Re: Psychoslut]
    #5033252 -

why?? if the strain was isolated why dont it keep the same characteristics?? i know there are millions of spores in each cap but i thought that with agar you would be pulling out "1" strain or characteristics out of the million.. i think im just babbling.. pay no mind to me.. :smile:


--------------------
:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

"are you sure u want to eat all them shrooms??"  "yes i want to hear what my mind has to say..."

remember opions are like assholes, everyone has one and they all stink.

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Re: Why do agar? [Re: Psychoslut]
    #5033268 -

Yes the only way you can stick with those genetics is to store that culture.
Apparently you can keep it active for a very long time if you periodically switch the nutrient base that you use for you agar recipe. Keeping cultures on the same nutrient base for a long period of time will lead to the mycelium developing more specific enzymes.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

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Re: Why do agar? [Re: musher_420]
    #5033272 -

Look at spores as + and - . Hyphae fusion occurs between + and -. So when + finds - a whole new set of genetics is developed.

A good link
http://www.shroomery.org/forums/files/102702-5/65713-Mushcycle.jpg


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

Edited by musher_420 (12/08/05 11:19 AM)

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Re: Why do agar? [Re: musher_420]
    #5033282 -

with a multispore inoculation you will get traits from the parent mushroom. the mutations will cancelout or be overrun with the more common spores. its like taking the average of all the spores.


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xxx..Learn Something..xxx

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Re: Why do agar? [Re: Omnicracker]
    #5033719 -

this answered some questions i myself had on the agar procedures.


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No matter what I say and no matter what I write here.

I'm sick of always looking at this page with a blank stare.

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Re: Why do agar? [Re: ShroomArtist84]
    #5034910 -

Thanks for all the cool answers!

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Re: Why do agar? [Re: indianprincess]
    #5034924 -

also if you do agar a head of time you can have a lot fast colonization. Plus you can store them in a fridge for use later.

WB


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Re: Why do agar? [Re: WhiteBunny]
    #5034961 -

I enjoy being done. But, not refrigerated. (joke) :grin:


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Re: Why do agar? [Re: agar]
    #5035534 -

can agar cultures be done in on the bottom of a 1/2 pint jar? I don't have petri dishes.


--------------------
Look within.
Be still.
Free from fear and attachment
know the sweet joy of the way.
Buddha


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Re: Why do agar? [Re: Boondock]
    #5035553 -

for sure.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Why do agar? [Re: Psychoslut]
    #5035580 -

one more question,

The Asian market I shop at has, believe it or not, a dried seaweed aisle. They have bags of agar. It looks like clear flakes. Is this the same thing used in agar recipes in the "FAQ's" section?


--------------------
Look within.
Be still.
Free from fear and attachment
know the sweet joy of the way.
Buddha


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Re: Why do agar? [Re: Boondock]
    #5035585 -

Yes.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: Why do agar? [Re: indianprincess]
    #5035688 -

nobody mentioned that...

Agar is lots and lots of fun!

you can really get a good visual sense of what you're dealing with.
I used agar to isolate a pure clean strain from a wild print of p. caerulipes... i've yet to do anything with it yet... but by god it was a blast!

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