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gsmith
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Registered: 12/07/04
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Agar question
#3826607 - 02/24/05 09:00 AM (18 years, 11 months ago) |
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If one were to use Bob's Red Mill Potato Starch powder instead of boiling down potatoes when making agar, how much would one use? And is this stuff a good substitute for real potatoes/flakes? Anybody used it before? It's good in gravy BTW.
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: Agar question [Re: gsmith]
#3826653 - 02/24/05 09:22 AM (18 years, 11 months ago) |
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I do not think flakes would work well , you only want the water that contains all the starches for agar mediums . The starch stuff you mentioned may work, have no idea though. Stick to know recipes for first time around , then exp with your own brew . GL
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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gsmith
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5g of flakes are listed in the PDA recipe as an acceptible substitute to using 150g of sliced potatoes, but i've never used them before. this isn't my first time making agar though. probably should have mentioned that. I'm just asking because I'd rather save time not having to boil the potatoes, and this stuff is cheap too.
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Prisoner#1
Even Dumber ThanAdvertized!


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Quote:
tripndicular said: I do not think flakes would work well
then we should remove that potato flake agar recipe from the FAQ... 
the starch should work but you may have to experiment for a good mixture
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Sorry did not know was a substituted item , but I am lazy type and buy pre made agars(powdered form) myself so go figure  And when making dinner I hate fake textured taters 
Hey was not a wasted day... I learned something new
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
Edited by tripndicular (02/24/05 09:34 AM)
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gsmith
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I'd love some powdered MEA or something... but i need agar now-ish... i just found something that I really really want to culture... I know I've posted earlier saying that I was quitting the game but I have to preserve this specimen. I wish I had a camera to take a picture.
I was cleaning up some crap that I wanted to throw out and I found a jar that I had inoculated on this last run. I used a syringe that I knew for a fact was contaminated with black mold. I had gotten two syringes from the same batch (B+, 3rd party) and all the jars that I innocked with the first syringe contammed. So when I used this second one I just said screw it and put all 12ccs into a single quart jar. Of course it contammed, and rather quickly too. So I poured a bit of .3% h2o2 into it and put it on the back porch and forgot about it. That was about five weeks ago. Yesterday I found shrooms growing inside of it. There's six total. The biggest has a seven inch stem and a three inch cap. Impressive for in vitro... very impressive for growing out of the contams with absolutely no help from me.
I'm not going to eat it, but I want to isolate a tissue culture from the inside of the stem. Hopefully it will be clean enough for me to do this. The black mold in this jar was so thick that it literally looked like a bunch of ground up charcoal. I left it for dead. This suckers a survivor.
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scatmanrav
Brainy Smurf

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Re: Agar question [Re: gsmith]
#3832450 - 02/25/05 10:37 AM (18 years, 10 months ago) |
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That shit happens if you leave all your contamed jars after some time...something will sprout..

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Damn_Skippy
Fell off theWagon

Registered: 01/25/05
Posts: 403
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I was trying to eat saw those pics and almost puked
-------------------- ~Skipp "I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."
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scatmanrav
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Awsome
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gsmith
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Last seen: 18 years, 1 month
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UPDATE: Re: Agar question [Re: scatmanrav]
#3886435 - 03/08/05 08:26 AM (18 years, 10 months ago) |
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UPDATE:
Potato starch is a bitch to work with. I still don't think I have the ammounts right and I made a couple batches. It comes out a lot thicker than the PDA I've made from scratch. It also had a lot more silt settle on the bottom. On the plus though, the tissue I dropped on it cultured nicely.
Tip: Use a food processor to fully incorporate all the ingredients. Potato starch clumps a lot and is hard to whisk apart.
I used: 500ml of boiling water 10g of Bob's Red Mill Potato Starch powder (probably too much) 10g of dextrose 5g of soy flour (had some, thought i'd use it) 5g of nutritional yeast 9g of agar
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Anno
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UPDATE: Re: Agar question [Re: gsmith]
#3886456 - 03/08/05 08:37 AM (18 years, 10 months ago) |
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The point of using potato water is to provide micro nutrients, minerals and proteins to the agar formulation.
The potato starch lacks all of those, it is a refined product, it is no substitute at all for potato water.
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gsmith
reformed lurker
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UPDATE: Re: Agar question [Re: Anno]
#3891633 - 03/09/05 07:07 AM (18 years, 10 months ago) |
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well it worked... refined or not.
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Roadkill
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Registered: 12/11/01
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Re: Agar question [Re: gsmith] 1
#4615552 - 09/03/05 05:52 AM (18 years, 4 months ago) |
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I don't like boiling down potatoes either...
This is the recipy that I use...
-PDYA- 500ml water 5 grams of instant potato flakes(Potato Buds Brand) 9 grams agar agar 10 ml honey (Raw natural pure honey - Beemaid Brand) 1 gram of Nutritional Yeast
works great!~
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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