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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Agar and Liquid Culture
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Agar question
    #3826607 -

If one were to use Bob's Red Mill Potato Starch powder instead of boiling down potatoes when making agar, how much would one use? And is this stuff a good substitute for real potatoes/flakes? Anybody used it before? It's good in gravy BTW.


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Re: Agar question [Re: gsmith]
    #3826653 -

I do not think flakes would work well , you only want the water that contains all the starches for agar mediums . The starch stuff you mentioned may work, have no idea though. Stick to know recipes for first time around , then exp with your own brew .
GL


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Re: Agar question [Re: tripndicular]
    #3826669 -

5g of flakes are listed in the PDA recipe as an acceptible substitute to using 150g of sliced potatoes, but i've never used them before. this isn't my first time making agar though. probably should have mentioned that. I'm just asking because I'd rather save time not having to boil the potatoes, and this stuff is cheap too.


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Re: Agar question [Re: tripndicular]
    #3826672 -

tripndicular said:
I do not think flakes would work well


then we should remove that potato flake agar recipe from the FAQ... :smirk:

the starch should work but you may have to experiment for a good mixture


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California Has Legalized Child Prostitution

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Re: Agar question [Re: Prisoner#1]
    #3826678 -

Sorry did not know was a substituted item , but I am lazy type and buy pre made agars(powdered form) myself so go figure :wink:
And when making dinner I hate fake textured taters :smirk:

Hey was not a wasted day... I learned something new  :thumbup: :cool: :wink:


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

Edited by tripndicular (02/24/05 09:34 AM)

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Re: Agar question [Re: tripndicular]
    #3829402 -

I'd love some powdered MEA or something... but i need agar now-ish... i just found something that I really really want to culture... I know I've posted earlier saying that I was quitting the game but I have to preserve this specimen. I wish I had a camera to take a picture.

I was cleaning up some crap that I wanted to throw out and I found a jar that I had inoculated on this last run. I used a syringe that I knew for a fact was contaminated with black mold. I had gotten two syringes from the same batch (B+, 3rd party) and all the jars that I innocked with the first syringe contammed. So when I used this second one I just said screw it and put all 12ccs into a single quart jar. Of course it contammed, and rather quickly too. So I poured a bit of .3% h2o2 into it and put it on the back porch and forgot about it. That was about five weeks ago. Yesterday I found shrooms growing inside of it. There's six total. The biggest has a seven inch stem and a three inch cap. Impressive for in vitro... very impressive for growing out of the contams with absolutely no help from me.

I'm not going to eat it, but I want to isolate a tissue culture from the inside of the stem. Hopefully it will be clean enough for me to do this. The black mold in this jar was so thick that it literally looked like a bunch of ground up charcoal. I left it for dead. This suckers a survivor.


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Re: Agar question [Re: gsmith]
    #3832450 -

That shit happens if you leave all your contamed jars after some time...something will sprout..



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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
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Re: Agar question [Re: scatmanrav]
    #3832456 -

I was trying to eat saw those pics and almost puked


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~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."

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Re: Agar question [Re: Damn_Skippy]
    #3832669 -

Awsome :smile:


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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UPDATE: Re: Agar question [Re: scatmanrav]
    #3886435 -

UPDATE:

Potato starch is a bitch to work with. I still don't think I have the ammounts right and I made a couple batches. It comes out a lot thicker than the PDA I've made from scratch. It also had a lot more silt settle on the bottom. On the plus though, the tissue I dropped on it cultured nicely.

Tip: Use a food processor to fully incorporate all the ingredients. Potato starch clumps a lot and is hard to whisk apart.

I used:
500ml of boiling water
10g of Bob's Red Mill Potato Starch powder (probably too much)
10g of dextrose
5g of soy flour (had some, thought i'd use it)
5g of nutritional yeast
9g of agar


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UPDATE: Re: Agar question [Re: gsmith]
    #3886456 -

The point of using potato water is to provide micro nutrients, minerals and proteins to the agar formulation.

The potato starch lacks all of those, it is a refined product, it is no substitute at all for potato water.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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UPDATE: Re: Agar question [Re: Anno]
    #3891633 -

well it worked... refined or not.


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Re: Agar question [Re: gsmith] * 1
    #4615552 -

I don't like boiling down potatoes either...

This is the recipy that I use...

-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast


works great!~

tc


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!

Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Agar and Liquid Culture
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